TY - JOUR

T1 - Rac1 and AMPK account for the majority of muscle glucose uptake stimulated by ex vivo contraction but not in vivo exercise

AU - Sylow, Lykke

AU - Møller, Lisbeth Liliendal Valbjørn

AU - Kleinert, Maximilian

AU - D'Hulst, Gommaar

AU - De Groote, Estelle

AU - Schjerling, Peter

AU - Steinberg, Gregory R

AU - Jensen, Thomas Elbenhardt

AU - Richter, Erik A.

N1 - CURIS 2017 NEXS 140

PY - 2017

Y1 - 2017

N2 - Exercise bypasses insulin resistance to increase glucose uptake in skeletal muscle and therefore represents an important alternative to stimulate glucose uptake in insulin resistant muscle. Both Rac1 and AMPK have been shown to partly regulate contraction-stimulated muscle glucose uptake but whether those two signaling pathways jointly account for the entire signal to glucose transport is unknown. We therefore studied the ability of contraction and exercise to stimulate glucose transport in isolated muscles with AMPK loss-of-function combined with either pharmacological inhibition or genetic deletion of Rac1.Muscle-specific knockout (mKO) of Rac1, a kinase dead α2 AMPK (α2KD), and double KO of β1 and β2 AMPK subunits (β1β2 KO), each partially decreased contraction-stimulated glucose transport in mouse soleus and EDL muscle. Interestingly, when pharmacological Rac1 inhibition was combined with either AMPK β1β2 KO or α2KD, contraction-stimulated glucose transport was almost completely inhibited. Importantly, α2KD+Rac1 mKO double-transgenic mice also displayed severely impaired contraction-stimulated glucose transport, while exercise-stimulated glucose uptake in vivo was only partially reduced by Rac1 mKO with no additive effect of α2KD. It is concluded that Rac1 and α2AMPK together account for almost the entire ex vivo contraction-response in muscle glucose transport, while only Rac1, but not α2AMPK, regulates muscle glucose uptake during submaximal exercise in vivo.

AB - Exercise bypasses insulin resistance to increase glucose uptake in skeletal muscle and therefore represents an important alternative to stimulate glucose uptake in insulin resistant muscle. Both Rac1 and AMPK have been shown to partly regulate contraction-stimulated muscle glucose uptake but whether those two signaling pathways jointly account for the entire signal to glucose transport is unknown. We therefore studied the ability of contraction and exercise to stimulate glucose transport in isolated muscles with AMPK loss-of-function combined with either pharmacological inhibition or genetic deletion of Rac1.Muscle-specific knockout (mKO) of Rac1, a kinase dead α2 AMPK (α2KD), and double KO of β1 and β2 AMPK subunits (β1β2 KO), each partially decreased contraction-stimulated glucose transport in mouse soleus and EDL muscle. Interestingly, when pharmacological Rac1 inhibition was combined with either AMPK β1β2 KO or α2KD, contraction-stimulated glucose transport was almost completely inhibited. Importantly, α2KD+Rac1 mKO double-transgenic mice also displayed severely impaired contraction-stimulated glucose transport, while exercise-stimulated glucose uptake in vivo was only partially reduced by Rac1 mKO with no additive effect of α2KD. It is concluded that Rac1 and α2AMPK together account for almost the entire ex vivo contraction-response in muscle glucose transport, while only Rac1, but not α2AMPK, regulates muscle glucose uptake during submaximal exercise in vivo.

KW - Journal Article

U2 - 10.2337/db16-1138

DO - 10.2337/db16-1138

M3 - Journal article

C2 - 28389470

VL - 66

SP - 1548

EP - 1559

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 6

ER -