The dynamics of neutrophils in zebrafish (Danio rerio) during infection with the parasite Ichthyophthirius multifiliis
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The dynamics of neutrophils in zebrafish (Danio rerio) during infection with the parasite Ichthyophthirius multifiliis. / Jørgensen, Louise von Gersdorff.
I: Fish and Shellfish Immunology, Bind 55, 2016, s. 159-164.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The dynamics of neutrophils in zebrafish (Danio rerio) during infection with the parasite Ichthyophthirius multifiliis
AU - Jørgensen, Louise von Gersdorff
PY - 2016
Y1 - 2016
N2 - Ichthyophthirius multifiliis is a ciliated protozoan parasite infecting the skin and gills of freshwater fish. Neutrophils are attracted to the infection sites, as a part of the innate immune response. In this study a transgenic line of zebrafish (Tg(MPO:GFP)i114) with GFP-tagged neutrophils was infected with I. multifiliis and the neutrophil influx in the caudal fin was quantified. Twenty-four hours post infection (pi) the neutrophil count had gone up with an average of 3.4 fold. Forty-eight h pi the neutrophil count had dropped 12% and 72 h pi it had dropped to 21% compared to 24 h pi. At 72 h pi the neutrophil count was 2.7 times higher than prior to infection. A few dead parasites were observed, which were disintegrated and covered internally and externally with neutrophils. Live parasites, both surrounded by neutrophils and with no neutrophils in the near vicinity, were found during the infection. Neutrophils interacted directly with the parasites with pseudopod formation projecting towards the pathogen. These results indicate a strong innate immune response immediately following infection and/or a subsequent immune evasion by the parasite.
AB - Ichthyophthirius multifiliis is a ciliated protozoan parasite infecting the skin and gills of freshwater fish. Neutrophils are attracted to the infection sites, as a part of the innate immune response. In this study a transgenic line of zebrafish (Tg(MPO:GFP)i114) with GFP-tagged neutrophils was infected with I. multifiliis and the neutrophil influx in the caudal fin was quantified. Twenty-four hours post infection (pi) the neutrophil count had gone up with an average of 3.4 fold. Forty-eight h pi the neutrophil count had dropped 12% and 72 h pi it had dropped to 21% compared to 24 h pi. At 72 h pi the neutrophil count was 2.7 times higher than prior to infection. A few dead parasites were observed, which were disintegrated and covered internally and externally with neutrophils. Live parasites, both surrounded by neutrophils and with no neutrophils in the near vicinity, were found during the infection. Neutrophils interacted directly with the parasites with pseudopod formation projecting towards the pathogen. These results indicate a strong innate immune response immediately following infection and/or a subsequent immune evasion by the parasite.
KW - Faculty of Health and Medical Sciences
KW - Ichthyophthirius multifiliis
KW - Zebrafish
KW - GFP-tagged neutrophils
KW - Neutrophil influx
KW - Immune evasion
U2 - 10.1016/j.fsi.2016.05.026
DO - 10.1016/j.fsi.2016.05.026
M3 - Journal article
C2 - 27231191
VL - 55
SP - 159
EP - 164
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
SN - 1050-4648
ER -
ID: 161991356