TY - JOUR

T1 - Analysis of human faecal host proteins: Responsiveness to 10-week dietary intervention modifying dietary protein intake in elderly males

AU - Gathercole, Jessica L

AU - Grosvenor, Anita J

AU - Lee, Erin

AU - Thomas, Ancy

AU - Mitchell, Cameron J

AU - Zeng, Nina

AU - D'Souza, Randall F

AU - Ramzan, Farha

AU - Sharma, Pankaja

AU - Knowles, Scott O

AU - Roy, Nicole C

AU - Sjödin, Anders

AU - Wagner, Karl-Heinz

AU - Milan, Amber M

AU - Mitchell, Sarah M

AU - Cameron-Smith, David

N1 - CURIS 2021 NEXS 062

PY - 2021

Y1 - 2021

N2 - Faecal proteomics targeting biomarkers of immunity and inflammation have demonstrated clinical application for the identification of changes in gastrointestinal function. However, there are limited comprehensive analyses of the host faecal proteome and how it may be influenced by dietary factors. To examine this, the Homo sapiens post-diet proteome of older males was analysed at the completion of a 10-week dietary intervention, either meeting the minimum dietary protein recommendations (RDA; n = 9) or twice the recommended dietary allowance (2RDA, n = 10). The host faecal proteome differed markedly between individuals, with only a small subset of proteins present in ≥ 60% of subjects (14 and 44 proteins, RDA and 2RDA, respectively, with only 7 common to both groups). No differences were observed between the diet groups on the profiles of host faecal proteins. Faecal proteins were detected from a wide range of protein classes, with high inter-individual variation and absence of obvious impact in response to diets with markedly different protein intake. This suggests that well-matched whole food diets with two-fold variation in protein intake maintained for 10 weeks have minimal impact on human faecal host proteins.

AB - Faecal proteomics targeting biomarkers of immunity and inflammation have demonstrated clinical application for the identification of changes in gastrointestinal function. However, there are limited comprehensive analyses of the host faecal proteome and how it may be influenced by dietary factors. To examine this, the Homo sapiens post-diet proteome of older males was analysed at the completion of a 10-week dietary intervention, either meeting the minimum dietary protein recommendations (RDA; n = 9) or twice the recommended dietary allowance (2RDA, n = 10). The host faecal proteome differed markedly between individuals, with only a small subset of proteins present in ≥ 60% of subjects (14 and 44 proteins, RDA and 2RDA, respectively, with only 7 common to both groups). No differences were observed between the diet groups on the profiles of host faecal proteins. Faecal proteins were detected from a wide range of protein classes, with high inter-individual variation and absence of obvious impact in response to diets with markedly different protein intake. This suggests that well-matched whole food diets with two-fold variation in protein intake maintained for 10 weeks have minimal impact on human faecal host proteins.

KW - Dietary protein

KW - Faeces

KW - Gastrointestinal health

KW - Host proteins

KW - Proteomics

UR - http://www.scopus.com/inward/record.url?scp=85100024454&partnerID=8YFLogxK

U2 - 10.3389/fnut.2020.595905

DO - 10.3389/fnut.2020.595905

M3 - Journal article

C2 - 33521034

AN - SCOPUS:85100024454

VL - 7

JO - Frontiers in Nutrition

JF - Frontiers in Nutrition

SN - 2296-861X

M1 - 595905

ER -