Exercise-induced increase in glucose transport, GLUT-4, and VAMP-2 in plasma membrane from human muscle

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A major effect of muscle contractions is an increase in sarcolemmal glucose transport. We have used a recently developed technique to produce sarcolemmal giant vesicles from human muscle biopsy samples obtained before and after exercise. Six men exercised for 10 min at 50% maximal O2 uptake (Vo2max) and then to fatigue at 100% Vo2max (5.7 +/- 0.2 min). Vesicle glucose transport at 5 mM increased from 3.3 +/- 0.6 pmol.microgram-1.min-1 at rest to 6.6 +/- 1.0 pmol.microgram-1.min-1 at fatigue (mean +/- SE, n = 6, P < 0.05). This increase in glucose transport was associated with a 1.6-fold increase in vesicle GLUT-4 protein content. Glucose transport normalized to GLUT-4 protein content also increased with exercise, suggesting increased intrinsic activity of GLUT-4. Furthermore, exercise resulted in a 1.4-fold increase in sarcolemmal vesicle-associated membrane protein (VAMP-2) content, suggesting that muscle contractions may induce trafficking of GLUT-4-containing vesicles via a mechanism similar to neurotransmitter release. Our results demonstrate for the first time exercise-induced translocation of GLUT-4 and VAMP-2 to the plasma membrane of human muscle and increased sarcolemmal glucose transport.

Original languageEnglish
JournalAmerican Journal of Physiology: Endocrinology and Metabolism
Issue number1
Pages (from-to)E197-E201
Number of pages5
Publication statusPublished - 1996

    Research areas

  • Adult, Biological Transport, Blotting, Western, Cell Membrane, Glucose, Glucose Transporter Type 4, Humans, Male, Membrane Proteins, Monosaccharide Transport Proteins, Muscle Proteins, Muscles, Physical Exertion, R-SNARE Proteins, Sarcolemma

ID: 154750965