Enhanced insulin signaling in human skeletal muscle and adipose tissue following gastric bypass surgery

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Standard

Enhanced insulin signaling in human skeletal muscle and adipose tissue following gastric bypass surgery. / Albers, Peter Hjorth; Bojsen-Moller, Kirstine N; Dirksen, Carsten; Serup, Annette Karen; Kristensen, Dorte Enggaard; Frystyk, Jan; Clausen, Trine R; Kiens, Bente; Richter, Erik A.; Madsbad, Sten; Wojtaszewski, Jørgen.

I: American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, Bind 309, Nr. 5, 2015, s. R510-R524.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Albers, PH, Bojsen-Moller, KN, Dirksen, C, Serup, AK, Kristensen, DE, Frystyk, J, Clausen, TR, Kiens, B, Richter, EA, Madsbad, S & Wojtaszewski, J 2015, 'Enhanced insulin signaling in human skeletal muscle and adipose tissue following gastric bypass surgery', American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, bind 309, nr. 5, s. R510-R524. https://doi.org/10.1152/ajpregu.00228.2014

APA

Albers, P. H., Bojsen-Moller, K. N., Dirksen, C., Serup, A. K., Kristensen, D. E., Frystyk, J., ... Wojtaszewski, J. (2015). Enhanced insulin signaling in human skeletal muscle and adipose tissue following gastric bypass surgery. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, 309(5), R510-R524. https://doi.org/10.1152/ajpregu.00228.2014

Vancouver

Albers PH, Bojsen-Moller KN, Dirksen C, Serup AK, Kristensen DE, Frystyk J o.a. Enhanced insulin signaling in human skeletal muscle and adipose tissue following gastric bypass surgery. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology. 2015;309(5):R510-R524. https://doi.org/10.1152/ajpregu.00228.2014

Author

Albers, Peter Hjorth ; Bojsen-Moller, Kirstine N ; Dirksen, Carsten ; Serup, Annette Karen ; Kristensen, Dorte Enggaard ; Frystyk, Jan ; Clausen, Trine R ; Kiens, Bente ; Richter, Erik A. ; Madsbad, Sten ; Wojtaszewski, Jørgen. / Enhanced insulin signaling in human skeletal muscle and adipose tissue following gastric bypass surgery. I: American Journal of Physiology: Regulatory, Integrative and Comparative Physiology. 2015 ; Bind 309, Nr. 5. s. R510-R524.

Bibtex

@article{69bc91ae68b048d79a6914512c90331c,
title = "Enhanced insulin signaling in human skeletal muscle and adipose tissue following gastric bypass surgery",
abstract = "Roux-en-Y gastric bypass (RYGB) leads to increased peripheral insulin sensitivity. The aim of this study was to investigate the effect of RYGB on expression and regulation of proteins involved in regulation of peripheral glucose metabolism. Skeletal muscle and adipose tissue biopsies from glucose tolerant and type 2 diabetic subjects at fasting and during a hyperinsulinemic-euglycemic clamp before as well as 1 week, 3 and 12 months after RYGB were analyzed for relevant insulin effector proteins/signaling components. Improvement in peripheral insulin sensitivity mainly occurred at 12 months post-surgery when major weight loss was evident and occurred concomitantly with alterations in plasma adiponectin and in protein expression/signaling in peripheral tissues. In skeletal muscle, protein expression of GLUT4, phosphorylated levels of TBC1D4 as well as insulin-induced changes in phosphorylation of Akt and glycogen synthase activity were enhanced 12 months post-surgery. In adipose tissue, protein expression of GLUT4, Akt2, TBC1D4 and acetyl-CoA carboxylase (ACC), phosphorylated levels of AMP-activated protein kinase and ACC as well as insulin-induced changes in phosphorylation of Akt and TBC1D4 were enhanced 12 months post-surgery. Adipose tissue from glucose tolerant subjects was the most responsive to RYGB compared to type 2 diabetic patients, whereas changes in skeletal muscle were largely similar in these two groups. In conclusion, an improved molecular insulin sensitive phenotype of skeletal muscle and adipose tissue appears to contribute to the improved whole body insulin action following a substantial weight loss after RYGB.",
author = "Albers, {Peter Hjorth} and Bojsen-Moller, {Kirstine N} and Carsten Dirksen and Serup, {Annette Karen} and Kristensen, {Dorte Enggaard} and Jan Frystyk and Clausen, {Trine R} and Bente Kiens and Richter, {Erik A.} and Sten Madsbad and J{\o}rgen Wojtaszewski",
note = "CURIS 2015 NEXS 314",
year = "2015",
doi = "10.1152/ajpregu.00228.2014",
language = "English",
volume = "309",
pages = "R510--R524",
journal = "American Journal of Physiology: Regulatory, Integrative and Comparative Physiology",
issn = "0363-6119",
publisher = "American Physiological Society",
number = "5",

}

RIS

TY - JOUR

T1 - Enhanced insulin signaling in human skeletal muscle and adipose tissue following gastric bypass surgery

AU - Albers, Peter Hjorth

AU - Bojsen-Moller, Kirstine N

AU - Dirksen, Carsten

AU - Serup, Annette Karen

AU - Kristensen, Dorte Enggaard

AU - Frystyk, Jan

AU - Clausen, Trine R

AU - Kiens, Bente

AU - Richter, Erik A.

AU - Madsbad, Sten

AU - Wojtaszewski, Jørgen

N1 - CURIS 2015 NEXS 314

PY - 2015

Y1 - 2015

N2 - Roux-en-Y gastric bypass (RYGB) leads to increased peripheral insulin sensitivity. The aim of this study was to investigate the effect of RYGB on expression and regulation of proteins involved in regulation of peripheral glucose metabolism. Skeletal muscle and adipose tissue biopsies from glucose tolerant and type 2 diabetic subjects at fasting and during a hyperinsulinemic-euglycemic clamp before as well as 1 week, 3 and 12 months after RYGB were analyzed for relevant insulin effector proteins/signaling components. Improvement in peripheral insulin sensitivity mainly occurred at 12 months post-surgery when major weight loss was evident and occurred concomitantly with alterations in plasma adiponectin and in protein expression/signaling in peripheral tissues. In skeletal muscle, protein expression of GLUT4, phosphorylated levels of TBC1D4 as well as insulin-induced changes in phosphorylation of Akt and glycogen synthase activity were enhanced 12 months post-surgery. In adipose tissue, protein expression of GLUT4, Akt2, TBC1D4 and acetyl-CoA carboxylase (ACC), phosphorylated levels of AMP-activated protein kinase and ACC as well as insulin-induced changes in phosphorylation of Akt and TBC1D4 were enhanced 12 months post-surgery. Adipose tissue from glucose tolerant subjects was the most responsive to RYGB compared to type 2 diabetic patients, whereas changes in skeletal muscle were largely similar in these two groups. In conclusion, an improved molecular insulin sensitive phenotype of skeletal muscle and adipose tissue appears to contribute to the improved whole body insulin action following a substantial weight loss after RYGB.

AB - Roux-en-Y gastric bypass (RYGB) leads to increased peripheral insulin sensitivity. The aim of this study was to investigate the effect of RYGB on expression and regulation of proteins involved in regulation of peripheral glucose metabolism. Skeletal muscle and adipose tissue biopsies from glucose tolerant and type 2 diabetic subjects at fasting and during a hyperinsulinemic-euglycemic clamp before as well as 1 week, 3 and 12 months after RYGB were analyzed for relevant insulin effector proteins/signaling components. Improvement in peripheral insulin sensitivity mainly occurred at 12 months post-surgery when major weight loss was evident and occurred concomitantly with alterations in plasma adiponectin and in protein expression/signaling in peripheral tissues. In skeletal muscle, protein expression of GLUT4, phosphorylated levels of TBC1D4 as well as insulin-induced changes in phosphorylation of Akt and glycogen synthase activity were enhanced 12 months post-surgery. In adipose tissue, protein expression of GLUT4, Akt2, TBC1D4 and acetyl-CoA carboxylase (ACC), phosphorylated levels of AMP-activated protein kinase and ACC as well as insulin-induced changes in phosphorylation of Akt and TBC1D4 were enhanced 12 months post-surgery. Adipose tissue from glucose tolerant subjects was the most responsive to RYGB compared to type 2 diabetic patients, whereas changes in skeletal muscle were largely similar in these two groups. In conclusion, an improved molecular insulin sensitive phenotype of skeletal muscle and adipose tissue appears to contribute to the improved whole body insulin action following a substantial weight loss after RYGB.

U2 - 10.1152/ajpregu.00228.2014

DO - 10.1152/ajpregu.00228.2014

M3 - Journal article

C2 - 26062634

VL - 309

SP - R510-R524

JO - American Journal of Physiology: Regulatory, Integrative and Comparative Physiology

JF - American Journal of Physiology: Regulatory, Integrative and Comparative Physiology

SN - 0363-6119

IS - 5

ER -

ID: 142062356