Identifying the heterotrimeric complex stoichiometry of AMPK in skeletal muscle by immunoprecipitation

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningfagfællebedømt

The 5'-AMP-activated protein kinase is a complicated enzyme consisting of three different subunits, each of which is expressed as two or three isoforms. This gives the possibility of 12 different heterotrimeric complexes, which could have diverse functions within the cell. To map out which of these complexes are present and to what extent in skeletal muscle, we have used the immunoprecipitation technique and analyzed both the precipitates and the remaining supernatants for coprecipitation of complex partners. We have fine-tuned this method to give the best results on lysates from the skeletal muscle, liver, and heart muscle from mouse to man.

OriginalsprogEngelsk
TitelAMPK : Methods and Protecols
RedaktørerDietbert Neumann, Benoit Viollet
Antal sider11
Udgivelses stedNew York
ForlagHumana Press
Publikationsdato2018
Sider203-213
Kapitel13
ISBN (Trykt)978-1-4939-7597-6
ISBN (Elektronisk)978-1-4939-7598-3
DOI
StatusUdgivet - 2018
NavnMethods in Molecular Biology
Nummer1732
ISSN1064-3745

Bibliografisk note

CURIS 2018 NEXS 079

ID: 191293422