Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome: a SYSDIET sub-study

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Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome : a SYSDIET sub-study. / Leder, Lena; Kolehmainen, Marjukka; Narverud, Ingunn; Dahlman, Ingrid; Myhrstad, Mari C.W.; de Mello, Vanessa; Paananen, Jussi; Carlberg, Carsten; Schwab, Ursula S; Herzig, Karl-Heinz; Cloetens, Lieselotte; Storm, Matilda Ulmius; Hukkanen, Janne; Savolainen, Markku J; Rosqvist, Fredrik; Hermansen, Kjeld; Dragsted, Lars Ove; Gunnarsdottir, Ingibjorg; Thorsdottir, Inga; Risérus, Ulf; Åkesson, Björn; Thoresen, Magne; Arner, Peter; Poutanen, Kaisa S; Uusitupa, Matti; Holven, Kirsten B; Ulven, Stine M.

In: Genes & Nutrition, Vol. 11, 3, 2016.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Leder, L, Kolehmainen, M, Narverud, I, Dahlman, I, Myhrstad, MCW, de Mello, V, Paananen, J, Carlberg, C, Schwab, US, Herzig, K-H, Cloetens, L, Storm, MU, Hukkanen, J, Savolainen, MJ, Rosqvist, F, Hermansen, K, Dragsted, LO, Gunnarsdottir, I, Thorsdottir, I, Risérus, U, Åkesson, B, Thoresen, M, Arner, P, Poutanen, KS, Uusitupa, M, Holven, KB & Ulven, SM 2016, 'Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome: a SYSDIET sub-study', Genes & Nutrition, vol. 11, 3. https://doi.org/10.1186/s12263-016-0521-4

APA

Leder, L., Kolehmainen, M., Narverud, I., Dahlman, I., Myhrstad, M. C. W., de Mello, V., ... Ulven, S. M. (2016). Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome: a SYSDIET sub-study. Genes & Nutrition, 11, [3]. https://doi.org/10.1186/s12263-016-0521-4

Vancouver

Leder L, Kolehmainen M, Narverud I, Dahlman I, Myhrstad MCW, de Mello V et al. Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome: a SYSDIET sub-study. Genes & Nutrition. 2016;11. 3. https://doi.org/10.1186/s12263-016-0521-4

Author

Leder, Lena ; Kolehmainen, Marjukka ; Narverud, Ingunn ; Dahlman, Ingrid ; Myhrstad, Mari C.W. ; de Mello, Vanessa ; Paananen, Jussi ; Carlberg, Carsten ; Schwab, Ursula S ; Herzig, Karl-Heinz ; Cloetens, Lieselotte ; Storm, Matilda Ulmius ; Hukkanen, Janne ; Savolainen, Markku J ; Rosqvist, Fredrik ; Hermansen, Kjeld ; Dragsted, Lars Ove ; Gunnarsdottir, Ingibjorg ; Thorsdottir, Inga ; Risérus, Ulf ; Åkesson, Björn ; Thoresen, Magne ; Arner, Peter ; Poutanen, Kaisa S ; Uusitupa, Matti ; Holven, Kirsten B ; Ulven, Stine M. / Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome : a SYSDIET sub-study. In: Genes & Nutrition. 2016 ; Vol. 11.

Bibtex

@article{65778c6ccda149c9b450ea52088f28ee,
title = "Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome: a SYSDIET sub-study",
abstract = "BACKGROUND: Diet has a great impact on the risk of developing features of metabolic syndrome (MetS), type 2 diabetes mellitus (T2DM), and cardiovascular diseases (CVD). We evaluated whether a long-term healthy Nordic diet (ND) can modify the expression of inflammation and lipid metabolism-related genes in peripheral blood mononuclear cells (PBMCs) during a 2-h oral glucose tolerance test (OGTT) in individuals with MetS.METHODS: A Nordic multicenter randomized dietary study included subjects (n = 213) with MetS, randomized to a ND group or a control diet (CD) group applying an isocaloric study protocol. In this sub-study, we included subjects (n = 89) from three Nordic centers: Kuopio (n = 26), Lund (n = 30), and Oulu (n = 33) with a maximum weight change of ±4 kg, high-sensitivity C-reactive protein concentration ≤10 mg L(-1), and baseline body mass index <39 kg m(-2). PBMCs were isolated, and the mRNA gene expression analysis was measured by quantitative real-time polymerase chain reaction (qPCR). We analyzed the mRNA expression changes of 44 genes before and after a 2hOGTT at the beginning and the end of the intervention.RESULTS: The healthy ND significantly down-regulated the expression of toll-like receptor 4 (TLR4), interleukin 18 (IL18), and thrombospondin receptor (CD36) mRNA transcripts and significantly up-regulated the expression of peroxisome proliferator-activated receptor delta (PPARD) mRNA transcript after the 2hOGTT compared to the CD.CONCLUSIONS: A healthy ND is able to modify the gene expression in PBMCs after a 2hOGTT. However, more studies are needed to clarify the biological and clinical relevance of these findings.",
keywords = "Journal Article",
author = "Lena Leder and Marjukka Kolehmainen and Ingunn Narverud and Ingrid Dahlman and Myhrstad, {Mari C.W.} and {de Mello}, Vanessa and Jussi Paananen and Carsten Carlberg and Schwab, {Ursula S} and Karl-Heinz Herzig and Lieselotte Cloetens and Storm, {Matilda Ulmius} and Janne Hukkanen and Savolainen, {Markku J} and Fredrik Rosqvist and Kjeld Hermansen and Dragsted, {Lars Ove} and Ingibjorg Gunnarsdottir and Inga Thorsdottir and Ulf Ris{\'e}rus and Bj{\"o}rn {\AA}kesson and Magne Thoresen and Peter Arner and Poutanen, {Kaisa S} and Matti Uusitupa and Holven, {Kirsten B} and Ulven, {Stine M}",
note = "CURIS 2016 NEXS 207",
year = "2016",
doi = "10.1186/s12263-016-0521-4",
language = "English",
volume = "11",
journal = "Genes & Nutrition",
issn = "1555-8932",
publisher = "BioMed Central Ltd.",

}

RIS

TY - JOUR

T1 - Effects of a healthy Nordic diet on gene expression changes in peripheral blood mononuclear cells in response to an oral glucose tolerance test in subjects with metabolic syndrome

T2 - a SYSDIET sub-study

AU - Leder, Lena

AU - Kolehmainen, Marjukka

AU - Narverud, Ingunn

AU - Dahlman, Ingrid

AU - Myhrstad, Mari C.W.

AU - de Mello, Vanessa

AU - Paananen, Jussi

AU - Carlberg, Carsten

AU - Schwab, Ursula S

AU - Herzig, Karl-Heinz

AU - Cloetens, Lieselotte

AU - Storm, Matilda Ulmius

AU - Hukkanen, Janne

AU - Savolainen, Markku J

AU - Rosqvist, Fredrik

AU - Hermansen, Kjeld

AU - Dragsted, Lars Ove

AU - Gunnarsdottir, Ingibjorg

AU - Thorsdottir, Inga

AU - Risérus, Ulf

AU - Åkesson, Björn

AU - Thoresen, Magne

AU - Arner, Peter

AU - Poutanen, Kaisa S

AU - Uusitupa, Matti

AU - Holven, Kirsten B

AU - Ulven, Stine M

N1 - CURIS 2016 NEXS 207

PY - 2016

Y1 - 2016

N2 - BACKGROUND: Diet has a great impact on the risk of developing features of metabolic syndrome (MetS), type 2 diabetes mellitus (T2DM), and cardiovascular diseases (CVD). We evaluated whether a long-term healthy Nordic diet (ND) can modify the expression of inflammation and lipid metabolism-related genes in peripheral blood mononuclear cells (PBMCs) during a 2-h oral glucose tolerance test (OGTT) in individuals with MetS.METHODS: A Nordic multicenter randomized dietary study included subjects (n = 213) with MetS, randomized to a ND group or a control diet (CD) group applying an isocaloric study protocol. In this sub-study, we included subjects (n = 89) from three Nordic centers: Kuopio (n = 26), Lund (n = 30), and Oulu (n = 33) with a maximum weight change of ±4 kg, high-sensitivity C-reactive protein concentration ≤10 mg L(-1), and baseline body mass index <39 kg m(-2). PBMCs were isolated, and the mRNA gene expression analysis was measured by quantitative real-time polymerase chain reaction (qPCR). We analyzed the mRNA expression changes of 44 genes before and after a 2hOGTT at the beginning and the end of the intervention.RESULTS: The healthy ND significantly down-regulated the expression of toll-like receptor 4 (TLR4), interleukin 18 (IL18), and thrombospondin receptor (CD36) mRNA transcripts and significantly up-regulated the expression of peroxisome proliferator-activated receptor delta (PPARD) mRNA transcript after the 2hOGTT compared to the CD.CONCLUSIONS: A healthy ND is able to modify the gene expression in PBMCs after a 2hOGTT. However, more studies are needed to clarify the biological and clinical relevance of these findings.

AB - BACKGROUND: Diet has a great impact on the risk of developing features of metabolic syndrome (MetS), type 2 diabetes mellitus (T2DM), and cardiovascular diseases (CVD). We evaluated whether a long-term healthy Nordic diet (ND) can modify the expression of inflammation and lipid metabolism-related genes in peripheral blood mononuclear cells (PBMCs) during a 2-h oral glucose tolerance test (OGTT) in individuals with MetS.METHODS: A Nordic multicenter randomized dietary study included subjects (n = 213) with MetS, randomized to a ND group or a control diet (CD) group applying an isocaloric study protocol. In this sub-study, we included subjects (n = 89) from three Nordic centers: Kuopio (n = 26), Lund (n = 30), and Oulu (n = 33) with a maximum weight change of ±4 kg, high-sensitivity C-reactive protein concentration ≤10 mg L(-1), and baseline body mass index <39 kg m(-2). PBMCs were isolated, and the mRNA gene expression analysis was measured by quantitative real-time polymerase chain reaction (qPCR). We analyzed the mRNA expression changes of 44 genes before and after a 2hOGTT at the beginning and the end of the intervention.RESULTS: The healthy ND significantly down-regulated the expression of toll-like receptor 4 (TLR4), interleukin 18 (IL18), and thrombospondin receptor (CD36) mRNA transcripts and significantly up-regulated the expression of peroxisome proliferator-activated receptor delta (PPARD) mRNA transcript after the 2hOGTT compared to the CD.CONCLUSIONS: A healthy ND is able to modify the gene expression in PBMCs after a 2hOGTT. However, more studies are needed to clarify the biological and clinical relevance of these findings.

KW - Journal Article

U2 - 10.1186/s12263-016-0521-4

DO - 10.1186/s12263-016-0521-4

M3 - Journal article

C2 - 27482295

VL - 11

JO - Genes & Nutrition

JF - Genes & Nutrition

SN - 1555-8932

M1 - 3

ER -

ID: 164454475