TY - JOUR

T1 - Restitution of gut microbiota in Ugandan children administered with probiotics (Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis BB-12) during treatment for severe acute malnutrition

AU - Castro-Mejía, Josué L.

AU - O’Ferrall, Sinéad

AU - Krych, Łukasz

AU - O’Mahony, Elaine

AU - Namusoke, Hanifa

AU - Lanyero, Betty

AU - Kot, Witold

AU - Nabukeera-Barungi, Nicolette

AU - Michaelsen, Kim Fleischer

AU - Mølgaard, Christian

AU - Friis, Henrik

AU - Grenov, Benedikte

AU - Nielsen, Dennis S.

N1 - CURIS 2020 NEXS 071

PY - 2020

Y1 - 2020

N2 - Severe acute malnutrition (SAM) is a major challenge in low-income countries and gut microbiota (GM) dysbiosis may play a role in its etiology. Here, we determined the GM evolution during rehabilitation from SAM and the impact of probiotics (Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis BB-12) supplementation. The GM (16S rRNA gene amplicon sequencing) of children admitted to hospital with SAM showed distinct composition over admission (e.g. Klebsiella spp., and Enterobacteriaceae spp.), discharge (e.g. Clostridiaceae spp., Veilonella dispar) and follow-up (e.g. Lactobacillus ruminis, Blautia spp., Faecalibacterium prausnitzii), reaching similar β- and α-diversity as healthy individuals. Children with diarrhea had reduced distribution of Bacteroidaceae, Lachnospiraceae, increased Enterobacteriaceae and Moraxellaceae, and lower α-diversity. Children suffering from edematous SAM had diminished proportion of Prevotellaceae, Lachnospiraceae, Ruminoccaceae and a higher α-diversity when compared to non-edematous SAM. Supplementation of probiotics did not influence β-diversity upon discharge or follow-up, but it increased (p <.05) the number of observed species [SE: > 4.5]. Children where the probiotic species were detected had lower cumulative incidence (p <.001) of diarrhea during the follow-up period compared to children receiving placebo and children receiving probiotics, but where the probiotics were not detected. The GM of children with non-edematous and edematous SAM differ in composition, which might have implications for future GM targeted treatments. Probiotics treatment reduced the cumulative incidence of diarrhea during the outpatient phase, with the strongest effect in children where the administered probiotics could be detected in the GM.

AB - Severe acute malnutrition (SAM) is a major challenge in low-income countries and gut microbiota (GM) dysbiosis may play a role in its etiology. Here, we determined the GM evolution during rehabilitation from SAM and the impact of probiotics (Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis BB-12) supplementation. The GM (16S rRNA gene amplicon sequencing) of children admitted to hospital with SAM showed distinct composition over admission (e.g. Klebsiella spp., and Enterobacteriaceae spp.), discharge (e.g. Clostridiaceae spp., Veilonella dispar) and follow-up (e.g. Lactobacillus ruminis, Blautia spp., Faecalibacterium prausnitzii), reaching similar β- and α-diversity as healthy individuals. Children with diarrhea had reduced distribution of Bacteroidaceae, Lachnospiraceae, increased Enterobacteriaceae and Moraxellaceae, and lower α-diversity. Children suffering from edematous SAM had diminished proportion of Prevotellaceae, Lachnospiraceae, Ruminoccaceae and a higher α-diversity when compared to non-edematous SAM. Supplementation of probiotics did not influence β-diversity upon discharge or follow-up, but it increased (p <.05) the number of observed species [SE: > 4.5]. Children where the probiotic species were detected had lower cumulative incidence (p <.001) of diarrhea during the follow-up period compared to children receiving placebo and children receiving probiotics, but where the probiotics were not detected. The GM of children with non-edematous and edematous SAM differ in composition, which might have implications for future GM targeted treatments. Probiotics treatment reduced the cumulative incidence of diarrhea during the outpatient phase, with the strongest effect in children where the administered probiotics could be detected in the GM.

KW - Bifidobacterium animalis subsp. Lactis BB-12

KW - gut microbiota

KW - Lactobacillus rhamnosus GG

KW - probiotics

KW - restitution

KW - Severe acute malnutrition treatment

U2 - 10.1080/19490976.2020.1712982

DO - 10.1080/19490976.2020.1712982

M3 - Journal article

C2 - 31959047

AN - SCOPUS:85078443759

VL - 11

SP - 855

EP - 867

JO - Gut Microbes

JF - Gut Microbes

SN - 1949-0976

IS - 4

ER -