Role of endocannabinoids in energy-balance regulation in participants in the postobese state - a PREVIEW study

Research output: Contribution to journalJournal articleResearchpeer-review

  • Mathijs Drummen
  • Lea Tischmann
  • Blandine Gatta-Cherifi
  • Daniela Cota
  • Isabelle Matias
  • Raben, Anne
  • Tanja Adam
  • Margriet Westerterp-Plantenga

Context: Endocannabinoids are suggested to play a role in energy balance regulation.

Objective: We aimed to investigate associations of endocannabinoid concentrations during the day with energy balance and adiposity and interactions with 2 diets differing in protein content in participants in the post-obese phase with pre-diabetes.

Design and participants: Participants (n=38) were individually fed in energy balance with a medium protein (MP: 15:55:30% of energy from Protein:Carbohydrate:Fat) or high protein diet (HP: 25:45:30% energy from P:C:F) for 48-hours in a respiration chamber.

Main outcome measures: Associations between energy balance, energy expenditure, RQ and endocannabinoid concentrations during the day were assessed.

Results: Plasma-concentrations of anandamide (AEA), oleoylethanolamide (OEA), palmitoyethanolamide (PEA), and pregnenolone (PREG) significantly decreased during the day. This decrease was inversely related to BMI (AEA) or body-fat (%) (PEA; OEA). The lowest RQ value, before lunch, was inversely associated with concentrations of AEA and PEA before lunch. AUC of concentrations of AEA, 2-AG, PEA, and OEA were positively related to body-fat% (P < 0.05). 

The HP and MP groups showed no differences in concentrations of AEA, OEA, PEA, and PREG, but the AUC of 2-arachidonoylglycerol (2-AG) was significantly higher in the HP vs. the MP group.

Conclusions: In energy balance, only the endocannabinoid 2-AG changed in relation to protein level of the diet, while the endocannabinoid AEA, and endocannabinoid-related compounds OEA and PEA reflected the gradual energy intake matching energy expenditure over the day.

Original languageEnglish
Article numberdgaa193
JournalJournal of Clinical Endocrinology and Metabolism
Issue number7
Number of pages10
Publication statusPublished - 2020

Bibliographical note

© Endocrine Society 2020.

ID: 240979041