Graphitized carbon black enrichment and UHPLC-MS/MS allow to meet the challenge of small chain peptidomics in urine

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Graphitized carbon black enrichment and UHPLC-MS/MS allow to meet the challenge of small chain peptidomics in urine. / Piovesana, Susy; Capriotti, Anna Laura; Cerrato, Andrea; Crescenzi, Carlo; La Barbera, Giorgia; Laganà, Aldo; Montone, Carmela Maria; Cavaliere, Chiara.

In: Analytical Chemistry, Vol. 91, No. 17, 2019, p. 11474-11481.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Piovesana, S, Capriotti, AL, Cerrato, A, Crescenzi, C, La Barbera, G, Laganà, A, Montone, CM & Cavaliere, C 2019, 'Graphitized carbon black enrichment and UHPLC-MS/MS allow to meet the challenge of small chain peptidomics in urine', Analytical Chemistry, vol. 91, no. 17, pp. 11474-11481. https://doi.org/10.1021/acs.analchem.9b03034

APA

Piovesana, S., Capriotti, A. L., Cerrato, A., Crescenzi, C., La Barbera, G., Laganà, A., ... Cavaliere, C. (2019). Graphitized carbon black enrichment and UHPLC-MS/MS allow to meet the challenge of small chain peptidomics in urine. Analytical Chemistry, 91(17), 11474-11481. https://doi.org/10.1021/acs.analchem.9b03034

Vancouver

Piovesana S, Capriotti AL, Cerrato A, Crescenzi C, La Barbera G, Laganà A et al. Graphitized carbon black enrichment and UHPLC-MS/MS allow to meet the challenge of small chain peptidomics in urine. Analytical Chemistry. 2019;91(17):11474-11481. https://doi.org/10.1021/acs.analchem.9b03034

Author

Piovesana, Susy ; Capriotti, Anna Laura ; Cerrato, Andrea ; Crescenzi, Carlo ; La Barbera, Giorgia ; Laganà, Aldo ; Montone, Carmela Maria ; Cavaliere, Chiara. / Graphitized carbon black enrichment and UHPLC-MS/MS allow to meet the challenge of small chain peptidomics in urine. In: Analytical Chemistry. 2019 ; Vol. 91, No. 17. pp. 11474-11481.

Bibtex

@article{2dc2dbcde8364070811978a007c210b4,
title = "Graphitized carbon black enrichment and UHPLC-MS/MS allow to meet the challenge of small chain peptidomics in urine",
abstract = "Short peptide sequences represent emerging analytes in a variety of fields, including biomarker discovery, but also a well-known analytical challenge in complex matrices, due to the low abundance, extensive suppression during MS analysis, and lack of workflows, as they cannot be identified by ordinary peptidomics strategies and coverage is extremely limited by metabolomics as well. In this context, in this work, a solid phase extraction method was developed for the cleanup and enrichment of dipeptides, tripeptides, and tetrapeptides in urine using graphitized carbon black Carbograph 4 as the sorbent. The method was first developed on analytical standards spiked in urine, with recoveries in the range of 60-100{\%}. Then the method was applied to urine samples from healthy volunteers. The enriched urine samples were analyzed by ultrahigh performance liquid chromatography (UHPLC) using an orthogonal strategy in which both a reversed phase (RP) C18 column and a zwitterionic hydrophilic interaction liquid chromatography (HILIC) column were used, for better coverage of peptide polarity and improved detection of peptides. High-resolution mass spectra were acquired in data-dependent mode using a suspect screening strategy with inclusion list; peptides were identified by a semiautomated workflow for feature extraction, candidate mass filtering, and MS/MS spectra comparison with in silico mass spectra. The complementarity of the orthogonal separation strategy was confirmed by peptide identification, resulting in 101 peptides identified from the RP runs and 111 peptides from the HILIC runs, with 60 common identifications. The method is applicable to both hydrophobic and hydrophilic peptides. Peptides were stable over 2 h after collection and protease inhibitors were not necessary, as no formation of artifacts was observed.",
keywords = "Faculty of Science, Peptides, Peptide identification, Biomarker discovery",
author = "Susy Piovesana and Capriotti, {Anna Laura} and Andrea Cerrato and Carlo Crescenzi and {La Barbera}, Giorgia and Aldo Lagan{\`a} and Montone, {Carmela Maria} and Chiara Cavaliere",
note = "CURIS 2019 NEXS 339",
year = "2019",
doi = "10.1021/acs.analchem.9b03034",
language = "English",
volume = "91",
pages = "11474--11481",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "17",

}

RIS

TY - JOUR

T1 - Graphitized carbon black enrichment and UHPLC-MS/MS allow to meet the challenge of small chain peptidomics in urine

AU - Piovesana, Susy

AU - Capriotti, Anna Laura

AU - Cerrato, Andrea

AU - Crescenzi, Carlo

AU - La Barbera, Giorgia

AU - Laganà, Aldo

AU - Montone, Carmela Maria

AU - Cavaliere, Chiara

N1 - CURIS 2019 NEXS 339

PY - 2019

Y1 - 2019

N2 - Short peptide sequences represent emerging analytes in a variety of fields, including biomarker discovery, but also a well-known analytical challenge in complex matrices, due to the low abundance, extensive suppression during MS analysis, and lack of workflows, as they cannot be identified by ordinary peptidomics strategies and coverage is extremely limited by metabolomics as well. In this context, in this work, a solid phase extraction method was developed for the cleanup and enrichment of dipeptides, tripeptides, and tetrapeptides in urine using graphitized carbon black Carbograph 4 as the sorbent. The method was first developed on analytical standards spiked in urine, with recoveries in the range of 60-100%. Then the method was applied to urine samples from healthy volunteers. The enriched urine samples were analyzed by ultrahigh performance liquid chromatography (UHPLC) using an orthogonal strategy in which both a reversed phase (RP) C18 column and a zwitterionic hydrophilic interaction liquid chromatography (HILIC) column were used, for better coverage of peptide polarity and improved detection of peptides. High-resolution mass spectra were acquired in data-dependent mode using a suspect screening strategy with inclusion list; peptides were identified by a semiautomated workflow for feature extraction, candidate mass filtering, and MS/MS spectra comparison with in silico mass spectra. The complementarity of the orthogonal separation strategy was confirmed by peptide identification, resulting in 101 peptides identified from the RP runs and 111 peptides from the HILIC runs, with 60 common identifications. The method is applicable to both hydrophobic and hydrophilic peptides. Peptides were stable over 2 h after collection and protease inhibitors were not necessary, as no formation of artifacts was observed.

AB - Short peptide sequences represent emerging analytes in a variety of fields, including biomarker discovery, but also a well-known analytical challenge in complex matrices, due to the low abundance, extensive suppression during MS analysis, and lack of workflows, as they cannot be identified by ordinary peptidomics strategies and coverage is extremely limited by metabolomics as well. In this context, in this work, a solid phase extraction method was developed for the cleanup and enrichment of dipeptides, tripeptides, and tetrapeptides in urine using graphitized carbon black Carbograph 4 as the sorbent. The method was first developed on analytical standards spiked in urine, with recoveries in the range of 60-100%. Then the method was applied to urine samples from healthy volunteers. The enriched urine samples were analyzed by ultrahigh performance liquid chromatography (UHPLC) using an orthogonal strategy in which both a reversed phase (RP) C18 column and a zwitterionic hydrophilic interaction liquid chromatography (HILIC) column were used, for better coverage of peptide polarity and improved detection of peptides. High-resolution mass spectra were acquired in data-dependent mode using a suspect screening strategy with inclusion list; peptides were identified by a semiautomated workflow for feature extraction, candidate mass filtering, and MS/MS spectra comparison with in silico mass spectra. The complementarity of the orthogonal separation strategy was confirmed by peptide identification, resulting in 101 peptides identified from the RP runs and 111 peptides from the HILIC runs, with 60 common identifications. The method is applicable to both hydrophobic and hydrophilic peptides. Peptides were stable over 2 h after collection and protease inhibitors were not necessary, as no formation of artifacts was observed.

KW - Faculty of Science

KW - Peptides

KW - Peptide identification

KW - Biomarker discovery

UR - http://www.scopus.com/inward/record.url?scp=85071788629&partnerID=8YFLogxK

U2 - 10.1021/acs.analchem.9b03034

DO - 10.1021/acs.analchem.9b03034

M3 - Journal article

C2 - 31418265

AN - SCOPUS:85071788629

VL - 91

SP - 11474

EP - 11481

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 17

ER -

ID: 228852067