TY - ABST

T1 - Conjugated linoleic acid and I-carnitine combination effects on obesity related miRNAs in rats

AU - Jalili, Mahsa

PY - 2024

Y1 - 2024

N2 - Objectives: Obesity is a major global health issue, resulting in significant costs and increased mortality rates.Finding effective treatments for obesity is therefore essential. This study investigated the combined effects of LCarnitine (LC) and Conjugated Linoleic Acid (CLA) on weight loss and adipose tissue microRNA levels.Subjects /methods: Forty male Wistar rats weighing 150–200 g and about 8 weeks old were fed either a normal fatdiet (NFD) or a high-fat diet (HFD) for 8 weeks. Afterwards, the HFD group was randomly divided into foursubgroups: control, LC (200 mg kg− 1), CLA (500 mg kg− 1), and both (n = 8 in each group). The study lasted foran additional 4 weeks. The animals’ weights were recorded regularly, and after 12 weeks, miRNAs wereextracted from epididymal adipose tissue and analysed using real-time PCR. The miRNA expression levels of miR27a and miR-143 were compared between groups using Kolmogorov-Smirnov and one-way ANOVA tests in SPSSsoftware.Results: At the end of the first 8 weeks, the HFD group weighed significantly more than the NFD group. LCsignificantly decreased weight gain (4.2%) compared to the control group, whereas CLA alone (3.5%) or incombination with LC (3.1%) did not significantly slow weight gain. Real-time PCR results showed that the HFDgroup had higher miR-143 levels and lower miR-27a levels compared to the NFD group. LC and CLA increasedmiR-27a expression after 4 weeks, but their combination decreased miR-27a expression. CLA alone reduced miR143 expression, whereas LC had almost no effect. Their combination also reduced miR-143 expression.Conclusion: CLA and LC, which are considered weight loss supplements, can potentially regulate metabolism andcellular pathways. However, their combination did not show a synergistic effect on weight loss, possibly due tothe reduction in miR-27a expression. Further studies are needed to evaluate the effects of combined fat burnerson obesity treatment.

AB - Objectives: Obesity is a major global health issue, resulting in significant costs and increased mortality rates.Finding effective treatments for obesity is therefore essential. This study investigated the combined effects of LCarnitine (LC) and Conjugated Linoleic Acid (CLA) on weight loss and adipose tissue microRNA levels.Subjects /methods: Forty male Wistar rats weighing 150–200 g and about 8 weeks old were fed either a normal fatdiet (NFD) or a high-fat diet (HFD) for 8 weeks. Afterwards, the HFD group was randomly divided into foursubgroups: control, LC (200 mg kg− 1), CLA (500 mg kg− 1), and both (n = 8 in each group). The study lasted foran additional 4 weeks. The animals’ weights were recorded regularly, and after 12 weeks, miRNAs wereextracted from epididymal adipose tissue and analysed using real-time PCR. The miRNA expression levels of miR27a and miR-143 were compared between groups using Kolmogorov-Smirnov and one-way ANOVA tests in SPSSsoftware.Results: At the end of the first 8 weeks, the HFD group weighed significantly more than the NFD group. LCsignificantly decreased weight gain (4.2%) compared to the control group, whereas CLA alone (3.5%) or incombination with LC (3.1%) did not significantly slow weight gain. Real-time PCR results showed that the HFDgroup had higher miR-143 levels and lower miR-27a levels compared to the NFD group. LC and CLA increasedmiR-27a expression after 4 weeks, but their combination decreased miR-27a expression. CLA alone reduced miR143 expression, whereas LC had almost no effect. Their combination also reduced miR-143 expression.Conclusion: CLA and LC, which are considered weight loss supplements, can potentially regulate metabolism andcellular pathways. However, their combination did not show a synergistic effect on weight loss, possibly due tothe reduction in miR-27a expression. Further studies are needed to evaluate the effects of combined fat burnerson obesity treatment.

M3 - Conference abstract for conference

T2 - Metabolism Day

Y2 - 12 March 2024

ER -