α-MSH stimulates glucose uptake in mouse muscle and phosphorylates Rab-GTPase-activating protein TBC1D1 independently of AMPK

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

α-MSH stimulates glucose uptake in mouse muscle and phosphorylates Rab-GTPase-activating protein TBC1D1 independently of AMPK. / Møller, Cathrine Laustrup; Kjøbsted, Rasmus; Enriori, Pablo J; Jensen, Thomas Elbenhardt; Garcia-Rudaz, Cecilia; Litwak, Sara A; Raun, Kirsten; Wojtaszewski, Jørgen; Wulff, Birgitte Schjellerup; Cowley, Michael A.

I: P L o S One, Bind 11, Nr. 7, e0157027, 2016.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Møller, CL, Kjøbsted, R, Enriori, PJ, Jensen, TE, Garcia-Rudaz, C, Litwak, SA, Raun, K, Wojtaszewski, J, Wulff, BS & Cowley, MA 2016, 'α-MSH stimulates glucose uptake in mouse muscle and phosphorylates Rab-GTPase-activating protein TBC1D1 independently of AMPK', P L o S One, bind 11, nr. 7, e0157027. https://doi.org/10.1371/journal.pone.0157027

APA

Møller, C. L., Kjøbsted, R., Enriori, P. J., Jensen, T. E., Garcia-Rudaz, C., Litwak, S. A., ... Cowley, M. A. (2016). α-MSH stimulates glucose uptake in mouse muscle and phosphorylates Rab-GTPase-activating protein TBC1D1 independently of AMPK. P L o S One, 11(7), [e0157027]. https://doi.org/10.1371/journal.pone.0157027

Vancouver

Møller CL, Kjøbsted R, Enriori PJ, Jensen TE, Garcia-Rudaz C, Litwak SA o.a. α-MSH stimulates glucose uptake in mouse muscle and phosphorylates Rab-GTPase-activating protein TBC1D1 independently of AMPK. P L o S One. 2016;11(7). e0157027. https://doi.org/10.1371/journal.pone.0157027

Author

Møller, Cathrine Laustrup ; Kjøbsted, Rasmus ; Enriori, Pablo J ; Jensen, Thomas Elbenhardt ; Garcia-Rudaz, Cecilia ; Litwak, Sara A ; Raun, Kirsten ; Wojtaszewski, Jørgen ; Wulff, Birgitte Schjellerup ; Cowley, Michael A. / α-MSH stimulates glucose uptake in mouse muscle and phosphorylates Rab-GTPase-activating protein TBC1D1 independently of AMPK. I: P L o S One. 2016 ; Bind 11, Nr. 7.

Bibtex

@article{ad9b5b25255e408b89971c7c108b1744,
title = "α-MSH stimulates glucose uptake in mouse muscle and phosphorylates Rab-GTPase-activating protein TBC1D1 independently of AMPK",
abstract = "The melanocortin system includes five G-protein coupled receptors (family A) defined as MC1R-MC5R, which are stimulated by endogenous agonists derived from proopiomelanocortin (POMC). The melanocortin system has been intensely studied for its central actions in body weight and energy expenditure regulation, which are mainly mediated by MC4R. The pituitary gland is the source of various POMC-derived hormones released to the circulation, which raises the possibility that there may be actions of the melanocortins on peripheral energy homeostasis. In this study, we examined the molecular signaling pathway involved in α-MSH-stimulated glucose uptake in differentiated L6 myotubes and mouse muscle explants. In order to examine the involvement of AMPK, we investigate -MSH stimulation in both wild type and AMPK deficient mice. We found that -MSH significantly induces phosphorylation of TBC1 domain (TBC1D) family member 1 (S237 and T596), which is independent of upstream PKA and AMPK. We find no evidence to support that -MSH-stimulated glucose uptake involves TBC1D4 phosphorylation (T642 and S704) or GLUT4 translocation.",
keywords = "Journal Article",
author = "M{\o}ller, {Cathrine Laustrup} and Rasmus Kj{\o}bsted and Enriori, {Pablo J} and Jensen, {Thomas Elbenhardt} and Cecilia Garcia-Rudaz and Litwak, {Sara A} and Kirsten Raun and J{\o}rgen Wojtaszewski and Wulff, {Birgitte Schjellerup} and Cowley, {Michael A}",
note = "CURIS 2016 NEXS 208",
year = "2016",
doi = "10.1371/journal.pone.0157027",
language = "English",
volume = "11",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "7",

}

RIS

TY - JOUR

T1 - α-MSH stimulates glucose uptake in mouse muscle and phosphorylates Rab-GTPase-activating protein TBC1D1 independently of AMPK

AU - Møller, Cathrine Laustrup

AU - Kjøbsted, Rasmus

AU - Enriori, Pablo J

AU - Jensen, Thomas Elbenhardt

AU - Garcia-Rudaz, Cecilia

AU - Litwak, Sara A

AU - Raun, Kirsten

AU - Wojtaszewski, Jørgen

AU - Wulff, Birgitte Schjellerup

AU - Cowley, Michael A

N1 - CURIS 2016 NEXS 208

PY - 2016

Y1 - 2016

N2 - The melanocortin system includes five G-protein coupled receptors (family A) defined as MC1R-MC5R, which are stimulated by endogenous agonists derived from proopiomelanocortin (POMC). The melanocortin system has been intensely studied for its central actions in body weight and energy expenditure regulation, which are mainly mediated by MC4R. The pituitary gland is the source of various POMC-derived hormones released to the circulation, which raises the possibility that there may be actions of the melanocortins on peripheral energy homeostasis. In this study, we examined the molecular signaling pathway involved in α-MSH-stimulated glucose uptake in differentiated L6 myotubes and mouse muscle explants. In order to examine the involvement of AMPK, we investigate -MSH stimulation in both wild type and AMPK deficient mice. We found that -MSH significantly induces phosphorylation of TBC1 domain (TBC1D) family member 1 (S237 and T596), which is independent of upstream PKA and AMPK. We find no evidence to support that -MSH-stimulated glucose uptake involves TBC1D4 phosphorylation (T642 and S704) or GLUT4 translocation.

AB - The melanocortin system includes five G-protein coupled receptors (family A) defined as MC1R-MC5R, which are stimulated by endogenous agonists derived from proopiomelanocortin (POMC). The melanocortin system has been intensely studied for its central actions in body weight and energy expenditure regulation, which are mainly mediated by MC4R. The pituitary gland is the source of various POMC-derived hormones released to the circulation, which raises the possibility that there may be actions of the melanocortins on peripheral energy homeostasis. In this study, we examined the molecular signaling pathway involved in α-MSH-stimulated glucose uptake in differentiated L6 myotubes and mouse muscle explants. In order to examine the involvement of AMPK, we investigate -MSH stimulation in both wild type and AMPK deficient mice. We found that -MSH significantly induces phosphorylation of TBC1 domain (TBC1D) family member 1 (S237 and T596), which is independent of upstream PKA and AMPK. We find no evidence to support that -MSH-stimulated glucose uptake involves TBC1D4 phosphorylation (T642 and S704) or GLUT4 translocation.

KW - Journal Article

U2 - 10.1371/journal.pone.0157027

DO - 10.1371/journal.pone.0157027

M3 - Journal article

C2 - 27467141

VL - 11

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 7

M1 - e0157027

ER -

ID: 164456107