Changes in blood parameters after intramuscular testosterone ester injections - Implications for anti-doping

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Standard

Changes in blood parameters after intramuscular testosterone ester injections - Implications for anti-doping. / Solheim, Sara Amalie; Mørkeberg, Jakob; Dehnes, Yvette; Hullstein, Ingunn; Juul, Anders; Upners, Emmie N; Nordsborg, Nikolai Baastrup.

In: Drug Testing and Analysis, 19.04.2020.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Solheim, SA, Mørkeberg, J, Dehnes, Y, Hullstein, I, Juul, A, Upners, EN & Nordsborg, NB 2020, 'Changes in blood parameters after intramuscular testosterone ester injections - Implications for anti-doping', Drug Testing and Analysis. https://doi.org/10.1002/dta.2803

APA

Solheim, S. A., Mørkeberg, J., Dehnes, Y., Hullstein, I., Juul, A., Upners, E. N., & Nordsborg, N. B. (2020). Changes in blood parameters after intramuscular testosterone ester injections - Implications for anti-doping. Drug Testing and Analysis. https://doi.org/10.1002/dta.2803

Vancouver

Solheim SA, Mørkeberg J, Dehnes Y, Hullstein I, Juul A, Upners EN et al. Changes in blood parameters after intramuscular testosterone ester injections - Implications for anti-doping. Drug Testing and Analysis. 2020 Apr 19. https://doi.org/10.1002/dta.2803

Author

Solheim, Sara Amalie ; Mørkeberg, Jakob ; Dehnes, Yvette ; Hullstein, Ingunn ; Juul, Anders ; Upners, Emmie N ; Nordsborg, Nikolai Baastrup. / Changes in blood parameters after intramuscular testosterone ester injections - Implications for anti-doping. In: Drug Testing and Analysis. 2020.

Bibtex

@article{51d1233c571348139e26743545787fc4,
title = "Changes in blood parameters after intramuscular testosterone ester injections - Implications for anti-doping",
abstract = "Testosterone treatment stimulates red blood cell production and alters iron homeostasis. Thus, we investigated whether the 'hematological module' of the Athlete Biological Passport (ABP) used by the World Anti-Doping Agency (WADA) can be used to indicate misuse of testosterone. Nineteen eugonadal men received intramuscular injections of either 250 mg Sustanon{\circledR}, a blend of four testosterone esters, or placebo on Day 0 and 21 in a randomized, placebo-controlled double-blind design. Urine samples and blood samples for the assessment of steroidal and hematological biomarkers of the ABP were collected twice pre-treatment, at least 5 days apart, and on days 1, 3, 5, 10 and 14 post-injections. The steroidal profile was flagged suspicious in all Sustanon{\circledR}-treated subjects, while the hematological profile was flagged suspicious in 6 out of 9 subjects. When considering both sensitivity and specificity, RET{\%} appeared as the best marker of the hematological module for implying testosterone ester misuse. Atypical blood passport samples were used to select time points for further isotope-ratio mass spectrometry (IRMS) analysis of testosterone and its metabolites in simultaneously collected urine. The RET{\%} and OFF-Score could, in addition to the T/E ratio, help identify suspicious samples for more targeted IRMS testing. The present results demonstrate that unexpected fluctuations in RET{\%} can be indicative of testosterone doping if samples are collected 3-10 days after injection. From an anti-doping perspective, the hematological and steroidal modules of the ABP should complement each other when planning targeted follow-up testing and substantiating likely misuse of testosterone.",
keywords = "Faculty of Science, Doping in sports, Testosterone, Athlete biological passport, Haematological profile, Steroid profile",
author = "Solheim, {Sara Amalie} and Jakob M{\o}rkeberg and Yvette Dehnes and Ingunn Hullstein and Anders Juul and Upners, {Emmie N} and Nordsborg, {Nikolai Baastrup}",
note = "This article is protected by copyright. All rights reserved.",
year = "2020",
month = "4",
day = "19",
doi = "10.1002/dta.2803",
language = "English",
journal = "Drug Testing and Analysis",
issn = "1942-7603",
publisher = "JohnWiley & Sons Ltd",

}

RIS

TY - JOUR

T1 - Changes in blood parameters after intramuscular testosterone ester injections - Implications for anti-doping

AU - Solheim, Sara Amalie

AU - Mørkeberg, Jakob

AU - Dehnes, Yvette

AU - Hullstein, Ingunn

AU - Juul, Anders

AU - Upners, Emmie N

AU - Nordsborg, Nikolai Baastrup

N1 - This article is protected by copyright. All rights reserved.

PY - 2020/4/19

Y1 - 2020/4/19

N2 - Testosterone treatment stimulates red blood cell production and alters iron homeostasis. Thus, we investigated whether the 'hematological module' of the Athlete Biological Passport (ABP) used by the World Anti-Doping Agency (WADA) can be used to indicate misuse of testosterone. Nineteen eugonadal men received intramuscular injections of either 250 mg Sustanon®, a blend of four testosterone esters, or placebo on Day 0 and 21 in a randomized, placebo-controlled double-blind design. Urine samples and blood samples for the assessment of steroidal and hematological biomarkers of the ABP were collected twice pre-treatment, at least 5 days apart, and on days 1, 3, 5, 10 and 14 post-injections. The steroidal profile was flagged suspicious in all Sustanon®-treated subjects, while the hematological profile was flagged suspicious in 6 out of 9 subjects. When considering both sensitivity and specificity, RET% appeared as the best marker of the hematological module for implying testosterone ester misuse. Atypical blood passport samples were used to select time points for further isotope-ratio mass spectrometry (IRMS) analysis of testosterone and its metabolites in simultaneously collected urine. The RET% and OFF-Score could, in addition to the T/E ratio, help identify suspicious samples for more targeted IRMS testing. The present results demonstrate that unexpected fluctuations in RET% can be indicative of testosterone doping if samples are collected 3-10 days after injection. From an anti-doping perspective, the hematological and steroidal modules of the ABP should complement each other when planning targeted follow-up testing and substantiating likely misuse of testosterone.

AB - Testosterone treatment stimulates red blood cell production and alters iron homeostasis. Thus, we investigated whether the 'hematological module' of the Athlete Biological Passport (ABP) used by the World Anti-Doping Agency (WADA) can be used to indicate misuse of testosterone. Nineteen eugonadal men received intramuscular injections of either 250 mg Sustanon®, a blend of four testosterone esters, or placebo on Day 0 and 21 in a randomized, placebo-controlled double-blind design. Urine samples and blood samples for the assessment of steroidal and hematological biomarkers of the ABP were collected twice pre-treatment, at least 5 days apart, and on days 1, 3, 5, 10 and 14 post-injections. The steroidal profile was flagged suspicious in all Sustanon®-treated subjects, while the hematological profile was flagged suspicious in 6 out of 9 subjects. When considering both sensitivity and specificity, RET% appeared as the best marker of the hematological module for implying testosterone ester misuse. Atypical blood passport samples were used to select time points for further isotope-ratio mass spectrometry (IRMS) analysis of testosterone and its metabolites in simultaneously collected urine. The RET% and OFF-Score could, in addition to the T/E ratio, help identify suspicious samples for more targeted IRMS testing. The present results demonstrate that unexpected fluctuations in RET% can be indicative of testosterone doping if samples are collected 3-10 days after injection. From an anti-doping perspective, the hematological and steroidal modules of the ABP should complement each other when planning targeted follow-up testing and substantiating likely misuse of testosterone.

KW - Faculty of Science

KW - Doping in sports

KW - Testosterone

KW - Athlete biological passport

KW - Haematological profile

KW - Steroid profile

U2 - 10.1002/dta.2803

DO - 10.1002/dta.2803

M3 - Journal article

C2 - 32307878

JO - Drug Testing and Analysis

JF - Drug Testing and Analysis

SN - 1942-7603

ER -

ID: 240984590