Column-switching high-performance liquid chromatographic assay for the determination of quercetin in human urine with ultraviolet absorbance detection

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Column-switching high-performance liquid chromatographic assay for the determination of quercetin in human urine with ultraviolet absorbance detection. / Nielsen, Salka E; Dragsted, Lars Ove.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 707, No. 1-2, 1998, p. 81-89.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nielsen, SE & Dragsted, LO 1998, 'Column-switching high-performance liquid chromatographic assay for the determination of quercetin in human urine with ultraviolet absorbance detection', Journal of Chromatography B: Biomedical Sciences and Applications, vol. 707, no. 1-2, pp. 81-89. https://doi.org/10.1016/S0378-4347(97)00574-4

APA

Nielsen, S. E., & Dragsted, L. O. (1998). Column-switching high-performance liquid chromatographic assay for the determination of quercetin in human urine with ultraviolet absorbance detection. Journal of Chromatography B: Biomedical Sciences and Applications, 707(1-2), 81-89. https://doi.org/10.1016/S0378-4347(97)00574-4

Vancouver

Nielsen SE, Dragsted LO. Column-switching high-performance liquid chromatographic assay for the determination of quercetin in human urine with ultraviolet absorbance detection. Journal of Chromatography B: Biomedical Sciences and Applications. 1998;707(1-2):81-89. https://doi.org/10.1016/S0378-4347(97)00574-4

Author

Nielsen, Salka E ; Dragsted, Lars Ove. / Column-switching high-performance liquid chromatographic assay for the determination of quercetin in human urine with ultraviolet absorbance detection. In: Journal of Chromatography B: Biomedical Sciences and Applications. 1998 ; Vol. 707, No. 1-2. pp. 81-89.

Bibtex

@article{2ddbd27d435849f6a463ed53b055f7b7,
title = "Column-switching high-performance liquid chromatographic assay for the determination of quercetin in human urine with ultraviolet absorbance detection",
abstract = "A high-performance liquid chromatographic method is described for the determination of quercetin in human urine using column-switching and ultraviolet (UV) absorbance detection. Urine samples were enzymatically hydrolysed and solid-phase extracted prior to injection onto the high- performance liquid chromatography (HPLC) system. Prior to elution of quercetin and the internal standard, fisetin, from the first column used for sample clean-up, the six-port valve was switched to the second column for analysis with UV detection. Detection of quercetin was accurate and reproducible, with a detection limit of 5 ng/ml. The method was applied to determine the urinary level of quercetin in 120 samples from an intervention study with fruit juice.",
keywords = "Quercetin",
author = "Nielsen, {Salka E} and Dragsted, {Lars Ove}",
note = "(Ekstern)",
year = "1998",
doi = "10.1016/S0378-4347(97)00574-4",
language = "English",
volume = "707",
pages = "81--89",
journal = "Journal of Chromatography - Biomedical Applications",
issn = "0378-4347",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Column-switching high-performance liquid chromatographic assay for the determination of quercetin in human urine with ultraviolet absorbance detection

AU - Nielsen, Salka E

AU - Dragsted, Lars Ove

N1 - (Ekstern)

PY - 1998

Y1 - 1998

N2 - A high-performance liquid chromatographic method is described for the determination of quercetin in human urine using column-switching and ultraviolet (UV) absorbance detection. Urine samples were enzymatically hydrolysed and solid-phase extracted prior to injection onto the high- performance liquid chromatography (HPLC) system. Prior to elution of quercetin and the internal standard, fisetin, from the first column used for sample clean-up, the six-port valve was switched to the second column for analysis with UV detection. Detection of quercetin was accurate and reproducible, with a detection limit of 5 ng/ml. The method was applied to determine the urinary level of quercetin in 120 samples from an intervention study with fruit juice.

AB - A high-performance liquid chromatographic method is described for the determination of quercetin in human urine using column-switching and ultraviolet (UV) absorbance detection. Urine samples were enzymatically hydrolysed and solid-phase extracted prior to injection onto the high- performance liquid chromatography (HPLC) system. Prior to elution of quercetin and the internal standard, fisetin, from the first column used for sample clean-up, the six-port valve was switched to the second column for analysis with UV detection. Detection of quercetin was accurate and reproducible, with a detection limit of 5 ng/ml. The method was applied to determine the urinary level of quercetin in 120 samples from an intervention study with fruit juice.

KW - Quercetin

UR - http://www.scopus.com/inward/record.url?scp=0032502861&partnerID=8YFLogxK

U2 - 10.1016/S0378-4347(97)00574-4

DO - 10.1016/S0378-4347(97)00574-4

M3 - Journal article

C2 - 9613936

AN - SCOPUS:0032502861

VL - 707

SP - 81

EP - 89

JO - Journal of Chromatography - Biomedical Applications

JF - Journal of Chromatography - Biomedical Applications

SN - 0378-4347

IS - 1-2

ER -

ID: 254770881