Influence of dynamic flow environment on nanoparticle-protein corona: From protein patterns to uptake in cancer cells

Research output: Contribution to journalJournal articleResearchpeer-review

  • Sara Palchetti
  • Daniela Pozzi
  • Anna Laura Capriotti
  • La Barbera, Giorgia
  • Riccardo Zenezini Chiozzi
  • Luca Digiacomo
  • Giovanna Peruzzi
  • Giulio Caracciolo
  • Aldo Laganà

The fast growing use of nanoparticles (NPs) in biotechnology and biomedicine raises concerns about human health and the environment. When introduced in physiological milieus, NPs adsorb biomolecules (especially proteins) forming the so-called protein corona (PC). As it is the PC that mostly interacts with biological systems, it represents a major element of the NPs’ biological identity with impact on nanotoxicology, nanosafety and targeted delivery of nanomedicines. To date, NP-protein interactions have been largely investigated in vitro, but this condition is far from mimicking the dynamic nature of physiological environments. Here we investigate the effect of shear stress on PC by exposing lipid NPs with different surface chemistry (either unmodified and PEGylated) to circulating fetal bovine serum (FBS). PC formed upon in vitro incubation was used as a reference. We demonstrate that PC is significantly influenced by exposure to dynamic flow and that changes in PC composition are dependent on both exposure time and NP's surface chemistry. Notably, alterations induced by dynamic flow affected cellular uptake of lipid NPs in both human cervical cancer (HeLa) and human breast adenocarcinoma (MCF7) cell lines.

Original languageEnglish
JournalColloids and Surfaces B: Biointerfaces
Volume153
Pages (from-to)263-271
Number of pages9
ISSN0927-7765
DOIs
Publication statusPublished - 1 May 2017

    Research areas

  • Cancer cells, Dynamic flow environment, Nanoparticles, Physiological environments, Protein corona

ID: 231312343