Genes involved in oxidative stress pathways are differentially expressed in circulating mononuclear cells derived from obese insulin-resistant and lean insulin-sensitive individuals following a single mixed-meal challenge

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Genes involved in oxidative stress pathways are differentially expressed in circulating mononuclear cells derived from obese insulin-resistant and lean insulin-sensitive individuals following a single mixed-meal challenge. / Baig, Sonia; Parvaresh Rizi, Ehsan; Chia, Chelsea; Shabeer, Muhammad; Aung, Nweni; Loh, Tze Ping; Magkos, Faidon; Vidal-Puig, Antonio; Seet, Raymond C S; Khoo, Chin Meng; Toh, Sue-Anne.

I: Frontiers in Endocrinology, Bind 10, 256, 2019.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Baig, S, Parvaresh Rizi, E, Chia, C, Shabeer, M, Aung, N, Loh, TP, Magkos, F, Vidal-Puig, A, Seet, RCS, Khoo, CM & Toh, S-A 2019, 'Genes involved in oxidative stress pathways are differentially expressed in circulating mononuclear cells derived from obese insulin-resistant and lean insulin-sensitive individuals following a single mixed-meal challenge', Frontiers in Endocrinology, bind 10, 256. https://doi.org/10.3389/fendo.2019.00256

APA

Baig, S., Parvaresh Rizi, E., Chia, C., Shabeer, M., Aung, N., Loh, T. P., Magkos, F., Vidal-Puig, A., Seet, R. C. S., Khoo, C. M., & Toh, S-A. (2019). Genes involved in oxidative stress pathways are differentially expressed in circulating mononuclear cells derived from obese insulin-resistant and lean insulin-sensitive individuals following a single mixed-meal challenge. Frontiers in Endocrinology, 10, [256]. https://doi.org/10.3389/fendo.2019.00256

Vancouver

Baig S, Parvaresh Rizi E, Chia C, Shabeer M, Aung N, Loh TP o.a. Genes involved in oxidative stress pathways are differentially expressed in circulating mononuclear cells derived from obese insulin-resistant and lean insulin-sensitive individuals following a single mixed-meal challenge. Frontiers in Endocrinology. 2019;10. 256. https://doi.org/10.3389/fendo.2019.00256

Author

Baig, Sonia ; Parvaresh Rizi, Ehsan ; Chia, Chelsea ; Shabeer, Muhammad ; Aung, Nweni ; Loh, Tze Ping ; Magkos, Faidon ; Vidal-Puig, Antonio ; Seet, Raymond C S ; Khoo, Chin Meng ; Toh, Sue-Anne. / Genes involved in oxidative stress pathways are differentially expressed in circulating mononuclear cells derived from obese insulin-resistant and lean insulin-sensitive individuals following a single mixed-meal challenge. I: Frontiers in Endocrinology. 2019 ; Bind 10.

Bibtex

@article{336d5500188640f7a537d90a3f13e254,
title = "Genes involved in oxidative stress pathways are differentially expressed in circulating mononuclear cells derived from obese insulin-resistant and lean insulin-sensitive individuals following a single mixed-meal challenge",
abstract = "Background: Oxidative stress induced by nutritional overload has been linked to the pathogenesis of insulin resistance, which is associated with metabolic syndrome, obesity, type 2 diabetes and diabetic vascular complications. Postprandial changes in expression of oxidative stress pathway genes in obese vs. lean individuals, following intake of different types of meals varying in macronutrient composition have not been characterized to date. Here we aimed to test whether/how oxidative stress responses in obese vs. lean individuals are modulated by meal composition. Methods: High-carbohydrate (HC), high-fat (HF), or high-protein (HP) liquid mixed meals were administered to study subjects (lean insulin-sensitive, n = 9 and obese insulin-resistant, n = 9). Plasma levels of glucose and insulin, lipid profile, urinary F2-isoprostanes (F2-IsoP), and expression levels of genes of oxidative stress pathways were assessed in mononuclear cells (MNC) derived from fresh peripheral blood, at baseline and up to 6-h postprandial states. Differences in these parameters were compared between insulin-sensitive/resistant groups undergoing aforementioned meal challenges. Results: Obese individuals exhibited increased pro-oxidant (i.e., CYBB and CYBA) and anti-oxidant (i.e., TXN RD1) gene expression in the postprandial state, compared with lean subjects, regardless of meal type (P interaction for group × time < 0.05). By contrast, lean subjects had higher expression of NCF-4 gene (pro-oxidant) after HC meal and SOD1 gene (anti-oxidant) after HC and HF meals (P interaction for group × meal < 0.05). There was an increase in postprandial level of urinary F2-IsoP in the obese (P < 0.05) but not lean group. Conclusions: These findings may represent an adaptive oxidative response to mitigate increased stress induced by acute nutritional excess. Further, the results suggest an increased predisposition of obese subjects to oxidative stress. Chronic nutritional excess resulting in increases in body weight and adiposity might lead to decompensation leading to worsening insulin resistance and its sequel. Insights from this study could impact on nutritional recommendations for obese subjects at high-risk of cardiovascular diseases.",
keywords = "Faculty of Science, Obesity, Mononuclear cells, Oxidative stress, Gene expression, Macronutrients",
author = "Sonia Baig and {Parvaresh Rizi}, Ehsan and Chelsea Chia and Muhammad Shabeer and Nweni Aung and Loh, {Tze Ping} and Faidon Magkos and Antonio Vidal-Puig and Seet, {Raymond C S} and Khoo, {Chin Meng} and Sue-Anne Toh",
note = "(Ekstern)",
year = "2019",
doi = "10.3389/fendo.2019.00256",
language = "English",
volume = "10",
journal = "Frontiers in Endocrinology",
issn = "1664-2392",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Genes involved in oxidative stress pathways are differentially expressed in circulating mononuclear cells derived from obese insulin-resistant and lean insulin-sensitive individuals following a single mixed-meal challenge

AU - Baig, Sonia

AU - Parvaresh Rizi, Ehsan

AU - Chia, Chelsea

AU - Shabeer, Muhammad

AU - Aung, Nweni

AU - Loh, Tze Ping

AU - Magkos, Faidon

AU - Vidal-Puig, Antonio

AU - Seet, Raymond C S

AU - Khoo, Chin Meng

AU - Toh, Sue-Anne

N1 - (Ekstern)

PY - 2019

Y1 - 2019

N2 - Background: Oxidative stress induced by nutritional overload has been linked to the pathogenesis of insulin resistance, which is associated with metabolic syndrome, obesity, type 2 diabetes and diabetic vascular complications. Postprandial changes in expression of oxidative stress pathway genes in obese vs. lean individuals, following intake of different types of meals varying in macronutrient composition have not been characterized to date. Here we aimed to test whether/how oxidative stress responses in obese vs. lean individuals are modulated by meal composition. Methods: High-carbohydrate (HC), high-fat (HF), or high-protein (HP) liquid mixed meals were administered to study subjects (lean insulin-sensitive, n = 9 and obese insulin-resistant, n = 9). Plasma levels of glucose and insulin, lipid profile, urinary F2-isoprostanes (F2-IsoP), and expression levels of genes of oxidative stress pathways were assessed in mononuclear cells (MNC) derived from fresh peripheral blood, at baseline and up to 6-h postprandial states. Differences in these parameters were compared between insulin-sensitive/resistant groups undergoing aforementioned meal challenges. Results: Obese individuals exhibited increased pro-oxidant (i.e., CYBB and CYBA) and anti-oxidant (i.e., TXN RD1) gene expression in the postprandial state, compared with lean subjects, regardless of meal type (P interaction for group × time < 0.05). By contrast, lean subjects had higher expression of NCF-4 gene (pro-oxidant) after HC meal and SOD1 gene (anti-oxidant) after HC and HF meals (P interaction for group × meal < 0.05). There was an increase in postprandial level of urinary F2-IsoP in the obese (P < 0.05) but not lean group. Conclusions: These findings may represent an adaptive oxidative response to mitigate increased stress induced by acute nutritional excess. Further, the results suggest an increased predisposition of obese subjects to oxidative stress. Chronic nutritional excess resulting in increases in body weight and adiposity might lead to decompensation leading to worsening insulin resistance and its sequel. Insights from this study could impact on nutritional recommendations for obese subjects at high-risk of cardiovascular diseases.

AB - Background: Oxidative stress induced by nutritional overload has been linked to the pathogenesis of insulin resistance, which is associated with metabolic syndrome, obesity, type 2 diabetes and diabetic vascular complications. Postprandial changes in expression of oxidative stress pathway genes in obese vs. lean individuals, following intake of different types of meals varying in macronutrient composition have not been characterized to date. Here we aimed to test whether/how oxidative stress responses in obese vs. lean individuals are modulated by meal composition. Methods: High-carbohydrate (HC), high-fat (HF), or high-protein (HP) liquid mixed meals were administered to study subjects (lean insulin-sensitive, n = 9 and obese insulin-resistant, n = 9). Plasma levels of glucose and insulin, lipid profile, urinary F2-isoprostanes (F2-IsoP), and expression levels of genes of oxidative stress pathways were assessed in mononuclear cells (MNC) derived from fresh peripheral blood, at baseline and up to 6-h postprandial states. Differences in these parameters were compared between insulin-sensitive/resistant groups undergoing aforementioned meal challenges. Results: Obese individuals exhibited increased pro-oxidant (i.e., CYBB and CYBA) and anti-oxidant (i.e., TXN RD1) gene expression in the postprandial state, compared with lean subjects, regardless of meal type (P interaction for group × time < 0.05). By contrast, lean subjects had higher expression of NCF-4 gene (pro-oxidant) after HC meal and SOD1 gene (anti-oxidant) after HC and HF meals (P interaction for group × meal < 0.05). There was an increase in postprandial level of urinary F2-IsoP in the obese (P < 0.05) but not lean group. Conclusions: These findings may represent an adaptive oxidative response to mitigate increased stress induced by acute nutritional excess. Further, the results suggest an increased predisposition of obese subjects to oxidative stress. Chronic nutritional excess resulting in increases in body weight and adiposity might lead to decompensation leading to worsening insulin resistance and its sequel. Insights from this study could impact on nutritional recommendations for obese subjects at high-risk of cardiovascular diseases.

KW - Faculty of Science

KW - Obesity

KW - Mononuclear cells

KW - Oxidative stress

KW - Gene expression

KW - Macronutrients

U2 - 10.3389/fendo.2019.00256

DO - 10.3389/fendo.2019.00256

M3 - Journal article

C2 - 31068904

VL - 10

JO - Frontiers in Endocrinology

JF - Frontiers in Endocrinology

SN - 1664-2392

M1 - 256

ER -

ID: 217936872