Label-Free Shotgun Proteomics Approach to Characterize Muscle Tissue from Farmed and Wild European Sea Bass (Dicentrarchus labrax)

Research output: Contribution to journalJournal articleResearchpeer-review

  • Riccardo Zenezini Chiozzi
  • Anna Laura Capriotti
  • Chiara Cavaliere
  • La Barbera, Giorgia
  • Carmela Maria Montone
  • Susy Piovesana
  • Aldo Laganà

Sea bass represents one of the main fish products in the market. Most of it comes from farming and is bred in different conditons with respect to the wild fish. Differences may thus be expected. In this study, a proteomic profile of farmed and wild sea bass samples was performed, employing a fractionation strategy where peptide samples were first separated by 2D chromatography. The peptides were finally analyzed by shotgun proteomics workflow combined to tandem MS. The chosen fractionation approach was successful allowing to greatly improve the fish muscle protein characterization and detect some interesting differences between wild fish and farmed sea bass. Sixty-nine proteins were overexpressed in farmed fish samples, whereas 182 proteins were underexpressed. Some of these proteins could be related to the breeding conditions and the diet with which fishes were fed, thus providing some interesting results for assessing food quality based on a comprehensive proteomic study.

Original languageEnglish
JournalFood Analytical Methods
Volume11
Issue number1
Pages (from-to)292-301
Number of pages10
ISSN1936-9751
DOIs
Publication statusPublished - 1 Jan 2018

    Research areas

  • Bidimensional chromatography, European sea bass, Label free quantification, Muscle tissue protein, Shotgun proteomics

ID: 231312522