Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing
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- s41467-020-16208-6
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Manipulation of proteins by chemical modification is a powerful way to decipher their function. However, most ribosome-dependent and semi-synthetic methods have limitations in the number and type of modifications that can be introduced, especially in live cells. Here, we present an approach to incorporate single or multiple post-translational modifications or non-canonical amino acids into proteins expressed in eukaryotic cells. We insert synthetic peptides into GFP, NaV1.5 and P2X2 receptors via tandem protein trans-splicing using two orthogonal split intein pairs and validate our approach by investigating protein function. We anticipate the approach will overcome some drawbacks of existing protein enigineering methods.
Originalsprog | Engelsk |
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Artikelnummer | 2284 |
Tidsskrift | Nature Communications |
Vol/bind | 11 |
Udgave nummer | 1 |
Antal sider | 10 |
ISSN | 2041-1723 |
DOI | |
Status | Udgivet - 1 dec. 2020 |
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