Integrated method for quantitative morphometry and oxygen transport modeling in striated muscle

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Standard

Integrated method for quantitative morphometry and oxygen transport modeling in striated muscle. / Al-Shammari, Abdullah A; Kissane, Roger W P; Holbek, Simon; Mackey, Abigail Louise; Andersen, Thomas Rostgaard; Gaffney, Eamonn A; Kjær, Michael; Egginton, Stuart.

I: Journal of Applied Physiology, Bind 126, Nr. 3, 2019, s. 544-557.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Al-Shammari, AA, Kissane, RWP, Holbek, S, Mackey, AL, Andersen, TR, Gaffney, EA, Kjær, M & Egginton, S 2019, 'Integrated method for quantitative morphometry and oxygen transport modeling in striated muscle', Journal of Applied Physiology, bind 126, nr. 3, s. 544-557. https://doi.org/10.1152/japplphysiol.00170.2018

APA

Al-Shammari, A. A., Kissane, R. W. P., Holbek, S., Mackey, A. L., Andersen, T. R., Gaffney, E. A., Kjær, M., & Egginton, S. (2019). Integrated method for quantitative morphometry and oxygen transport modeling in striated muscle. Journal of Applied Physiology, 126(3), 544-557. https://doi.org/10.1152/japplphysiol.00170.2018

Vancouver

Al-Shammari AA, Kissane RWP, Holbek S, Mackey AL, Andersen TR, Gaffney EA o.a. Integrated method for quantitative morphometry and oxygen transport modeling in striated muscle. Journal of Applied Physiology. 2019;126(3):544-557. https://doi.org/10.1152/japplphysiol.00170.2018

Author

Al-Shammari, Abdullah A ; Kissane, Roger W P ; Holbek, Simon ; Mackey, Abigail Louise ; Andersen, Thomas Rostgaard ; Gaffney, Eamonn A ; Kjær, Michael ; Egginton, Stuart. / Integrated method for quantitative morphometry and oxygen transport modeling in striated muscle. I: Journal of Applied Physiology. 2019 ; Bind 126, Nr. 3. s. 544-557.

Bibtex

@article{04f00b9a59fa4e2e80d26e4cd9ab891c,
title = "Integrated method for quantitative morphometry and oxygen transport modeling in striated muscle",
abstract = "Identifying structural limitations in O2 transport is primarily restricted by current methods employed to characterise the nature of physiological remodelling. Inadequate resolution or breadth of available data has impaired development of routine diagnostic protocols and effective therapeutic strategies. Understanding O2 transport within striated muscle faces major challenges, most notably in quantifying how well individual fibres are supplied by the microcirculation, which has necessitated exploring tissue O2 supply using theoretical modelling of diffusive exchange. Having identified capillary domains as a suitable model for the description of local O2 supply, and requiring less computation than numerically calculating the trapping regions that are supplied by each capillary via biophysical transport models, we sought to design a high throughput method for histological analysis. We present an integrated package that identifies optimal protocols for identification of important input elements, processing of digitised images with semi-automated routines, and incorporation of these data into a mathematical modelling framework with computed output visualised as the tissue partial pressure of O2 (PO2) distribution across a biopsy sample. Worked examples are provided using muscle samples from experiments involving rats and humans.",
keywords = "Faculty of Science, Mathematical modeling, Skeletal muscle, Image analysis, Fiber type, Capillary supply, DTect",
author = "Al-Shammari, {Abdullah A} and Kissane, {Roger W P} and Simon Holbek and Mackey, {Abigail Louise} and Andersen, {Thomas Rostgaard} and Gaffney, {Eamonn A} and Michael Kj{\ae}r and Stuart Egginton",
note = "CURIS 2019 NEXS 084",
year = "2019",
doi = "10.1152/japplphysiol.00170.2018",
language = "English",
volume = "126",
pages = "544--557",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "3",

}

RIS

TY - JOUR

T1 - Integrated method for quantitative morphometry and oxygen transport modeling in striated muscle

AU - Al-Shammari, Abdullah A

AU - Kissane, Roger W P

AU - Holbek, Simon

AU - Mackey, Abigail Louise

AU - Andersen, Thomas Rostgaard

AU - Gaffney, Eamonn A

AU - Kjær, Michael

AU - Egginton, Stuart

N1 - CURIS 2019 NEXS 084

PY - 2019

Y1 - 2019

N2 - Identifying structural limitations in O2 transport is primarily restricted by current methods employed to characterise the nature of physiological remodelling. Inadequate resolution or breadth of available data has impaired development of routine diagnostic protocols and effective therapeutic strategies. Understanding O2 transport within striated muscle faces major challenges, most notably in quantifying how well individual fibres are supplied by the microcirculation, which has necessitated exploring tissue O2 supply using theoretical modelling of diffusive exchange. Having identified capillary domains as a suitable model for the description of local O2 supply, and requiring less computation than numerically calculating the trapping regions that are supplied by each capillary via biophysical transport models, we sought to design a high throughput method for histological analysis. We present an integrated package that identifies optimal protocols for identification of important input elements, processing of digitised images with semi-automated routines, and incorporation of these data into a mathematical modelling framework with computed output visualised as the tissue partial pressure of O2 (PO2) distribution across a biopsy sample. Worked examples are provided using muscle samples from experiments involving rats and humans.

AB - Identifying structural limitations in O2 transport is primarily restricted by current methods employed to characterise the nature of physiological remodelling. Inadequate resolution or breadth of available data has impaired development of routine diagnostic protocols and effective therapeutic strategies. Understanding O2 transport within striated muscle faces major challenges, most notably in quantifying how well individual fibres are supplied by the microcirculation, which has necessitated exploring tissue O2 supply using theoretical modelling of diffusive exchange. Having identified capillary domains as a suitable model for the description of local O2 supply, and requiring less computation than numerically calculating the trapping regions that are supplied by each capillary via biophysical transport models, we sought to design a high throughput method for histological analysis. We present an integrated package that identifies optimal protocols for identification of important input elements, processing of digitised images with semi-automated routines, and incorporation of these data into a mathematical modelling framework with computed output visualised as the tissue partial pressure of O2 (PO2) distribution across a biopsy sample. Worked examples are provided using muscle samples from experiments involving rats and humans.

KW - Faculty of Science

KW - Mathematical modeling

KW - Skeletal muscle

KW - Image analysis

KW - Fiber type

KW - Capillary supply

KW - DTect

U2 - 10.1152/japplphysiol.00170.2018

DO - 10.1152/japplphysiol.00170.2018

M3 - Journal article

C2 - 30521427

VL - 126

SP - 544

EP - 557

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 3

ER -

ID: 209676700