The insulin-sensitizing effect of a single exercise bout is similar in type I and type II human muscle fibres

Research output: Contribution to journalJournal articlepeer-review

Standard

The insulin-sensitizing effect of a single exercise bout is similar in type I and type II human muscle fibres. / Larsen, Magnus Romme; Steenberg, Dorte Enggaard; Birk, Jesper Bratz; Sjøberg, Kim Anker; Kiens, Bente; Richter, Erik A.; Wojtaszewski, Jørgen.

In: Journal of Physiology, Vol. 598, No. 24, 2020, p. 5687-5699.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Larsen, MR, Steenberg, DE, Birk, JB, Sjøberg, KA, Kiens, B, Richter, EA & Wojtaszewski, J 2020, 'The insulin-sensitizing effect of a single exercise bout is similar in type I and type II human muscle fibres', Journal of Physiology, vol. 598, no. 24, pp. 5687-5699. https://doi.org/10.1113/JP280475

APA

Larsen, M. R., Steenberg, D. E., Birk, J. B., Sjøberg, K. A., Kiens, B., Richter, E. A., & Wojtaszewski, J. (2020). The insulin-sensitizing effect of a single exercise bout is similar in type I and type II human muscle fibres. Journal of Physiology, 598(24), 5687-5699. https://doi.org/10.1113/JP280475

Vancouver

Larsen MR, Steenberg DE, Birk JB, Sjøberg KA, Kiens B, Richter EA et al. The insulin-sensitizing effect of a single exercise bout is similar in type I and type II human muscle fibres. Journal of Physiology. 2020;598(24):5687-5699. https://doi.org/10.1113/JP280475

Author

Larsen, Magnus Romme ; Steenberg, Dorte Enggaard ; Birk, Jesper Bratz ; Sjøberg, Kim Anker ; Kiens, Bente ; Richter, Erik A. ; Wojtaszewski, Jørgen. / The insulin-sensitizing effect of a single exercise bout is similar in type I and type II human muscle fibres. In: Journal of Physiology. 2020 ; Vol. 598, No. 24. pp. 5687-5699.

Bibtex

@article{2344857ce4a64f36b70c6fd55f94ec3e,
title = "The insulin-sensitizing effect of a single exercise bout is similar in type I and type II human muscle fibres",
abstract = "Human skeletal muscle consists of slow-twitch (type I) and fast-twitch (type II) muscle fibres. Muscle insulin action, regulating glucose uptake and metabolism, is improved following a single exercise bout. Rodent studies suggest that this phenomenon is confined to specific muscle fibre types. Whether this phenomenon is also confined to specific fibre types in humans has not been described. To investigate this, nine healthy men underwent a euglycemic hyperinsulinemic clamp (EHC) in the recovery from a single bout of one-legged knee-extensor exercise. Pools of type I and type II fibres were prepared from muscle biopsies taken in the rested and prior exercised leg before and after theEHC. AMPK γ3 and TBC1D4 – two key proteins regulating muscle insulin action following exercise – were higher expressed in type II compared to type I fibres. However, phosphor-regulation of TBC1D4 was similar between fibre types when related to the total amount of TBC1D4 protein. The activating dephosphorylation of glycogen synthase was also similar in the two fibre types. Thus, insulin-induced regulation of key proteins important for transport and intracellular flux of glucose towards glycogen storage in the recovery from exercise, does not differ between fibre types. In conclusion, the insulinsensitizing effect of a single bout of exercise includes both type I and type II fibres in human skeletal muscle. This may be an important observation for future pharmacological strategies targeting muscle insulin sensitivity in humans.",
keywords = "Faculty of Science, Glucose metabolism, Insulin sensitivity, Insulin signalling, Muscle fibre type",
author = "Larsen, {Magnus Romme} and Steenberg, {Dorte Enggaard} and Birk, {Jesper Bratz} and Sj{\o}berg, {Kim Anker} and Bente Kiens and Richter, {Erik A.} and J{\o}rgen Wojtaszewski",
note = "CURIS 2020 NEXS 323",
year = "2020",
doi = "10.1113/JP280475",
language = "English",
volume = "598",
pages = "5687--5699",
journal = "The Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "24",

}

RIS

TY - JOUR

T1 - The insulin-sensitizing effect of a single exercise bout is similar in type I and type II human muscle fibres

AU - Larsen, Magnus Romme

AU - Steenberg, Dorte Enggaard

AU - Birk, Jesper Bratz

AU - Sjøberg, Kim Anker

AU - Kiens, Bente

AU - Richter, Erik A.

AU - Wojtaszewski, Jørgen

N1 - CURIS 2020 NEXS 323

PY - 2020

Y1 - 2020

N2 - Human skeletal muscle consists of slow-twitch (type I) and fast-twitch (type II) muscle fibres. Muscle insulin action, regulating glucose uptake and metabolism, is improved following a single exercise bout. Rodent studies suggest that this phenomenon is confined to specific muscle fibre types. Whether this phenomenon is also confined to specific fibre types in humans has not been described. To investigate this, nine healthy men underwent a euglycemic hyperinsulinemic clamp (EHC) in the recovery from a single bout of one-legged knee-extensor exercise. Pools of type I and type II fibres were prepared from muscle biopsies taken in the rested and prior exercised leg before and after theEHC. AMPK γ3 and TBC1D4 – two key proteins regulating muscle insulin action following exercise – were higher expressed in type II compared to type I fibres. However, phosphor-regulation of TBC1D4 was similar between fibre types when related to the total amount of TBC1D4 protein. The activating dephosphorylation of glycogen synthase was also similar in the two fibre types. Thus, insulin-induced regulation of key proteins important for transport and intracellular flux of glucose towards glycogen storage in the recovery from exercise, does not differ between fibre types. In conclusion, the insulinsensitizing effect of a single bout of exercise includes both type I and type II fibres in human skeletal muscle. This may be an important observation for future pharmacological strategies targeting muscle insulin sensitivity in humans.

AB - Human skeletal muscle consists of slow-twitch (type I) and fast-twitch (type II) muscle fibres. Muscle insulin action, regulating glucose uptake and metabolism, is improved following a single exercise bout. Rodent studies suggest that this phenomenon is confined to specific muscle fibre types. Whether this phenomenon is also confined to specific fibre types in humans has not been described. To investigate this, nine healthy men underwent a euglycemic hyperinsulinemic clamp (EHC) in the recovery from a single bout of one-legged knee-extensor exercise. Pools of type I and type II fibres were prepared from muscle biopsies taken in the rested and prior exercised leg before and after theEHC. AMPK γ3 and TBC1D4 – two key proteins regulating muscle insulin action following exercise – were higher expressed in type II compared to type I fibres. However, phosphor-regulation of TBC1D4 was similar between fibre types when related to the total amount of TBC1D4 protein. The activating dephosphorylation of glycogen synthase was also similar in the two fibre types. Thus, insulin-induced regulation of key proteins important for transport and intracellular flux of glucose towards glycogen storage in the recovery from exercise, does not differ between fibre types. In conclusion, the insulinsensitizing effect of a single bout of exercise includes both type I and type II fibres in human skeletal muscle. This may be an important observation for future pharmacological strategies targeting muscle insulin sensitivity in humans.

KW - Faculty of Science

KW - Glucose metabolism

KW - Insulin sensitivity

KW - Insulin signalling

KW - Muscle fibre type

U2 - 10.1113/JP280475

DO - 10.1113/JP280475

M3 - Journal article

C2 - 32916040

VL - 598

SP - 5687

EP - 5699

JO - The Journal of Physiology

JF - The Journal of Physiology

SN - 0022-3751

IS - 24

ER -

ID: 248547600