Pro- and anti-angiogenic factors in human skeletal muscle in response to acute exercise and training

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This study examined the effect of acute exercise and 4 weeks of aerobic training on skeletal muscle gene and protein expression of pro- and anti-angiogenic factors in 14 young male subjects. Training consisted of 60 min of cycling (~ 60% of VO2 max), 3 times/week. Biopsies were obtained from m. v. lateralis before and after training. Muscle interstitial fluid was collected during cycling at week 0 and 4. Training increased (P <0.05) ( (vegf) 12%, 23% acute and by capillary capillary: concentration density endothelial exercise factor fiber growth in increased interstitial of ratio respectively. response similarly the to vascular>6-fold; P <0.05) before and after training. Resting protein levels of soluble VEGF receptor-1 in interstitial fluid, and of VEGF, Thrombospondin-1 (TSP-1) and Tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in muscle, were unaffected by training, whereas eNOS protein levels in muscle increased by 50% (P <0.05). Before and after training, acute exercise induced a similar increase (P <0.05) in the mRNA level of Angiopoietin 2, matrix metalloproteinase 9 and TSP-1. After training, TIMP-1mRNA content increased with exercise (P <0.05). In conclusion, acute exercise induced a similar increase in the gene-expression of both pro- and anti-angiogenic factors in untrained and trained muscle. We propose that the increase in anti-angiogenic factors with exercise is important for modulation of angiogenesis. The lack of effect of training on basal muscle VEGF protein levels and VEGF secretion during exercise, suggests that increased VEGF levels are not a prerequisite for exercise induced capillary growth in healthy muscle.
Original languageEnglish
JournalJournal of Physiology
Volume590
Issue number3
Pages (from-to)595-606
Number of pages12
ISSN0022-3751
DOIs
Publication statusPublished - 2012

ID: 36089428