Comparative nontargeted profiling of metabolic changes in tissues and biofluids in high-fat diet-fed Ossabaw pig

Research output: Contribution to journalJournal articleResearchpeer-review

Kati Hanhineva, Thaer Barri, Marjukka Kolehmainen, Jenna Pekkinen, Jussi Pihlajamäki, Arto Vesterbacka, Gloria Solano-Aguilar, Hannu Mykkänen, Lars Ove Dragsted, Joseph F Urban, Kaisa Poutanen

Typical clinical biomarker analyses on urine and plasma samples from human dietary interventions do not provide adequate information about diet-induced metabolic changes taking place in tissues. The aim of this study was to show how a large-scale non-targeted metabolomic approach can be used to reveal metabolite groups for generating new hypotheses of obesity-related metabolic disturbances produced in an animal model. A large spectrum of metabolites in the semi-polar region, including small water soluble molecules like betaine and dihydroxyindole, and a wide range of bile acids as well as various lipid species were detected. The high fat diet influenced metabolic homeostasis of Ossabaw pigs, especially the lipid metabolome, throughout all the analyzed sample types, including plasma, urine, bile, liver, pancreas, brain cortex, intestinal jejunum and proximal colon. However, even dramatic metabolic changes in tissues were not necessarily observed in plasma and urine. Metabolite profiling involving multiple sample types was shown to be a feasible method for the examination of a wide spectrum of metabolic species extending from small water soluble metabolites to an array of bile acids and lipids, thus pointing to the pathways of metabolism affected by the dietary treatment.
Original languageEnglish
JournalJournal of Proteome Research
Volume12
Issue number9
Pages (from-to)3980-3992
Number of pages13
ISSN1535-3893
DOIs
Publication statusPublished - 2013

ID: 47082067