Inhibition of muscle glycogen synthase activity and non-oxidative glucose disposal during hypoglycaemia in normal man

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Inhibition of muscle glycogen synthase activity and non-oxidative glucose disposal during hypoglycaemia in normal man. / Ørskov, Lotte; Bak, Jens Friis; Abildgaard, Ulrik; Schmitz, Ole; Andreasen, F; Richter, Erik A.; Skjærbæk, C; Møller, N.

In: Diabetologia, Vol. 39, No. 2, 1996, p. 226-234.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ørskov, L, Bak, JF, Abildgaard, U, Schmitz, O, Andreasen, F, Richter, EA, Skjærbæk, C & Møller, N 1996, 'Inhibition of muscle glycogen synthase activity and non-oxidative glucose disposal during hypoglycaemia in normal man', Diabetologia, vol. 39, no. 2, pp. 226-234.

APA

Ørskov, L., Bak, J. F., Abildgaard, U., Schmitz, O., Andreasen, F., Richter, E. A., Skjærbæk, C., & Møller, N. (1996). Inhibition of muscle glycogen synthase activity and non-oxidative glucose disposal during hypoglycaemia in normal man. Diabetologia, 39(2), 226-234.

Vancouver

Ørskov L, Bak JF, Abildgaard U, Schmitz O, Andreasen F, Richter EA et al. Inhibition of muscle glycogen synthase activity and non-oxidative glucose disposal during hypoglycaemia in normal man. Diabetologia. 1996;39(2):226-234.

Author

Ørskov, Lotte ; Bak, Jens Friis ; Abildgaard, Ulrik ; Schmitz, Ole ; Andreasen, F ; Richter, Erik A. ; Skjærbæk, C ; Møller, N. / Inhibition of muscle glycogen synthase activity and non-oxidative glucose disposal during hypoglycaemia in normal man. In: Diabetologia. 1996 ; Vol. 39, No. 2. pp. 226-234.

Bibtex

@article{bebd9389b36d471aa30d01cbe1785152,
title = "Inhibition of muscle glycogen synthase activity and non-oxidative glucose disposal during hypoglycaemia in normal man",
abstract = "The purpose of the present study was to evaluate the role of muscle glycogen synthase activity in the reduction of glucose uptake during hypoglycaemia. Six healthy young men were examined twice; during 120 min of hyperinsulinaemic (1.5 mU.kg-1. min-1) euglycaemia followed by: 1)240 min of graded hypoglycaemia (plasma glucose nadir 2.8 mmol/l) or 2) 240 min of euglycaemia. At 350-360 min a muscle biopsy was taken and indirect calorimetry was performed at 210-240 and 330-350 min. Hypoglycaemia was associated with markedly increased levels of adrenaline, growth hormone and glucagon and also with less hyperinsulinaemia. During hypoglycaemia the fractional velocity for glycogen synthase was markedly reduced; from 29.8 +/- 2.3 to 6.4 +/- 0.9%, p < 0.05. Total glucose disposal was decreased during hypoglycaemia (5.58 +/- 0.55 vs 11.01 +/- 0.75 mg.kg-1. min-1 (euglycaemia); p < 0.05); this was primarily due to a reduction of non-oxidative glucose disposal (2.43 +/- 0.41 vs 7.15 +/- 0.7 mg.kg-1 .min-1 (euglycaemia); p < 0.05), whereas oxidative glucose disposal was only suppressed to a minor degree. In conclusion hypoglycaemia virtually abolishes the effect of insulin on muscle glycogen synthase activity. This is in keeping with the finding of a marked reduction of non-oxidative glucose metabolism.",
keywords = "Adult, Biopsy, Blood Glucose, C-Peptide, Calorimetry, Indirect, Epinephrine, Fatty Acids, Nonesterified, Glucagon, Glucose Clamp Technique, Glycogen Synthase, Glycolysis, Growth Hormone, Humans, Hypoglycemia, Infusions, Intravenous, Insulin, Kinetics, Male, Muscle, Skeletal, Reference Values, Time Factors",
author = "Lotte {\O}rskov and Bak, {Jens Friis} and Ulrik Abildgaard and Ole Schmitz and F Andreasen and Richter, {Erik A.} and C Skj{\ae}rb{\ae}k and N M{\o}ller",
year = "1996",
language = "English",
volume = "39",
pages = "226--234",
journal = "Diabetologia",
issn = "0012-186X",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Inhibition of muscle glycogen synthase activity and non-oxidative glucose disposal during hypoglycaemia in normal man

AU - Ørskov, Lotte

AU - Bak, Jens Friis

AU - Abildgaard, Ulrik

AU - Schmitz, Ole

AU - Andreasen, F

AU - Richter, Erik A.

AU - Skjærbæk, C

AU - Møller, N

PY - 1996

Y1 - 1996

N2 - The purpose of the present study was to evaluate the role of muscle glycogen synthase activity in the reduction of glucose uptake during hypoglycaemia. Six healthy young men were examined twice; during 120 min of hyperinsulinaemic (1.5 mU.kg-1. min-1) euglycaemia followed by: 1)240 min of graded hypoglycaemia (plasma glucose nadir 2.8 mmol/l) or 2) 240 min of euglycaemia. At 350-360 min a muscle biopsy was taken and indirect calorimetry was performed at 210-240 and 330-350 min. Hypoglycaemia was associated with markedly increased levels of adrenaline, growth hormone and glucagon and also with less hyperinsulinaemia. During hypoglycaemia the fractional velocity for glycogen synthase was markedly reduced; from 29.8 +/- 2.3 to 6.4 +/- 0.9%, p < 0.05. Total glucose disposal was decreased during hypoglycaemia (5.58 +/- 0.55 vs 11.01 +/- 0.75 mg.kg-1. min-1 (euglycaemia); p < 0.05); this was primarily due to a reduction of non-oxidative glucose disposal (2.43 +/- 0.41 vs 7.15 +/- 0.7 mg.kg-1 .min-1 (euglycaemia); p < 0.05), whereas oxidative glucose disposal was only suppressed to a minor degree. In conclusion hypoglycaemia virtually abolishes the effect of insulin on muscle glycogen synthase activity. This is in keeping with the finding of a marked reduction of non-oxidative glucose metabolism.

AB - The purpose of the present study was to evaluate the role of muscle glycogen synthase activity in the reduction of glucose uptake during hypoglycaemia. Six healthy young men were examined twice; during 120 min of hyperinsulinaemic (1.5 mU.kg-1. min-1) euglycaemia followed by: 1)240 min of graded hypoglycaemia (plasma glucose nadir 2.8 mmol/l) or 2) 240 min of euglycaemia. At 350-360 min a muscle biopsy was taken and indirect calorimetry was performed at 210-240 and 330-350 min. Hypoglycaemia was associated with markedly increased levels of adrenaline, growth hormone and glucagon and also with less hyperinsulinaemia. During hypoglycaemia the fractional velocity for glycogen synthase was markedly reduced; from 29.8 +/- 2.3 to 6.4 +/- 0.9%, p < 0.05. Total glucose disposal was decreased during hypoglycaemia (5.58 +/- 0.55 vs 11.01 +/- 0.75 mg.kg-1. min-1 (euglycaemia); p < 0.05); this was primarily due to a reduction of non-oxidative glucose disposal (2.43 +/- 0.41 vs 7.15 +/- 0.7 mg.kg-1 .min-1 (euglycaemia); p < 0.05), whereas oxidative glucose disposal was only suppressed to a minor degree. In conclusion hypoglycaemia virtually abolishes the effect of insulin on muscle glycogen synthase activity. This is in keeping with the finding of a marked reduction of non-oxidative glucose metabolism.

KW - Adult

KW - Biopsy

KW - Blood Glucose

KW - C-Peptide

KW - Calorimetry, Indirect

KW - Epinephrine

KW - Fatty Acids, Nonesterified

KW - Glucagon

KW - Glucose Clamp Technique

KW - Glycogen Synthase

KW - Glycolysis

KW - Growth Hormone

KW - Humans

KW - Hypoglycemia

KW - Infusions, Intravenous

KW - Insulin

KW - Kinetics

KW - Male

KW - Muscle, Skeletal

KW - Reference Values

KW - Time Factors

M3 - Journal article

C2 - 8635676

VL - 39

SP - 226

EP - 234

JO - Diabetologia

JF - Diabetologia

SN - 0012-186X

IS - 2

ER -

ID: 154750300