The contraction induced increase in gene expression of peroxisome proliferator-activated receptor (PPAR)-gamma coactivator 1alpha (PGC-1alpha), mitochondrial uncoupling protein 3 (UCP3) and hexokinase II (HKII) in primary rat skeletal muscle cells is dependent on reactive oxygen species

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Standard

The contraction induced increase in gene expression of peroxisome proliferator-activated receptor (PPAR)-gamma coactivator 1alpha (PGC-1alpha), mitochondrial uncoupling protein 3 (UCP3) and hexokinase II (HKII) in primary rat skeletal muscle cells is dependent on reactive oxygen species. / Silveira, Leonardo R.; Pilegaard, Henriette; Kusuhara, Keiko; Curi, Rui; Hellsten, Ylva.

I: BBA General Subjects, Bind 1763, Nr. 9, 2006, s. 969-976.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Silveira, LR, Pilegaard, H, Kusuhara, K, Curi, R & Hellsten, Y 2006, 'The contraction induced increase in gene expression of peroxisome proliferator-activated receptor (PPAR)-gamma coactivator 1alpha (PGC-1alpha), mitochondrial uncoupling protein 3 (UCP3) and hexokinase II (HKII) in primary rat skeletal muscle cells is dependent on reactive oxygen species', BBA General Subjects, bind 1763, nr. 9, s. 969-976. https://doi.org/10.1016/j.bbamcr.2006.06.010

APA

Silveira, L. R., Pilegaard, H., Kusuhara, K., Curi, R., & Hellsten, Y. (2006). The contraction induced increase in gene expression of peroxisome proliferator-activated receptor (PPAR)-gamma coactivator 1alpha (PGC-1alpha), mitochondrial uncoupling protein 3 (UCP3) and hexokinase II (HKII) in primary rat skeletal muscle cells is dependent on reactive oxygen species. BBA General Subjects, 1763(9), 969-976. https://doi.org/10.1016/j.bbamcr.2006.06.010

Vancouver

Silveira LR, Pilegaard H, Kusuhara K, Curi R, Hellsten Y. The contraction induced increase in gene expression of peroxisome proliferator-activated receptor (PPAR)-gamma coactivator 1alpha (PGC-1alpha), mitochondrial uncoupling protein 3 (UCP3) and hexokinase II (HKII) in primary rat skeletal muscle cells is dependent on reactive oxygen species. BBA General Subjects. 2006;1763(9):969-976. https://doi.org/10.1016/j.bbamcr.2006.06.010

Author

Silveira, Leonardo R. ; Pilegaard, Henriette ; Kusuhara, Keiko ; Curi, Rui ; Hellsten, Ylva. / The contraction induced increase in gene expression of peroxisome proliferator-activated receptor (PPAR)-gamma coactivator 1alpha (PGC-1alpha), mitochondrial uncoupling protein 3 (UCP3) and hexokinase II (HKII) in primary rat skeletal muscle cells is dependent on reactive oxygen species. I: BBA General Subjects. 2006 ; Bind 1763, Nr. 9. s. 969-976.

Bibtex

@article{ff13ad90966311dbbee902004c4f4f50,
title = "The contraction induced increase in gene expression of peroxisome proliferator-activated receptor (PPAR)-gamma coactivator 1alpha (PGC-1alpha), mitochondrial uncoupling protein 3 (UCP3) and hexokinase II (HKII) in primary rat skeletal muscle cells is dependent on reactive oxygen species",
abstract = "We evaluated the role of reactive oxygen species (ROS) for the contraction induced increase in expression of PGC-1alpha, HKII and UCP3 mRNA. Rat skeletal muscle cells were subjected to acute or repeated electrostimulation in the presence and absence of antioxidants. Contraction of muscle cells lead to an increased H2O2 formation, as measured by oxidation of H2HFF. Acute contraction of the muscle cells lead to a transient increase in PGC-1alpha and UCP3 mRNA by 172 and 65%, respectively (p<0.05), whereas this increase was absent in the presence of antioxidants. Repeated contraction sessions induced a sustained elevation in PGC-1alpha and UCP3 mRNA and a transient increase in HKII (p<0.05) and this effect was not present with treatment of cells with either an antioxidant cocktail or with GPX+GSH. Incubation of cells for 10 days with ROS produced by xanthine oxidase/xanthine increased the level of PGC-1alpha, HKII and UCP3 mRNA by 175, 58 and 115%, respectively (p<0.05). A 10-day incubation of cells with antioxidants was found to have no effect on the basal mRNA content (p>0.05). The present data demonstrate that contraction of skeletal muscle cells leads to an enhanced formation of ROS and an elevation in PGC-1alpha, UCP3 and HKII mRNA content which is abolished in the presence of antioxidants, suggesting that ROS are of importance for the contraction induced increase in expression of these genes in skeletal muscle.",
author = "Silveira, {Leonardo R.} and Henriette Pilegaard and Keiko Kusuhara and Rui Curi and Ylva Hellsten",
note = "PUF 2006 5200 020",
year = "2006",
doi = "10.1016/j.bbamcr.2006.06.010",
language = "English",
volume = "1763",
pages = "969--976",
journal = "B B A - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "9",

}

RIS

TY - JOUR

T1 - The contraction induced increase in gene expression of peroxisome proliferator-activated receptor (PPAR)-gamma coactivator 1alpha (PGC-1alpha), mitochondrial uncoupling protein 3 (UCP3) and hexokinase II (HKII) in primary rat skeletal muscle cells is dependent on reactive oxygen species

AU - Silveira, Leonardo R.

AU - Pilegaard, Henriette

AU - Kusuhara, Keiko

AU - Curi, Rui

AU - Hellsten, Ylva

N1 - PUF 2006 5200 020

PY - 2006

Y1 - 2006

N2 - We evaluated the role of reactive oxygen species (ROS) for the contraction induced increase in expression of PGC-1alpha, HKII and UCP3 mRNA. Rat skeletal muscle cells were subjected to acute or repeated electrostimulation in the presence and absence of antioxidants. Contraction of muscle cells lead to an increased H2O2 formation, as measured by oxidation of H2HFF. Acute contraction of the muscle cells lead to a transient increase in PGC-1alpha and UCP3 mRNA by 172 and 65%, respectively (p<0.05), whereas this increase was absent in the presence of antioxidants. Repeated contraction sessions induced a sustained elevation in PGC-1alpha and UCP3 mRNA and a transient increase in HKII (p<0.05) and this effect was not present with treatment of cells with either an antioxidant cocktail or with GPX+GSH. Incubation of cells for 10 days with ROS produced by xanthine oxidase/xanthine increased the level of PGC-1alpha, HKII and UCP3 mRNA by 175, 58 and 115%, respectively (p<0.05). A 10-day incubation of cells with antioxidants was found to have no effect on the basal mRNA content (p>0.05). The present data demonstrate that contraction of skeletal muscle cells leads to an enhanced formation of ROS and an elevation in PGC-1alpha, UCP3 and HKII mRNA content which is abolished in the presence of antioxidants, suggesting that ROS are of importance for the contraction induced increase in expression of these genes in skeletal muscle.

AB - We evaluated the role of reactive oxygen species (ROS) for the contraction induced increase in expression of PGC-1alpha, HKII and UCP3 mRNA. Rat skeletal muscle cells were subjected to acute or repeated electrostimulation in the presence and absence of antioxidants. Contraction of muscle cells lead to an increased H2O2 formation, as measured by oxidation of H2HFF. Acute contraction of the muscle cells lead to a transient increase in PGC-1alpha and UCP3 mRNA by 172 and 65%, respectively (p<0.05), whereas this increase was absent in the presence of antioxidants. Repeated contraction sessions induced a sustained elevation in PGC-1alpha and UCP3 mRNA and a transient increase in HKII (p<0.05) and this effect was not present with treatment of cells with either an antioxidant cocktail or with GPX+GSH. Incubation of cells for 10 days with ROS produced by xanthine oxidase/xanthine increased the level of PGC-1alpha, HKII and UCP3 mRNA by 175, 58 and 115%, respectively (p<0.05). A 10-day incubation of cells with antioxidants was found to have no effect on the basal mRNA content (p>0.05). The present data demonstrate that contraction of skeletal muscle cells leads to an enhanced formation of ROS and an elevation in PGC-1alpha, UCP3 and HKII mRNA content which is abolished in the presence of antioxidants, suggesting that ROS are of importance for the contraction induced increase in expression of these genes in skeletal muscle.

U2 - 10.1016/j.bbamcr.2006.06.010

DO - 10.1016/j.bbamcr.2006.06.010

M3 - Journal article

VL - 1763

SP - 969

EP - 976

JO - B B A - General Subjects

JF - B B A - General Subjects

SN - 0304-4165

IS - 9

ER -

ID: 314203