Quantification of diacylglycerol and triacylglycerol species in human fecal samples by flow injection Fourier transform mass spectrometry

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Quantification of diacylglycerol and triacylglycerol species in human fecal samples by flow injection Fourier transform mass spectrometry. / Ertl, Verena M; Höring, Marcus; Schött, Hans-Frieder; Blücher, Christina; Kjølbæk, Louise; Astrup, Arne; Burkhardt, Ralph; Liebisch, Gerhard.

In: Analytical and Bioanalytical Chemistry, Vol. 412, No. 10, 2020, p. 2315-2326.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Ertl, VM, Höring, M, Schött, H-F, Blücher, C, Kjølbæk, L, Astrup, A, Burkhardt, R & Liebisch, G 2020, 'Quantification of diacylglycerol and triacylglycerol species in human fecal samples by flow injection Fourier transform mass spectrometry', Analytical and Bioanalytical Chemistry, vol. 412, no. 10, pp. 2315-2326. https://doi.org/10.1007/s00216-020-02416-y

APA

Ertl, V. M., Höring, M., Schött, H-F., Blücher, C., Kjølbæk, L., Astrup, A., ... Liebisch, G. (2020). Quantification of diacylglycerol and triacylglycerol species in human fecal samples by flow injection Fourier transform mass spectrometry. Analytical and Bioanalytical Chemistry, 412(10), 2315-2326. https://doi.org/10.1007/s00216-020-02416-y

Vancouver

Ertl VM, Höring M, Schött H-F, Blücher C, Kjølbæk L, Astrup A et al. Quantification of diacylglycerol and triacylglycerol species in human fecal samples by flow injection Fourier transform mass spectrometry. Analytical and Bioanalytical Chemistry. 2020;412(10):2315-2326. https://doi.org/10.1007/s00216-020-02416-y

Author

Ertl, Verena M ; Höring, Marcus ; Schött, Hans-Frieder ; Blücher, Christina ; Kjølbæk, Louise ; Astrup, Arne ; Burkhardt, Ralph ; Liebisch, Gerhard. / Quantification of diacylglycerol and triacylglycerol species in human fecal samples by flow injection Fourier transform mass spectrometry. In: Analytical and Bioanalytical Chemistry. 2020 ; Vol. 412, No. 10. pp. 2315-2326.

Bibtex

@article{250256bea4b9403aa9cd0e3c810b8d52,
title = "Quantification of diacylglycerol and triacylglycerol species in human fecal samples by flow injection Fourier transform mass spectrometry",
abstract = "The intestinal microbiome plays an important role in human health and disease and fecal materials reflect the microbial activity. Thus, analysis of fecal metabolites provides insight in metabolic interactions between gut microbiota and host organism. In this work, we applied flow injection analysis coupled to Fourier transform mass spectrometry (FIA-FTMS) to identify and quantify lipid species in human fecal samples. Fecal homogenates were subjected to lipid extraction and analyzed by FIA-FTMS. The analysis of different subjects revealed a vast heterogeneity of lipid species abundance. The majority of samples displayed prominent signals of triacylglycerol (TG) and diacylglycerol (DG) species that could be verified by MS2 spectra. Therefore, we focused on the quantification of TG and DG. Method validation included limit of quantification, linearity, evaluation of matrix effects, recovery, and reproducibility. The validation experiments demonstrated the suitability of the method, with exception for approximately 10{\%} of samples, where we observed coefficients of variation higher than 15{\%}. Impaired reproducibility was related to sample inhomogeneity and could not be improved by additional sample preparation steps. Additionally, these experiments demonstrated that compared with aqueous samples, samples containing isopropanol showed higher amounts of DG, presumably due to lysis of bacteria and increased TG lipolysis. These effects were sample-specific and substantiate the high heterogeneity of fecal materials as well as the need for further evaluation of pre-analytic conditions. In summary, FIA-FTMS offers a fast and accurate tool to quantify DG and TG species and is suitable to provide insight into the fecal lipidome and its role in health and disease.",
keywords = "Faculty of Science, Lipidomics, Microbiome, Feces, Triglyceride, Diglyceride, High-resolution mass spectrometry",
author = "Ertl, {Verena M} and Marcus H{\"o}ring and Hans-Frieder Sch{\"o}tt and Christina Bl{\"u}cher and Louise Kj{\o}lb{\ae}k and Arne Astrup and Ralph Burkhardt and Gerhard Liebisch",
note = "CURIS 2020 NEXS 093",
year = "2020",
doi = "10.1007/s00216-020-02416-y",
language = "English",
volume = "412",
pages = "2315--2326",
journal = "Analusis - European Journal of Analytical Chemistry",
issn = "0365-4877",
publisher = "EDP Sciences",
number = "10",

}

RIS

TY - JOUR

T1 - Quantification of diacylglycerol and triacylglycerol species in human fecal samples by flow injection Fourier transform mass spectrometry

AU - Ertl, Verena M

AU - Höring, Marcus

AU - Schött, Hans-Frieder

AU - Blücher, Christina

AU - Kjølbæk, Louise

AU - Astrup, Arne

AU - Burkhardt, Ralph

AU - Liebisch, Gerhard

N1 - CURIS 2020 NEXS 093

PY - 2020

Y1 - 2020

N2 - The intestinal microbiome plays an important role in human health and disease and fecal materials reflect the microbial activity. Thus, analysis of fecal metabolites provides insight in metabolic interactions between gut microbiota and host organism. In this work, we applied flow injection analysis coupled to Fourier transform mass spectrometry (FIA-FTMS) to identify and quantify lipid species in human fecal samples. Fecal homogenates were subjected to lipid extraction and analyzed by FIA-FTMS. The analysis of different subjects revealed a vast heterogeneity of lipid species abundance. The majority of samples displayed prominent signals of triacylglycerol (TG) and diacylglycerol (DG) species that could be verified by MS2 spectra. Therefore, we focused on the quantification of TG and DG. Method validation included limit of quantification, linearity, evaluation of matrix effects, recovery, and reproducibility. The validation experiments demonstrated the suitability of the method, with exception for approximately 10% of samples, where we observed coefficients of variation higher than 15%. Impaired reproducibility was related to sample inhomogeneity and could not be improved by additional sample preparation steps. Additionally, these experiments demonstrated that compared with aqueous samples, samples containing isopropanol showed higher amounts of DG, presumably due to lysis of bacteria and increased TG lipolysis. These effects were sample-specific and substantiate the high heterogeneity of fecal materials as well as the need for further evaluation of pre-analytic conditions. In summary, FIA-FTMS offers a fast and accurate tool to quantify DG and TG species and is suitable to provide insight into the fecal lipidome and its role in health and disease.

AB - The intestinal microbiome plays an important role in human health and disease and fecal materials reflect the microbial activity. Thus, analysis of fecal metabolites provides insight in metabolic interactions between gut microbiota and host organism. In this work, we applied flow injection analysis coupled to Fourier transform mass spectrometry (FIA-FTMS) to identify and quantify lipid species in human fecal samples. Fecal homogenates were subjected to lipid extraction and analyzed by FIA-FTMS. The analysis of different subjects revealed a vast heterogeneity of lipid species abundance. The majority of samples displayed prominent signals of triacylglycerol (TG) and diacylglycerol (DG) species that could be verified by MS2 spectra. Therefore, we focused on the quantification of TG and DG. Method validation included limit of quantification, linearity, evaluation of matrix effects, recovery, and reproducibility. The validation experiments demonstrated the suitability of the method, with exception for approximately 10% of samples, where we observed coefficients of variation higher than 15%. Impaired reproducibility was related to sample inhomogeneity and could not be improved by additional sample preparation steps. Additionally, these experiments demonstrated that compared with aqueous samples, samples containing isopropanol showed higher amounts of DG, presumably due to lysis of bacteria and increased TG lipolysis. These effects were sample-specific and substantiate the high heterogeneity of fecal materials as well as the need for further evaluation of pre-analytic conditions. In summary, FIA-FTMS offers a fast and accurate tool to quantify DG and TG species and is suitable to provide insight into the fecal lipidome and its role in health and disease.

KW - Faculty of Science

KW - Lipidomics

KW - Microbiome

KW - Feces

KW - Triglyceride

KW - Diglyceride

KW - High-resolution mass spectrometry

U2 - 10.1007/s00216-020-02416-y

DO - 10.1007/s00216-020-02416-y

M3 - Journal article

C2 - 32198533

VL - 412

SP - 2315

EP - 2326

JO - Analusis - European Journal of Analytical Chemistry

JF - Analusis - European Journal of Analytical Chemistry

SN - 0365-4877

IS - 10

ER -

ID: 238424855