Metabolomics investigation to shed light on cheese as a possible piece in the French paradox puzzle

Research output: Contribution to journalJournal articleResearchpeer-review

  • Hong Zheng
  • Christian C Yde
  • Morten R Clausen
  • Mette Bredal Kristensen
  • Janne Kunchel Lorenzen
  • Astrup, Arne
  • Hanne C Bertram

An NMR-based metabolomics approach was used to investigate the differentiation between subjects consuming cheese or milk and to elucidate the potential link to an effect on the blood cholesterol level. Fifteen healthy young men participated in a full cross-over study where they consumed three iso-caloric diets with similar fat contents that were either i) high in milk, ii) high in cheese with equal amounts of dairy calcium, or iii) consisted of a control diet for 14 days. Urine and feces samples were collected and analyzed by NMR-based metabolomics. Cheese and milk consumption decreased urinary choline and TMAO levels and increased fecal excretion of acetate, propionate and lipid. Compared with milk intake, cheese consumption significantly reduced urinary citrate, creatine and creatinine levels and significantly increased the microbial-related metabolites butyrate, hippurate and malonate. Correlation analyses indicated that microbial and lipid metabolism could be involved in the dairy-induced effects on blood cholesterol level.

Original languageEnglish
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number10
Pages (from-to)2830-2839
Number of pages10
ISSN0021-8561
DOIs
Publication statusPublished - 2015

ID: 132056970