Effect of the methylenetetrahydrofolate reductase (MTHFR 677C>T) polymorphism on plasma homocysteine concentrations in healthy children is influenced by consumption of folate-fortified foods
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Effect of the methylenetetrahydrofolate reductase (MTHFR 677C>T) polymorphism on plasma homocysteine concentrations in healthy children is influenced by consumption of folate-fortified foods. / Papoutsakis, Constantina; Manios, Yannis; Magkos, Faidon; Papaconstantinou, Evaggelos; Schulpis, Kleopatra H; Zampelas, Antonis; Matalas, Antonia L; Yiannakouris, Nikos.
In: Nutrition, Vol. 26, No. 10, 2010, p. 969-974.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Effect of the methylenetetrahydrofolate reductase (MTHFR 677C>T) polymorphism on plasma homocysteine concentrations in healthy children is influenced by consumption of folate-fortified foods
AU - Papoutsakis, Constantina
AU - Manios, Yannis
AU - Magkos, Faidon
AU - Papaconstantinou, Evaggelos
AU - Schulpis, Kleopatra H
AU - Zampelas, Antonis
AU - Matalas, Antonia L
AU - Yiannakouris, Nikos
N1 - Copyright © 2010 Elsevier Inc. All rights reserved.
PY - 2010
Y1 - 2010
N2 - Objective: To explore the influence of folate-fortified foods (ready-to-eat [RTE] breakfast cereals or fruit-juice drinks) on the relation between the methylenetetrahydrofolate reductase (MTHFR 677C>T) polymorphism and plasma total homocysteine (tHcy) concentrations in healthy children.Methods: This was a cross-sectional study by face-to-face interview. A total of 186 sixth-grade students participated from randomly selected primary schools in Volos, Greece. Fasting plasma tHcy, folate, and vitamin B12 were measured. The MTHFR genotypes were determined. Anthropometric data were collected and dietary intake was assessed by two non-consecutive 24-h recalls. Participants were characterized as non-consumers of RTE breakfast cereals or fruit-juice drinks if there was no report of any such food during the 24-h recall interviews; all other children were classified as consumers.Results: Geometric means for plasma tHcy were higher, whereas plasma folate was lower in non-consumers compared with consumers. The sample was divided by consumption status (yes or no) to explore the significance of each polymorphism depending on consumption status. The association between the genotype and tHcy was restricted to non-consumers (P < 0.05). Specifically, only in children who did not consume RTE breakfast cereals or fruit-juice drinks did the TT genotype carriers exhibit higher tHcy concentrations when compared with C-allele carriers (P < 0.05). In contrast, in consumers, circulating tHcy was similar regardless of genotype.Conclusion: These observational findings support a beneficial effect of RTE breakfast cereals and fruit-juice drinks on lowering plasma tHcy and improving folate status in children. Also, consumption of folate-fortified foods modulates the association of the MTHFR 677C>T polymorphism with tHcy, suggesting that habitual consumption of folate-fortified foods is a practical approach in providing consistent protection to those children who may benefit the most, i.e., carriers of the TT genotype.
AB - Objective: To explore the influence of folate-fortified foods (ready-to-eat [RTE] breakfast cereals or fruit-juice drinks) on the relation between the methylenetetrahydrofolate reductase (MTHFR 677C>T) polymorphism and plasma total homocysteine (tHcy) concentrations in healthy children.Methods: This was a cross-sectional study by face-to-face interview. A total of 186 sixth-grade students participated from randomly selected primary schools in Volos, Greece. Fasting plasma tHcy, folate, and vitamin B12 were measured. The MTHFR genotypes were determined. Anthropometric data were collected and dietary intake was assessed by two non-consecutive 24-h recalls. Participants were characterized as non-consumers of RTE breakfast cereals or fruit-juice drinks if there was no report of any such food during the 24-h recall interviews; all other children were classified as consumers.Results: Geometric means for plasma tHcy were higher, whereas plasma folate was lower in non-consumers compared with consumers. The sample was divided by consumption status (yes or no) to explore the significance of each polymorphism depending on consumption status. The association between the genotype and tHcy was restricted to non-consumers (P < 0.05). Specifically, only in children who did not consume RTE breakfast cereals or fruit-juice drinks did the TT genotype carriers exhibit higher tHcy concentrations when compared with C-allele carriers (P < 0.05). In contrast, in consumers, circulating tHcy was similar regardless of genotype.Conclusion: These observational findings support a beneficial effect of RTE breakfast cereals and fruit-juice drinks on lowering plasma tHcy and improving folate status in children. Also, consumption of folate-fortified foods modulates the association of the MTHFR 677C>T polymorphism with tHcy, suggesting that habitual consumption of folate-fortified foods is a practical approach in providing consistent protection to those children who may benefit the most, i.e., carriers of the TT genotype.
KW - Beverages
KW - Child
KW - Cross-Sectional Studies
KW - Diet
KW - Edible Grain
KW - Female
KW - Folic Acid/administration & dosage
KW - Food, Fortified
KW - Fruit
KW - Genotype
KW - Greece
KW - Homocysteine/blood
KW - Humans
KW - Male
KW - Methylenetetrahydrofolate Reductase (NADPH2)/genetics
KW - Polymorphism, Genetic
KW - Vitamin B Complex/administration & dosage
U2 - 10.1016/j.nut.2009.09.022
DO - 10.1016/j.nut.2009.09.022
M3 - Journal article
C2 - 20097536
VL - 26
SP - 969
EP - 974
JO - Nutrition
JF - Nutrition
SN - 0899-9007
IS - 10
ER -
ID: 290670121