TY - JOUR

T1 - Breastmilk lipids and oligosaccharides influence branched short-chain fatty acid concentrations in infants with excessive weight gain

AU - Pekmez, Ceyda Tugba

AU - Larsson, Melanie Wange

AU - Lind, Mads Vendelbo

AU - Manjarrez, Natalia Vazquez

AU - Yonemitsu, Chloe

AU - Larnkjær, Anni

AU - Bode, Lars

AU - Mølgaard, Christian

AU - Michaelsen, Kim F.

AU - Dragsted, Lars Ove

N1 - This article is protected by copyright. All rights reserved.

PY - 2020

Y1 - 2020

N2 - Scope: We aimed to identify breastmilk components associated with fecal concentration of SCFAs and to investigate whether they differ between infants with high weight gain (HW) and normal weight gain (NW).Methods and results: Breastmilk and fecal samples were collected from mother-infant dyads with HW (n = 11) and NW (n = 15) at 5 and 9 months of age. Breastmilk was profiled using an untargeted method on UPLC-qTOF-MS. Fecal SCFAs were quantified using an isotope-labeled chemical derivatization method. Human milk oligosaccharides (HMOs) were quantified using HPLC after fluorescent derivatization. We found lower levels of α-linolenic acid, oleic acid, 3-oxohexadecanoic acid, LPE (P-16:0), LPC (16:0), LPC (18:0), PC (36:2) in breastmilk from mothers from the HW-group at 5 months of age. Fecal SCFA concentrations were increased during the transition period from breastfeeding to complementary feeding. Fecal butyrate concentration was higher in the NW-group at 9 months of age. Fecal branched SCFAs were positively associated with breastmilk phospholipid levels, free-fatty acid levels, HMO-diversity, sialylated-HMOs, 6'-sialyllactose, and disialyl-lacto-N-hexaose.Conclusion: Fecal branched SCFA concentrations seem to be affected by breastmilk lipid and HMO composition. These differences in breastmilk phospholipids, α-linolenic acid, and specific HMO structures may partially explain the excessive weight gain in early life.

AB - Scope: We aimed to identify breastmilk components associated with fecal concentration of SCFAs and to investigate whether they differ between infants with high weight gain (HW) and normal weight gain (NW).Methods and results: Breastmilk and fecal samples were collected from mother-infant dyads with HW (n = 11) and NW (n = 15) at 5 and 9 months of age. Breastmilk was profiled using an untargeted method on UPLC-qTOF-MS. Fecal SCFAs were quantified using an isotope-labeled chemical derivatization method. Human milk oligosaccharides (HMOs) were quantified using HPLC after fluorescent derivatization. We found lower levels of α-linolenic acid, oleic acid, 3-oxohexadecanoic acid, LPE (P-16:0), LPC (16:0), LPC (18:0), PC (36:2) in breastmilk from mothers from the HW-group at 5 months of age. Fecal SCFA concentrations were increased during the transition period from breastfeeding to complementary feeding. Fecal butyrate concentration was higher in the NW-group at 9 months of age. Fecal branched SCFAs were positively associated with breastmilk phospholipid levels, free-fatty acid levels, HMO-diversity, sialylated-HMOs, 6'-sialyllactose, and disialyl-lacto-N-hexaose.Conclusion: Fecal branched SCFA concentrations seem to be affected by breastmilk lipid and HMO composition. These differences in breastmilk phospholipids, α-linolenic acid, and specific HMO structures may partially explain the excessive weight gain in early life.

KW - Faculty of Science

KW - Isobutyrate

KW - Isovalerate

KW - 2-methylbutyrate

KW - Gut fermentation

KW - Proteolytic bacteria

U2 - 10.1002/mnfr.201900977

DO - 10.1002/mnfr.201900977

M3 - Journal article

C2 - 31801176

VL - 64

JO - Molecular Nutrition & Food Research

JF - Molecular Nutrition & Food Research

SN - 1613-4125

IS - 3

M1 - 1900977

ER -