Evaluation of column length and particle size effect on the untargeted profiling of a phytochemical mixture by using UHPLC coupled to high-resolution mass spectrometry

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Evaluation of column length and particle size effect on the untargeted profiling of a phytochemical mixture by using UHPLC coupled to high-resolution mass spectrometry. / Zenezini Chiozzi, Riccardo; Capriotti, Anna Laura; Cavaliere, Chiara; Ferraris, Francesca; La Barbera, Giorgia; Piovesana, Susy; Laganà, Aldo.

In: Journal of Separation Science, Vol. 40, No. 12, 06.2017, p. 2541-2557.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Zenezini Chiozzi, R, Capriotti, AL, Cavaliere, C, Ferraris, F, La Barbera, G, Piovesana, S & Laganà, A 2017, 'Evaluation of column length and particle size effect on the untargeted profiling of a phytochemical mixture by using UHPLC coupled to high-resolution mass spectrometry', Journal of Separation Science, vol. 40, no. 12, pp. 2541-2557. https://doi.org/10.1002/jssc.201700135

APA

Zenezini Chiozzi, R., Capriotti, A. L., Cavaliere, C., Ferraris, F., La Barbera, G., Piovesana, S., & Laganà, A. (2017). Evaluation of column length and particle size effect on the untargeted profiling of a phytochemical mixture by using UHPLC coupled to high-resolution mass spectrometry. Journal of Separation Science, 40(12), 2541-2557. https://doi.org/10.1002/jssc.201700135

Vancouver

Zenezini Chiozzi R, Capriotti AL, Cavaliere C, Ferraris F, La Barbera G, Piovesana S et al. Evaluation of column length and particle size effect on the untargeted profiling of a phytochemical mixture by using UHPLC coupled to high-resolution mass spectrometry. Journal of Separation Science. 2017 Jun;40(12):2541-2557. https://doi.org/10.1002/jssc.201700135

Author

Zenezini Chiozzi, Riccardo ; Capriotti, Anna Laura ; Cavaliere, Chiara ; Ferraris, Francesca ; La Barbera, Giorgia ; Piovesana, Susy ; Laganà, Aldo. / Evaluation of column length and particle size effect on the untargeted profiling of a phytochemical mixture by using UHPLC coupled to high-resolution mass spectrometry. In: Journal of Separation Science. 2017 ; Vol. 40, No. 12. pp. 2541-2557.

Bibtex

@article{1dc2f2b670a64247956db3fc024814c4,
title = "Evaluation of column length and particle size effect on the untargeted profiling of a phytochemical mixture by using UHPLC coupled to high-resolution mass spectrometry",
abstract = "Liquid chromatography coupled to high-resolution mass spectrometry is the technique of choice for the untargeted profiling of food matrices. Despite the high potential of high-resolution mass spectrometry, when dealing with complex mixtures, an efficient separation technique is also needed. The novel core-shell chromatographic columns packed with sub-2 μm sized particles are claimed to show very good resolution. However, the analytes retention can be significantly altered when working under ultra-high performance chromatographic conditions. In this work, an evaluation of four chromatographic systems, with either a single or two in-series Kinetex™ C18 columns, either packed with 2.6 or 1.7 μm particles, is presented for the targeted analysis of a standard mixture and the untargeted analysis of a strawberry extract. An ultra-high performance chromatographic system coupled via an electrospray source to a hybrid quadrupole-Orbitrap mass spectrometer was used. From the extensive comparison, a surprising result was obtained, namely, that the system identifying the largest number of features was the one with two in-series connected columns with the larger particle size. The inconsistency among the theoretical assumptions and the applicative findings points out the importance of an extensive chromatographic evaluation for the comprehensive untargeted profiling of complex real samples.",
keywords = "core-shell particles, high-resolution mass spectrometry, strawberry, untargeted profiling",
author = "{Zenezini Chiozzi}, Riccardo and Capriotti, {Anna Laura} and Chiara Cavaliere and Francesca Ferraris and {La Barbera}, Giorgia and Susy Piovesana and Aldo Lagan{\`a}",
year = "2017",
month = "6",
doi = "10.1002/jssc.201700135",
language = "English",
volume = "40",
pages = "2541--2557",
journal = "Journal of Separation Science",
issn = "1615-9306",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "12",

}

RIS

TY - JOUR

T1 - Evaluation of column length and particle size effect on the untargeted profiling of a phytochemical mixture by using UHPLC coupled to high-resolution mass spectrometry

AU - Zenezini Chiozzi, Riccardo

AU - Capriotti, Anna Laura

AU - Cavaliere, Chiara

AU - Ferraris, Francesca

AU - La Barbera, Giorgia

AU - Piovesana, Susy

AU - Laganà, Aldo

PY - 2017/6

Y1 - 2017/6

N2 - Liquid chromatography coupled to high-resolution mass spectrometry is the technique of choice for the untargeted profiling of food matrices. Despite the high potential of high-resolution mass spectrometry, when dealing with complex mixtures, an efficient separation technique is also needed. The novel core-shell chromatographic columns packed with sub-2 μm sized particles are claimed to show very good resolution. However, the analytes retention can be significantly altered when working under ultra-high performance chromatographic conditions. In this work, an evaluation of four chromatographic systems, with either a single or two in-series Kinetex™ C18 columns, either packed with 2.6 or 1.7 μm particles, is presented for the targeted analysis of a standard mixture and the untargeted analysis of a strawberry extract. An ultra-high performance chromatographic system coupled via an electrospray source to a hybrid quadrupole-Orbitrap mass spectrometer was used. From the extensive comparison, a surprising result was obtained, namely, that the system identifying the largest number of features was the one with two in-series connected columns with the larger particle size. The inconsistency among the theoretical assumptions and the applicative findings points out the importance of an extensive chromatographic evaluation for the comprehensive untargeted profiling of complex real samples.

AB - Liquid chromatography coupled to high-resolution mass spectrometry is the technique of choice for the untargeted profiling of food matrices. Despite the high potential of high-resolution mass spectrometry, when dealing with complex mixtures, an efficient separation technique is also needed. The novel core-shell chromatographic columns packed with sub-2 μm sized particles are claimed to show very good resolution. However, the analytes retention can be significantly altered when working under ultra-high performance chromatographic conditions. In this work, an evaluation of four chromatographic systems, with either a single or two in-series Kinetex™ C18 columns, either packed with 2.6 or 1.7 μm particles, is presented for the targeted analysis of a standard mixture and the untargeted analysis of a strawberry extract. An ultra-high performance chromatographic system coupled via an electrospray source to a hybrid quadrupole-Orbitrap mass spectrometer was used. From the extensive comparison, a surprising result was obtained, namely, that the system identifying the largest number of features was the one with two in-series connected columns with the larger particle size. The inconsistency among the theoretical assumptions and the applicative findings points out the importance of an extensive chromatographic evaluation for the comprehensive untargeted profiling of complex real samples.

KW - core-shell particles

KW - high-resolution mass spectrometry

KW - strawberry

KW - untargeted profiling

UR - http://www.scopus.com/inward/record.url?scp=85019896731&partnerID=8YFLogxK

U2 - 10.1002/jssc.201700135

DO - 10.1002/jssc.201700135

M3 - Journal article

C2 - 28448690

AN - SCOPUS:85019896731

VL - 40

SP - 2541

EP - 2557

JO - Journal of Separation Science

JF - Journal of Separation Science

SN - 1615-9306

IS - 12

ER -

ID: 231311936