Comprehensive profiling of the circulatory miRNAome response to a high protein diet in elderly men: A potential role in inflammatory response modulation

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Farha Ramzan, Cameron J Mitchell, Amber M Milan, William Schierding, Nina Zeng, Pankaja Sharma, Sarah M Mitchell, Randall F D'Souza, Scott O Knowles, Nicole C Roy, Anders Mikael Sjödin, Karl-Heinz Wagner, David Cameron-Smith

Scope: MicroRNA are critical to the coordinated post-transcriptional regulation of gene expression, yet few studies have addressed the influence of habitual diet on microRNA expression. High protein diets impact cardiometabolic health and body composition in the elderly suggesting the possibility of a complex systems response. Therefore, high-throughput small RNA sequencing technology is applied in response to doubling the protein recommended dietary allowance (RDA) over 10 weeks in older men to examine alterations in circulating miRNAome.

Methods and Results: Older men (n = 31; 74.1 ± 0.6 y) are randomized to consume either RDA (0.8 g kg −1  day −1 ) or 2RDA (1.6 g kg −1  day −1 ) of protein for 10 weeks. Downregulation of five microRNAs (miR-125b-5p, -100-5p, -99a-5p, -23b-3p, and -203a) is observed following 2RDA with no changes in the RDA. In silico functional analysis highlights target gene enrichment in inflammation-related pathways. qPCR quantification of predicted inflammatory genes (TNFα, IL-8, IL-6, pTEN, PPP1CB, and HOXA1) in peripheral blood mononuclear cells shows increased expression following 2RDA diet (p ≤ 0.05).

Conclusion: The study findings suggest a possible selective alteration in the post-transcriptional regulation of the immune system following a high protein diet. However, very few microRNAs are altered despite a large change in the dietary protein.

OriginalsprogEngelsk
Artikelnummer1800811
TidsskriftMolecular Nutrition & Food Research
Vol/bind63
Udgave nummer8
Antal sider10
ISSN1613-4125
DOI
StatusUdgivet - 2019

Bibliografisk note

CURIS 2019 NEXS 144

ID: 217112603