Pro- and anti-angiogenic factors in human skeletal muscle in response to acute exercise and training

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Standard

Pro- and anti-angiogenic factors in human skeletal muscle in response to acute exercise and training. / Høier, Birgitte; Nordsborg, Nikolai; Andersen, Søren; Jensen, Lasse Gliemann; Nybo, Lars; Bangsbo, Jens; Hellsten, Ylva.

I: Journal of Physiology, Bind 590, Nr. 3, 2012, s. 595-606.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Høier, B, Nordsborg, N, Andersen, S, Jensen, LG, Nybo, L, Bangsbo, J & Hellsten, Y 2012, 'Pro- and anti-angiogenic factors in human skeletal muscle in response to acute exercise and training', Journal of Physiology, bind 590, nr. 3, s. 595-606. https://doi.org/10.1113/jphysiol.2011.216135

APA

Høier, B., Nordsborg, N., Andersen, S., Jensen, L. G., Nybo, L., Bangsbo, J., & Hellsten, Y. (2012). Pro- and anti-angiogenic factors in human skeletal muscle in response to acute exercise and training. Journal of Physiology, 590(3), 595-606. https://doi.org/10.1113/jphysiol.2011.216135

Vancouver

Høier B, Nordsborg N, Andersen S, Jensen LG, Nybo L, Bangsbo J o.a. Pro- and anti-angiogenic factors in human skeletal muscle in response to acute exercise and training. Journal of Physiology. 2012;590(3):595-606. https://doi.org/10.1113/jphysiol.2011.216135

Author

Høier, Birgitte ; Nordsborg, Nikolai ; Andersen, Søren ; Jensen, Lasse Gliemann ; Nybo, Lars ; Bangsbo, Jens ; Hellsten, Ylva. / Pro- and anti-angiogenic factors in human skeletal muscle in response to acute exercise and training. I: Journal of Physiology. 2012 ; Bind 590, Nr. 3. s. 595-606.

Bibtex

@article{15251c9716904ceb9853b6810543cbac,
title = "Pro- and anti-angiogenic factors in human skeletal muscle in response to acute exercise and training",
abstract = "This study examined the effect of acute exercise and 4 weeks of aerobic training on skeletal muscle gene and protein expression of pro- and anti-angiogenic factors in 14 young male subjects. Training consisted of 60 min of cycling (~ 60{\%} of VO2 max), 3 times/week. Biopsies were obtained from m. v. lateralis before and after training. Muscle interstitial fluid was collected during cycling at week 0 and 4. Training increased (P <0.05) the capillary: fiber ratio and capillary density by 23{\%} and 12{\%}, respectively. The concentration of interstitial vascular endothelial growth factor (VEGF) in response to acute exercise increased similarly (>6-fold; P <0.05) before and after training. Resting protein levels of soluble VEGF receptor-1 in interstitial fluid, and of VEGF, Thrombospondin-1 (TSP-1) and Tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in muscle, were unaffected by training, whereas eNOS protein levels in muscle increased by 50{\%} (P <0.05). Before and after training, acute exercise induced a similar increase (P <0.05) in the mRNA level of Angiopoietin 2, matrix metalloproteinase 9 and TSP-1. After training, TIMP-1mRNA content increased with exercise (P <0.05). In conclusion, acute exercise induced a similar increase in the gene-expression of both pro- and anti-angiogenic factors in untrained and trained muscle. We propose that the increase in anti-angiogenic factors with exercise is important for modulation of angiogenesis. The lack of effect of training on basal muscle VEGF protein levels and VEGF secretion during exercise, suggests that increased VEGF levels are not a prerequisite for exercise induced capillary growth in healthy muscle.",
author = "Birgitte H{\o}ier and Nikolai Nordsborg and S{\o}ren Andersen and Jensen, {Lasse Gliemann} and Lars Nybo and Jens Bangsbo and Ylva Hellsten",
note = "CURIS 2012 5200 011",
year = "2012",
doi = "10.1113/jphysiol.2011.216135",
language = "English",
volume = "590",
pages = "595--606",
journal = "The Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "3",

}

RIS

TY - JOUR

T1 - Pro- and anti-angiogenic factors in human skeletal muscle in response to acute exercise and training

AU - Høier, Birgitte

AU - Nordsborg, Nikolai

AU - Andersen, Søren

AU - Jensen, Lasse Gliemann

AU - Nybo, Lars

AU - Bangsbo, Jens

AU - Hellsten, Ylva

N1 - CURIS 2012 5200 011

PY - 2012

Y1 - 2012

N2 - This study examined the effect of acute exercise and 4 weeks of aerobic training on skeletal muscle gene and protein expression of pro- and anti-angiogenic factors in 14 young male subjects. Training consisted of 60 min of cycling (~ 60% of VO2 max), 3 times/week. Biopsies were obtained from m. v. lateralis before and after training. Muscle interstitial fluid was collected during cycling at week 0 and 4. Training increased (P <0.05) the capillary: fiber ratio and capillary density by 23% and 12%, respectively. The concentration of interstitial vascular endothelial growth factor (VEGF) in response to acute exercise increased similarly (>6-fold; P <0.05) before and after training. Resting protein levels of soluble VEGF receptor-1 in interstitial fluid, and of VEGF, Thrombospondin-1 (TSP-1) and Tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in muscle, were unaffected by training, whereas eNOS protein levels in muscle increased by 50% (P <0.05). Before and after training, acute exercise induced a similar increase (P <0.05) in the mRNA level of Angiopoietin 2, matrix metalloproteinase 9 and TSP-1. After training, TIMP-1mRNA content increased with exercise (P <0.05). In conclusion, acute exercise induced a similar increase in the gene-expression of both pro- and anti-angiogenic factors in untrained and trained muscle. We propose that the increase in anti-angiogenic factors with exercise is important for modulation of angiogenesis. The lack of effect of training on basal muscle VEGF protein levels and VEGF secretion during exercise, suggests that increased VEGF levels are not a prerequisite for exercise induced capillary growth in healthy muscle.

AB - This study examined the effect of acute exercise and 4 weeks of aerobic training on skeletal muscle gene and protein expression of pro- and anti-angiogenic factors in 14 young male subjects. Training consisted of 60 min of cycling (~ 60% of VO2 max), 3 times/week. Biopsies were obtained from m. v. lateralis before and after training. Muscle interstitial fluid was collected during cycling at week 0 and 4. Training increased (P <0.05) the capillary: fiber ratio and capillary density by 23% and 12%, respectively. The concentration of interstitial vascular endothelial growth factor (VEGF) in response to acute exercise increased similarly (>6-fold; P <0.05) before and after training. Resting protein levels of soluble VEGF receptor-1 in interstitial fluid, and of VEGF, Thrombospondin-1 (TSP-1) and Tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in muscle, were unaffected by training, whereas eNOS protein levels in muscle increased by 50% (P <0.05). Before and after training, acute exercise induced a similar increase (P <0.05) in the mRNA level of Angiopoietin 2, matrix metalloproteinase 9 and TSP-1. After training, TIMP-1mRNA content increased with exercise (P <0.05). In conclusion, acute exercise induced a similar increase in the gene-expression of both pro- and anti-angiogenic factors in untrained and trained muscle. We propose that the increase in anti-angiogenic factors with exercise is important for modulation of angiogenesis. The lack of effect of training on basal muscle VEGF protein levels and VEGF secretion during exercise, suggests that increased VEGF levels are not a prerequisite for exercise induced capillary growth in healthy muscle.

U2 - 10.1113/jphysiol.2011.216135

DO - 10.1113/jphysiol.2011.216135

M3 - Journal article

C2 - 22155930

VL - 590

SP - 595

EP - 606

JO - The Journal of Physiology

JF - The Journal of Physiology

SN - 0022-3751

IS - 3

ER -

ID: 36089428