Human muscular mitochondrial fusion in athletes during exercise

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

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Human muscular mitochondrial fusion in athletes during exercise. / Huertas, Jesús R; Ruiz-Ojeda, Francisco Javier; Plaza-Díaz, Julio; Nordsborg, Nikolai Baastrup; Martín-Albo, Jesús; Rueda-Robles, Ascensión; Casuso, Rafael A.

I: F A S E B Journal, Bind 33, Nr. 11, 2019, s. 12087-12098.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Huertas, JR, Ruiz-Ojeda, FJ, Plaza-Díaz, J, Nordsborg, NB, Martín-Albo, J, Rueda-Robles, A & Casuso, RA 2019, 'Human muscular mitochondrial fusion in athletes during exercise', F A S E B Journal, bind 33, nr. 11, s. 12087-12098. https://doi.org/10.1096/fj.201900365RR

APA

Huertas, J. R., Ruiz-Ojeda, F. J., Plaza-Díaz, J., Nordsborg, N. B., Martín-Albo, J., Rueda-Robles, A., & Casuso, R. A. (2019). Human muscular mitochondrial fusion in athletes during exercise. F A S E B Journal, 33(11), 12087-12098. https://doi.org/10.1096/fj.201900365RR

Vancouver

Huertas JR, Ruiz-Ojeda FJ, Plaza-Díaz J, Nordsborg NB, Martín-Albo J, Rueda-Robles A o.a. Human muscular mitochondrial fusion in athletes during exercise. F A S E B Journal. 2019;33(11):12087-12098. https://doi.org/10.1096/fj.201900365RR

Author

Huertas, Jesús R ; Ruiz-Ojeda, Francisco Javier ; Plaza-Díaz, Julio ; Nordsborg, Nikolai Baastrup ; Martín-Albo, Jesús ; Rueda-Robles, Ascensión ; Casuso, Rafael A. / Human muscular mitochondrial fusion in athletes during exercise. I: F A S E B Journal. 2019 ; Bind 33, Nr. 11. s. 12087-12098.

Bibtex

@article{ed5746a7f56943c6bbe64ac35db2c621,
title = "Human muscular mitochondrial fusion in athletes during exercise",
abstract = "The main objective of this work was to investigate whether mitochondrial fusion occurs in the skeletal muscle of well-trained athletes in response to high-intensity exercise. Well-trained swimmers (n = 9) performed a duration-matched sprint interval training (SIT) and high-intensity high-volume training (HIHVT) session on separate days. Muscle samples from M. triceps brachii were taken before, immediately after, and 3 h after the training sessions. Transmission electron microscopy (TEM) was applied to assess mitochondrial morphology. Moreover, expression of genes coding for regulators of mitochondrial fusion and fission were assessed by real-time quantitative PCR. In addition, mitofusin (MFN)2 and optic atrophy 1 (OPA1) were quantified by Western blot analysis. TEM analyses showed that mitochondrial morphology remained altered for 3 h after HIHVT, whereas SIT-induced changes were only evident immediately after exercise. Only SIT increased MFN1 and MFN2 mRNA expression, whereas SIT and HIHVT both increased MFN2 protein content 3 h after exercise. Notably, only HIHVT increased OPA1 protein content. Mitochondrial morphologic changes that suggest fusion occurs in well-adapted athletes during exercise. However, HIHVT appears as a more robust inducer of mitochondrial fusion events than SIT. Indeed, SIT induces a rapid and transient change in mitochondrial morphology. ",
keywords = "Faculty of Science, Physical activity, Mitochondrial fission, Skeletal muscle, Mitofusin 2",
author = "Huertas, {Jes{\'u}s R} and Ruiz-Ojeda, {Francisco Javier} and Julio Plaza-D{\'i}az and Nordsborg, {Nikolai Baastrup} and Jes{\'u}s Mart{\'i}n-Albo and Ascensi{\'o}n Rueda-Robles and Casuso, {Rafael A}",
note = "CURIS 2019 NEXS 260",
year = "2019",
doi = "10.1096/fj.201900365RR",
language = "English",
volume = "33",
pages = "12087--12098",
journal = "F A S E B Journal",
issn = "0892-6638",
publisher = "Federation of American Societies for Experimental Biology",
number = "11",

}

RIS

TY - JOUR

T1 - Human muscular mitochondrial fusion in athletes during exercise

AU - Huertas, Jesús R

AU - Ruiz-Ojeda, Francisco Javier

AU - Plaza-Díaz, Julio

AU - Nordsborg, Nikolai Baastrup

AU - Martín-Albo, Jesús

AU - Rueda-Robles, Ascensión

AU - Casuso, Rafael A

N1 - CURIS 2019 NEXS 260

PY - 2019

Y1 - 2019

N2 - The main objective of this work was to investigate whether mitochondrial fusion occurs in the skeletal muscle of well-trained athletes in response to high-intensity exercise. Well-trained swimmers (n = 9) performed a duration-matched sprint interval training (SIT) and high-intensity high-volume training (HIHVT) session on separate days. Muscle samples from M. triceps brachii were taken before, immediately after, and 3 h after the training sessions. Transmission electron microscopy (TEM) was applied to assess mitochondrial morphology. Moreover, expression of genes coding for regulators of mitochondrial fusion and fission were assessed by real-time quantitative PCR. In addition, mitofusin (MFN)2 and optic atrophy 1 (OPA1) were quantified by Western blot analysis. TEM analyses showed that mitochondrial morphology remained altered for 3 h after HIHVT, whereas SIT-induced changes were only evident immediately after exercise. Only SIT increased MFN1 and MFN2 mRNA expression, whereas SIT and HIHVT both increased MFN2 protein content 3 h after exercise. Notably, only HIHVT increased OPA1 protein content. Mitochondrial morphologic changes that suggest fusion occurs in well-adapted athletes during exercise. However, HIHVT appears as a more robust inducer of mitochondrial fusion events than SIT. Indeed, SIT induces a rapid and transient change in mitochondrial morphology.

AB - The main objective of this work was to investigate whether mitochondrial fusion occurs in the skeletal muscle of well-trained athletes in response to high-intensity exercise. Well-trained swimmers (n = 9) performed a duration-matched sprint interval training (SIT) and high-intensity high-volume training (HIHVT) session on separate days. Muscle samples from M. triceps brachii were taken before, immediately after, and 3 h after the training sessions. Transmission electron microscopy (TEM) was applied to assess mitochondrial morphology. Moreover, expression of genes coding for regulators of mitochondrial fusion and fission were assessed by real-time quantitative PCR. In addition, mitofusin (MFN)2 and optic atrophy 1 (OPA1) were quantified by Western blot analysis. TEM analyses showed that mitochondrial morphology remained altered for 3 h after HIHVT, whereas SIT-induced changes were only evident immediately after exercise. Only SIT increased MFN1 and MFN2 mRNA expression, whereas SIT and HIHVT both increased MFN2 protein content 3 h after exercise. Notably, only HIHVT increased OPA1 protein content. Mitochondrial morphologic changes that suggest fusion occurs in well-adapted athletes during exercise. However, HIHVT appears as a more robust inducer of mitochondrial fusion events than SIT. Indeed, SIT induces a rapid and transient change in mitochondrial morphology.

KW - Faculty of Science

KW - Physical activity

KW - Mitochondrial fission

KW - Skeletal muscle

KW - Mitofusin 2

U2 - 10.1096/fj.201900365RR

DO - 10.1096/fj.201900365RR

M3 - Journal article

C2 - 31398297

VL - 33

SP - 12087

EP - 12098

JO - F A S E B Journal

JF - F A S E B Journal

SN - 0892-6638

IS - 11

ER -

ID: 225954820