Bioactivation of 2‐amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]‐pyridine by liver microsomes from three different rat strains

Research output: Contribution to journal › Journal article › Research › peer-review

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Bioactivation of 2‐amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]‐pyridine by liver microsomes from three different rat strains. / Dragsted, Lars Ove; Alexander, J; Wallin, H; Frandsen, H; Vang, O.

In: Pharmacology & Toxicology, Vol. 72, No. 6, 1993, p. 388-393.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Dragsted, LO, Alexander, J, Wallin, H, Frandsen, H & Vang, O 1993, 'Bioactivation of 2‐amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]‐pyridine by liver microsomes from three different rat strains', Pharmacology & Toxicology, vol. 72, no. 6, pp. 388-393. https://doi.org/10.1111/j.1600-0773.1993.tb01350.x

APA

Dragsted, L. O., Alexander, J., Wallin, H., Frandsen, H., & Vang, O. (1993). Bioactivation of 2‐amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]‐pyridine by liver microsomes from three different rat strains. Pharmacology & Toxicology, 72(6), 388-393. https://doi.org/10.1111/j.1600-0773.1993.tb01350.x

Vancouver

Dragsted LO, Alexander J, Wallin H, Frandsen H, Vang O. Bioactivation of 2‐amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]‐pyridine by liver microsomes from three different rat strains. Pharmacology & Toxicology. 1993;72(6):388-393. https://doi.org/10.1111/j.1600-0773.1993.tb01350.x

Author

Dragsted, Lars Ove ; Alexander, J ; Wallin, H ; Frandsen, H ; Vang, O. / Bioactivation of 2‐amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]‐pyridine by liver microsomes from three different rat strains. In: Pharmacology & Toxicology. 1993 ; Vol. 72, No. 6. pp. 388-393.

Bibtex

@article{4ab427d0f4dd42fe830484b4bb0e56e2,
title = "Bioactivation of 2‐amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]‐pyridine by liver microsomes from three different rat strains",
abstract = "The biotransformation of 2‐amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]‐pyridine (PhIP) and the protein binding of PhIP and 2‐amino‐3‐methylimidazo[4,5‐f]quinoline (IQ) was studied using microsomes from PCB‐pretreated or untreated male rats of the strains, Wistar, Fischer and Sprague‐Dawley. The microsomal monooxygenases, P450IA1 and IA2, which are important for the biotransformation of heterocyclic amines, were quantified by immunoblots. The two metabolites detected, 2‐amino‐1‐methyl‐6‐(4′‐hydroxyphenyl)imidazo[4,5‐b]pyridine (4′OH‐PhIP) and 2‐hydroxyamino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine (N2‐OH‐PhIP) were formed in similar amounts whereas no minor metabolites were found in our highly sensitive radiochemical assay. Irrespective of the rat strain used, pretreatment with PCB significantly induced both the activation and the detoxication in all three rat strains. Except for a significantly higher concentration of P450IA2 in microsomes from control and PCB induced Wistar rats, no major differences between the strains were found. 1993 Nordic Pharmacological Society",
author = "Dragsted, {Lars Ove} and J Alexander and H Wallin and H Frandsen and O Vang",
note = "(Ekstern)",
year = "1993",
doi = "10.1111/j.1600-0773.1993.tb01350.x",
language = "English",
volume = "72",
pages = "388--393",
journal = "Pharmacology and Toxicology",
issn = "0901-9928",
publisher = "Munksgaard ",
number = "6",

}

RIS

TY - JOUR

T1 - Bioactivation of 2‐amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]‐pyridine by liver microsomes from three different rat strains

AU - Dragsted, Lars Ove

AU - Alexander, J

AU - Wallin, H

AU - Frandsen, H

AU - Vang, O

N1 - (Ekstern)

PY - 1993

Y1 - 1993

N2 - The biotransformation of 2‐amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]‐pyridine (PhIP) and the protein binding of PhIP and 2‐amino‐3‐methylimidazo[4,5‐f]quinoline (IQ) was studied using microsomes from PCB‐pretreated or untreated male rats of the strains, Wistar, Fischer and Sprague‐Dawley. The microsomal monooxygenases, P450IA1 and IA2, which are important for the biotransformation of heterocyclic amines, were quantified by immunoblots. The two metabolites detected, 2‐amino‐1‐methyl‐6‐(4′‐hydroxyphenyl)imidazo[4,5‐b]pyridine (4′OH‐PhIP) and 2‐hydroxyamino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine (N2‐OH‐PhIP) were formed in similar amounts whereas no minor metabolites were found in our highly sensitive radiochemical assay. Irrespective of the rat strain used, pretreatment with PCB significantly induced both the activation and the detoxication in all three rat strains. Except for a significantly higher concentration of P450IA2 in microsomes from control and PCB induced Wistar rats, no major differences between the strains were found. 1993 Nordic Pharmacological Society

AB - The biotransformation of 2‐amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]‐pyridine (PhIP) and the protein binding of PhIP and 2‐amino‐3‐methylimidazo[4,5‐f]quinoline (IQ) was studied using microsomes from PCB‐pretreated or untreated male rats of the strains, Wistar, Fischer and Sprague‐Dawley. The microsomal monooxygenases, P450IA1 and IA2, which are important for the biotransformation of heterocyclic amines, were quantified by immunoblots. The two metabolites detected, 2‐amino‐1‐methyl‐6‐(4′‐hydroxyphenyl)imidazo[4,5‐b]pyridine (4′OH‐PhIP) and 2‐hydroxyamino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine (N2‐OH‐PhIP) were formed in similar amounts whereas no minor metabolites were found in our highly sensitive radiochemical assay. Irrespective of the rat strain used, pretreatment with PCB significantly induced both the activation and the detoxication in all three rat strains. Except for a significantly higher concentration of P450IA2 in microsomes from control and PCB induced Wistar rats, no major differences between the strains were found. 1993 Nordic Pharmacological Society

UR - http://www.scopus.com/inward/record.url?scp=0027288727&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0773.1993.tb01350.x

DO - 10.1111/j.1600-0773.1993.tb01350.x

M3 - Journal article

C2 - 8361949

AN - SCOPUS:0027288727

VL - 72

SP - 388

EP - 393

JO - Pharmacology and Toxicology

JF - Pharmacology and Toxicology

SN - 0901-9928

IS - 6

ER -

ID: 254779567