Kinase activity determination of specific AMPK complexes/heterotrimers in the skeletal muscle

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningfagfællebedømt

Measuring the kinase activity of the 5'-AMP-activated protein kinase (AMPK) is an essential part of understanding the regulation of this metabolic master switch. The AMPK heterotrimer can exist in 12 different constellations with potentially diverse activation patterns. It is therefore important to be able to measure heterotrimer-specific activity to discriminate between these patterns. In this chapter we describe how to measure the AMPK activity of specific heterotrimeric complexes by consecutive immunoprecipitations and how the assay can be performed in a medium throughput fashion using 96-well plates.

OriginalsprogEngelsk
TitelAMPK : Methods and Protecols
RedaktørerDietbert Neumann, Benoit Viollet
Antal sider14
Udgivelses stedNew York
ForlagHumana Press
Publikationsdato2018
Sider215-228
Kapitel14
ISBN (Trykt)978-1-4939-7597-6
ISBN (Elektronisk)978-1-4939-7598-3
DOI
StatusUdgivet - 2018
NavnMethods in Molecular Biology
Nummer1732
ISSN1064-3745

Bibliografisk note

CURIS 2018 NEXS 080

ID: 191294168