FADS1 genotype is distinguished by human subcutaneous adipose tissue fatty acids, but not inflammatory gene expression

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FADS1 genotype is distinguished by human subcutaneous adipose tissue fatty acids, but not inflammatory gene expression. / Klingel, Shannon L; Valsesia, Armand; Astrup, Arne; Kunesova, Marie; Saris, Wim H M; Langin, Dominique; Viguerie, Nathalie; Mutch, David M.

I: International Journal of Obesity, Bind 43, 2019, s. 1539-1548.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Klingel, SL, Valsesia, A, Astrup, A, Kunesova, M, Saris, WHM, Langin, D, Viguerie, N & Mutch, DM 2019, 'FADS1 genotype is distinguished by human subcutaneous adipose tissue fatty acids, but not inflammatory gene expression', International Journal of Obesity, bind 43, s. 1539-1548. https://doi.org/10.1038/s41366-018-0169-z

APA

Klingel, S. L., Valsesia, A., Astrup, A., Kunesova, M., Saris, W. H. M., Langin, D., ... Mutch, D. M. (2019). FADS1 genotype is distinguished by human subcutaneous adipose tissue fatty acids, but not inflammatory gene expression. International Journal of Obesity, 43, 1539-1548. https://doi.org/10.1038/s41366-018-0169-z

Vancouver

Klingel SL, Valsesia A, Astrup A, Kunesova M, Saris WHM, Langin D o.a. FADS1 genotype is distinguished by human subcutaneous adipose tissue fatty acids, but not inflammatory gene expression. International Journal of Obesity. 2019;43:1539-1548. https://doi.org/10.1038/s41366-018-0169-z

Author

Klingel, Shannon L ; Valsesia, Armand ; Astrup, Arne ; Kunesova, Marie ; Saris, Wim H M ; Langin, Dominique ; Viguerie, Nathalie ; Mutch, David M. / FADS1 genotype is distinguished by human subcutaneous adipose tissue fatty acids, but not inflammatory gene expression. I: International Journal of Obesity. 2019 ; Bind 43. s. 1539-1548.

Bibtex

@article{38d0d6f6ef8d45e0bad8b50ab6c7eaef,
title = "FADS1 genotype is distinguished by human subcutaneous adipose tissue fatty acids, but not inflammatory gene expression",
abstract = "Background: Single nucleotide polymorphisms (SNPs) in FADS1/FADS2 genes are associated with changes in serum and tissue polyunsaturated fatty acid (PUFA) content. PUFA regulate inflammatory signaling pathways in adipose tissue; however, the effect of SNPs in FADS1/FADS2 on adipose tissue inflammation is equivocal. The present study examined if SNPs in FADS1/FADS2 modify human subcutaneous adipose tissue (SAT) fatty acid profiles and the expression of genes associated with inflammation/immune function, lipid metabolism, and cellular differentiation.Methods: SAT fatty acids and the expression of 117 genes were measured in 174 men and women from the DiOGenes Study using gas chromatography and qRT-PCR, respectively. Associations between fatty acids, gene expression, and SNPs in FADS1/FADS2 were investigated by linear regression and multivariate analysis.Results: Four SNPs (rs174537, rs174546, rs174556, rs174601) in FADS1/FADS2 were significantly associated with SAT fatty acids. All SNPs were in high linkage disequilibrium with the commonly reported rs174537 SNP in FADS1. Minor allele carriers for rs174537 (GT+TT) had reduced 20:4n-6 (p = 1.74E-5), lower delta-5 desaturase enzyme activity (p = 2.09E-9), and lower FADS1 gene expression (p = 0.03) compared to major GG carriers. Multivariate analysis revealed that 20:4n-6 and 20:3n-6 explained ~19{\%} of the variance between rs174537 genotypes, while gene expression explained <7{\%}. Receiver operating characteristic (ROC) curves indicated that rs174537 genotype can be distinguished with SAT fatty acids (AUC = 0.842), but not gene expression (AUC = 0.627). No differences in SAT inflammatory gene expression were observed between rs174537 genotypes. SAT 20:3n-6 levels were positively correlated with the expression of several inflammatory genes, and inversely correlated with FADS1 expression.Conclusion: This study showed that FADS1 genotype is distinguished by SAT fatty acid profiles, but not inflammatory gene expression.",
author = "Klingel, {Shannon L} and Armand Valsesia and Arne Astrup and Marie Kunesova and Saris, {Wim H M} and Dominique Langin and Nathalie Viguerie and Mutch, {David M}",
note = "CURIS 2019 NEXS 266",
year = "2019",
doi = "10.1038/s41366-018-0169-z",
language = "English",
volume = "43",
pages = "1539--1548",
journal = "International Journal of Obesity",
issn = "0307-0565",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - FADS1 genotype is distinguished by human subcutaneous adipose tissue fatty acids, but not inflammatory gene expression

AU - Klingel, Shannon L

AU - Valsesia, Armand

AU - Astrup, Arne

AU - Kunesova, Marie

AU - Saris, Wim H M

AU - Langin, Dominique

AU - Viguerie, Nathalie

AU - Mutch, David M

N1 - CURIS 2019 NEXS 266

PY - 2019

Y1 - 2019

N2 - Background: Single nucleotide polymorphisms (SNPs) in FADS1/FADS2 genes are associated with changes in serum and tissue polyunsaturated fatty acid (PUFA) content. PUFA regulate inflammatory signaling pathways in adipose tissue; however, the effect of SNPs in FADS1/FADS2 on adipose tissue inflammation is equivocal. The present study examined if SNPs in FADS1/FADS2 modify human subcutaneous adipose tissue (SAT) fatty acid profiles and the expression of genes associated with inflammation/immune function, lipid metabolism, and cellular differentiation.Methods: SAT fatty acids and the expression of 117 genes were measured in 174 men and women from the DiOGenes Study using gas chromatography and qRT-PCR, respectively. Associations between fatty acids, gene expression, and SNPs in FADS1/FADS2 were investigated by linear regression and multivariate analysis.Results: Four SNPs (rs174537, rs174546, rs174556, rs174601) in FADS1/FADS2 were significantly associated with SAT fatty acids. All SNPs were in high linkage disequilibrium with the commonly reported rs174537 SNP in FADS1. Minor allele carriers for rs174537 (GT+TT) had reduced 20:4n-6 (p = 1.74E-5), lower delta-5 desaturase enzyme activity (p = 2.09E-9), and lower FADS1 gene expression (p = 0.03) compared to major GG carriers. Multivariate analysis revealed that 20:4n-6 and 20:3n-6 explained ~19% of the variance between rs174537 genotypes, while gene expression explained <7%. Receiver operating characteristic (ROC) curves indicated that rs174537 genotype can be distinguished with SAT fatty acids (AUC = 0.842), but not gene expression (AUC = 0.627). No differences in SAT inflammatory gene expression were observed between rs174537 genotypes. SAT 20:3n-6 levels were positively correlated with the expression of several inflammatory genes, and inversely correlated with FADS1 expression.Conclusion: This study showed that FADS1 genotype is distinguished by SAT fatty acid profiles, but not inflammatory gene expression.

AB - Background: Single nucleotide polymorphisms (SNPs) in FADS1/FADS2 genes are associated with changes in serum and tissue polyunsaturated fatty acid (PUFA) content. PUFA regulate inflammatory signaling pathways in adipose tissue; however, the effect of SNPs in FADS1/FADS2 on adipose tissue inflammation is equivocal. The present study examined if SNPs in FADS1/FADS2 modify human subcutaneous adipose tissue (SAT) fatty acid profiles and the expression of genes associated with inflammation/immune function, lipid metabolism, and cellular differentiation.Methods: SAT fatty acids and the expression of 117 genes were measured in 174 men and women from the DiOGenes Study using gas chromatography and qRT-PCR, respectively. Associations between fatty acids, gene expression, and SNPs in FADS1/FADS2 were investigated by linear regression and multivariate analysis.Results: Four SNPs (rs174537, rs174546, rs174556, rs174601) in FADS1/FADS2 were significantly associated with SAT fatty acids. All SNPs were in high linkage disequilibrium with the commonly reported rs174537 SNP in FADS1. Minor allele carriers for rs174537 (GT+TT) had reduced 20:4n-6 (p = 1.74E-5), lower delta-5 desaturase enzyme activity (p = 2.09E-9), and lower FADS1 gene expression (p = 0.03) compared to major GG carriers. Multivariate analysis revealed that 20:4n-6 and 20:3n-6 explained ~19% of the variance between rs174537 genotypes, while gene expression explained <7%. Receiver operating characteristic (ROC) curves indicated that rs174537 genotype can be distinguished with SAT fatty acids (AUC = 0.842), but not gene expression (AUC = 0.627). No differences in SAT inflammatory gene expression were observed between rs174537 genotypes. SAT 20:3n-6 levels were positively correlated with the expression of several inflammatory genes, and inversely correlated with FADS1 expression.Conclusion: This study showed that FADS1 genotype is distinguished by SAT fatty acid profiles, but not inflammatory gene expression.

U2 - 10.1038/s41366-018-0169-z

DO - 10.1038/s41366-018-0169-z

M3 - Journal article

C2 - 30082751

VL - 43

SP - 1539

EP - 1548

JO - International Journal of Obesity

JF - International Journal of Obesity

SN - 0307-0565

ER -

ID: 201041176