Mechanisms limiting glycogen storage in muscle during prolonged insulin stimulation
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The extent to which muscle glycogen concentrations can be increased during exposure to maximal insulin concentrations and abundant glucose was investigated in the isolated perfused rat hindquarter preparation. Perfusion for 7 h in the presence of 20,000 microU/ml insulin and 11-13 mM glucose increased muscle glycogen concentrations to maximal values 2, 3, and 3.5 times above normal fed levels in fast-twitch white, slow-twitch red, and fast-twitch red fibers, respectively. Glucose uptake decreased (mean +/- SE) from 34.9 +/- 1.2 mumol.g-1.h-1 at 0 h to 7.5 +/- 0.7 after 7 h of perfusion. During the perfusion muscle glycogen synthase activity decreased and free intracellular glucose and glucose 6-phosphate increased indicating that glucose disposal was impaired. However, glucose transport as measured by the uptake of 3-O-[14C]methyl-D-glucose was also markedly decreased after 5 and 7 h of perfusion compared with initial values. Total muscle water concentration decreased during glycogen loading of the muscles. Mechanisms limiting glycogen storage under maximal insulin stimulation include impaired insulin-stimulated membrane transport of glucose as well as impaired intracellular glucose disposal.
|Journal||American Journal of Physiology (Consolidated)|
|Issue number||5 Pt 1|
|Number of pages||8|
|Publication status||Published - 1988|
- Adenosine Triphosphate, Animals, Glucose, Glucosephosphates, Glycogen, Glycogen Synthase, In Vitro Techniques, Insulin, Male, Muscles, Oxygen Consumption, Phosphocreatine, Rats, Rats, Inbred Strains, Stimulation, Chemical, Time Factors, Water