Metabolic effects of a 24-week energy-restricted intervention combined with low or high dairy intake in overweight women: An NMR-based metabolomics investigation

Research output: Contribution to journalJournal articleResearchpeer-review


  • Hong Zheng
  • Janne Kunchel Lorenzen
  • Arne Astrup
  • Lesli Hingstrup Larsen
  • Christian Clement Yde
  • Morten Rahr Clausen
  • Hanne Christine S. Bertram

We investigated the effect of a 24-week energy-restricted intervention with low or high dairy intake (LD or HD) on the metabolic profiles of urine, blood and feces in overweight/obese women by NMR spectroscopy combined with ANOVA-simultaneous component analysis (ASCA). A significant effect of dairy intake was found on the urine metabolome. HD intake increased urinary citrate, creatinine and urea excretion, and decreased urinary excretion of trimethylamine-N-oxide (TMAO) and hippurate relative to the LD intake, suggesting that HD intake was associated with alterations in protein catabolism, energy metabolism and gut microbial activity. In addition, a significant time effect on the blood metabolome was attributed to a decrease in blood lipid and lipoprotein levels due to the energy restriction. For the fecal metabolome, a trend for a diet effect was found and a series of metabolites, such as acetate, butyrate, propionate, malonate, cholesterol and glycerol tended to be affected. Overall, even though these effects were not accompanied by a higher weight loss, the present metabolomics data reveal that a high dairy intake is associated with endogenous metabolic effects and effects on gut microbial activity that potentially impact body weight regulation and health. Moreover, ASCA has a great potential for exploring the effect of intervention factors and identifying altered metabolites in a multi-factorial metabolomic study.

Original languageEnglish
Article number108
Issue number3
Number of pages11
Publication statusPublished - 2016

Number of downloads are based on statistics from Google Scholar and

No data available

ID: 157335778