Glucose metabolism and metabolic flexibility in cultured skeletal muscle cells is related to exercise status in young male subjects

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Glucose metabolism and metabolic flexibility in cultured skeletal muscle cells is related to exercise status in young male subjects. / Lund, Jenny; S Tangen, Daniel; Wiig, Håvard; Stadheim, Hans K; Helle, Siw A; B Birk, Jesper; Ingemann-Hansen, Thorsten; Rustan, Arild C; Thoresen, G Hege; Wojtaszewski, Jørgen; T Kase, Eili; Jensen, Jørgen.

In: Archives of Physiology and Biochemistry, Vol. 124, No. 2, 2018, p. 119-130.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lund, J, S Tangen, D, Wiig, H, Stadheim, HK, Helle, SA, B Birk, J, Ingemann-Hansen, T, Rustan, AC, Thoresen, GH, Wojtaszewski, J, T Kase, E & Jensen, J 2018, 'Glucose metabolism and metabolic flexibility in cultured skeletal muscle cells is related to exercise status in young male subjects', Archives of Physiology and Biochemistry, vol. 124, no. 2, pp. 119-130. https://doi.org/10.1080/13813455.2017.1369547

APA

Lund, J., S Tangen, D., Wiig, H., Stadheim, H. K., Helle, S. A., B Birk, J., Ingemann-Hansen, T., Rustan, A. C., Thoresen, G. H., Wojtaszewski, J., T Kase, E., & Jensen, J. (2018). Glucose metabolism and metabolic flexibility in cultured skeletal muscle cells is related to exercise status in young male subjects. Archives of Physiology and Biochemistry, 124(2), 119-130. https://doi.org/10.1080/13813455.2017.1369547

Vancouver

Lund J, S Tangen D, Wiig H, Stadheim HK, Helle SA, B Birk J et al. Glucose metabolism and metabolic flexibility in cultured skeletal muscle cells is related to exercise status in young male subjects. Archives of Physiology and Biochemistry. 2018;124(2):119-130. https://doi.org/10.1080/13813455.2017.1369547

Author

Lund, Jenny ; S Tangen, Daniel ; Wiig, Håvard ; Stadheim, Hans K ; Helle, Siw A ; B Birk, Jesper ; Ingemann-Hansen, Thorsten ; Rustan, Arild C ; Thoresen, G Hege ; Wojtaszewski, Jørgen ; T Kase, Eili ; Jensen, Jørgen. / Glucose metabolism and metabolic flexibility in cultured skeletal muscle cells is related to exercise status in young male subjects. In: Archives of Physiology and Biochemistry. 2018 ; Vol. 124, No. 2. pp. 119-130.

Bibtex

@article{809d9f778dde42c5a49fddd08d4516c6,
title = "Glucose metabolism and metabolic flexibility in cultured skeletal muscle cells is related to exercise status in young male subjects",
abstract = "We hypothesised that skeletal muscles of healthy young people have a large variation in oxidative capacity and fibre-type composition, and aimed therefore to investigate glucose metabolism in biopsies and myotubes isolated from musculus vastus lateralis from healthy males with varying degrees of maximal oxygen uptake. Trained and intermediary trained subjects showed higher carbohydrate oxidation in vivo. Fibre-type distribution in biopsies and myotubes did not differ between groups. There was no correlation between fibre-type I expression in biopsies and myotubes. Myotubes from trained had higher deoxyglucose accumulation and fractional glucose oxidation (glucose oxidation relative to glucose uptake), and were also more sensitive to the suppressive action of acutely added oleic acid to the cells. Despite lack of correlation of fibre types between skeletal muscle biopsies and cultured cells, myotubes from trained subjects retained some of their phenotypes in vitro with respect to enhanced glucose metabolism and metabolic flexibility.",
keywords = "Training condition, Myotubes, Glucose, Suppression, Oxidation",
author = "Jenny Lund and {S Tangen}, Daniel and H{\aa}vard Wiig and Stadheim, {Hans K} and Helle, {Siw A} and {B Birk}, Jesper and Thorsten Ingemann-Hansen and Rustan, {Arild C} and Thoresen, {G Hege} and J{\o}rgen Wojtaszewski and {T Kase}, Eili and J{\o}rgen Jensen",
note = "CURIS 2018 NEXS 143",
year = "2018",
doi = "10.1080/13813455.2017.1369547",
language = "English",
volume = "124",
pages = "119--130",
journal = "Archives of Physiology and Biochemistry",
issn = "1381-3455",
publisher = "Taylor & Francis",
number = "2",

}

RIS

TY - JOUR

T1 - Glucose metabolism and metabolic flexibility in cultured skeletal muscle cells is related to exercise status in young male subjects

AU - Lund, Jenny

AU - S Tangen, Daniel

AU - Wiig, Håvard

AU - Stadheim, Hans K

AU - Helle, Siw A

AU - B Birk, Jesper

AU - Ingemann-Hansen, Thorsten

AU - Rustan, Arild C

AU - Thoresen, G Hege

AU - Wojtaszewski, Jørgen

AU - T Kase, Eili

AU - Jensen, Jørgen

N1 - CURIS 2018 NEXS 143

PY - 2018

Y1 - 2018

N2 - We hypothesised that skeletal muscles of healthy young people have a large variation in oxidative capacity and fibre-type composition, and aimed therefore to investigate glucose metabolism in biopsies and myotubes isolated from musculus vastus lateralis from healthy males with varying degrees of maximal oxygen uptake. Trained and intermediary trained subjects showed higher carbohydrate oxidation in vivo. Fibre-type distribution in biopsies and myotubes did not differ between groups. There was no correlation between fibre-type I expression in biopsies and myotubes. Myotubes from trained had higher deoxyglucose accumulation and fractional glucose oxidation (glucose oxidation relative to glucose uptake), and were also more sensitive to the suppressive action of acutely added oleic acid to the cells. Despite lack of correlation of fibre types between skeletal muscle biopsies and cultured cells, myotubes from trained subjects retained some of their phenotypes in vitro with respect to enhanced glucose metabolism and metabolic flexibility.

AB - We hypothesised that skeletal muscles of healthy young people have a large variation in oxidative capacity and fibre-type composition, and aimed therefore to investigate glucose metabolism in biopsies and myotubes isolated from musculus vastus lateralis from healthy males with varying degrees of maximal oxygen uptake. Trained and intermediary trained subjects showed higher carbohydrate oxidation in vivo. Fibre-type distribution in biopsies and myotubes did not differ between groups. There was no correlation between fibre-type I expression in biopsies and myotubes. Myotubes from trained had higher deoxyglucose accumulation and fractional glucose oxidation (glucose oxidation relative to glucose uptake), and were also more sensitive to the suppressive action of acutely added oleic acid to the cells. Despite lack of correlation of fibre types between skeletal muscle biopsies and cultured cells, myotubes from trained subjects retained some of their phenotypes in vitro with respect to enhanced glucose metabolism and metabolic flexibility.

KW - Training condition

KW - Myotubes

KW - Glucose

KW - Suppression

KW - Oxidation

U2 - 10.1080/13813455.2017.1369547

DO - 10.1080/13813455.2017.1369547

M3 - Journal article

C2 - 28862046

VL - 124

SP - 119

EP - 130

JO - Archives of Physiology and Biochemistry

JF - Archives of Physiology and Biochemistry

SN - 1381-3455

IS - 2

ER -

ID: 182825707