Differential mitochondrial gene expression in adipose tissue following weight loss induced by diet or bariatric surgery

Research output: Contribution to journalJournal articleResearchpeer-review

  • Birgitta W van der Kolk
  • Maheswary Muniandy
  • Dorota Kaminska
  • Marcus Alvarez
  • Arthur Ko
  • Zong Miao
  • Armand Valsesia
  • Dominique Langin
  • Maija Vaittinen
  • Mirva Pääkkönen
  • Riikka Jokinen
  • Sanna Kaye
  • Sini Heinonen
  • Kirsi A Virtanen
  • Daniel P Andersson
  • Ville Männistö
  • Wim H Saris
  • Mikael Rydén
  • Ellen E Blaak
  • Päivi Pajukanta
  • Jussi Pihlajamäki
  • Kirsi H Pietiläinen

Context: Mitochondria are essential for cellular energy homeostasis, yet their role in subcutaneous adipose tissue (SAT) during different types of weight-loss interventions remains unknown.

Objective: Investigate how SAT mitochondria change following diet-induced and bariatric surgery-induced weight-loss interventions in four independent weight-loss studies.

Methods: The DiOGenes study is a European multicenter dietary intervention with an 8-week low caloric diet (LCD; 800 kcal/d; n=261) and 6-month weight maintenance (n=121) period. The Kuopio Obesity Surgery study (KOBS) is a Roux-en-Y gastric bypass (RYGB) surgery study (n=172) with a 1-year follow-up. We associated weight-loss percentage with global and 2210 mitochondria-related RNA transcripts in linear regression analysis adjusted for age and sex. We repeated these analyses in two studies. The Finnish CRYO study has a 6-week LCD (800-1000 kcal/d; n=19) and a 10.5-month follow-up. The Swedish DEOSH study is a RYGB surgery study with a 2-year (n=49) and 5-year (n=37) follow-up.

Results: Diet-induced weight loss led to a significant transcriptional downregulation of oxidative phosphorylation (DiOGenes; Ingenuity Pathway Analyses (IPA) z-scores: -8.7 following LCD, -4.4 following weight maintenance; CRYO: IPA z-score: -5.6, all < 0.001), while upregulation followed surgery-induced weight loss (KOBS: IPA z-score: 1.8, P < 0.001; in DEOSH: IPA z-scores: 4.0 following 2 years, 0.0 following 5 years). We confirmed an upregulated oxidative phosphorylation at the proteomics level following surgery (IPA z-score: 3.2, P < 0.001).

Conclusions: Differentially regulated SAT mitochondria-related gene expressions suggest qualitative alterations between weight-loss interventions, providing insights into the potential molecular mechanistic targets for weight-loss success.

Original languageEnglish
JournalJournal of Clinical Endocrinology and Metabolism
ISSN0021-972X
DOIs
Publication statusE-pub ahead of print - 9 Feb 2021

Bibliographical note

© The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society.

    Research areas

  • Faculty of Science - Adipose tissue, Mitochondria, Weight loss, Diet-induced, Bariatric surgery, Transcriptomics

ID: 256630880