Column-switching high-performance liquid chromatographic assay for determination of apigenin and acacetin in human urine with ultraviolet absorbance detection

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A high-performance liquid chromatographic (HPLC) method is described for the determination of apigenin and the 4'-methylated derivative acacetin in human urine using column-switching and ultraviolet (UV) absorbance detection. Urine samples were enzymatically hydrolysed and solid-phase extracted prior to injection onto the HPLC system. Prior to elution of apigenin and the internal standard, 5,7,8-trihydroxyflavone, from the first column used for sample clean-up, the six-port valve was switched to the second column for analysis with UV detection. Detection of apigenin was precise and reproducible, with a limit of quantification of 10 ngml-1 urine. Detection and quantification of acacetin was linear down to 70 ngml-1 urine. The method has been successfully applied to determine the level of apigenin in 100 human urine samples from an intervention study with parsley. Copyright (C) 1998 Elsevier Science B.V.

TidsskriftJournal of Chromatography B: Biomedical Sciences and Applications
Udgave nummer2
Sider (fra-til)379-386
Antal sider8
StatusUdgivet - 1998

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