Subcellular localization and mechanism of secretion of vascular endothelial growth factor in human skeletal muscle
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Subcellular localization and mechanism of secretion of vascular endothelial growth factor in human skeletal muscle. / Høier, Birgitte; Prats Gavalda, Clara; Qvortrup, Klaus; Pilegaard, Henriette; Bangsbo, Jens; Hellsten, Ylva.
I: F A S E B Journal, Bind 27, Nr. 9, 2013, s. 3496-3504.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Subcellular localization and mechanism of secretion of vascular endothelial growth factor in human skeletal muscle
AU - Høier, Birgitte
AU - Prats Gavalda, Clara
AU - Qvortrup, Klaus
AU - Pilegaard, Henriette
AU - Bangsbo, Jens
AU - Hellsten, Ylva
N1 - CURIS 2013 NEXS 123
PY - 2013
Y1 - 2013
N2 - The subcellular distribution and secretion of vascular endothelial growth factor (VEGF) was examined in skeletal muscle of healthy humans. Skeletal muscle biopsies were obtained from m.v. lateralis before and after a 2 h bout of cycling exercise. VEGF localization was conducted on preparations of teased muscle fibers by transmission electron microscopy (TEM) and confocal microscopy (CM). Muscle interstitial fluid was sampled from microdialysis probes placed in the thigh muscle. TEM and CM analysis revealed two primary sites of localization of VEGF: in vesicles located in the subsarcolemmal regions and between the contractile elements within the muscle fibers; and in pericytes situated on the skeletal muscle capillaries. Quantitation of the subsarcolemmal density of VEGF vesicles, calculated on top of myonuclei, in the muscle fibers revealed a ∼50% increase (P
AB - The subcellular distribution and secretion of vascular endothelial growth factor (VEGF) was examined in skeletal muscle of healthy humans. Skeletal muscle biopsies were obtained from m.v. lateralis before and after a 2 h bout of cycling exercise. VEGF localization was conducted on preparations of teased muscle fibers by transmission electron microscopy (TEM) and confocal microscopy (CM). Muscle interstitial fluid was sampled from microdialysis probes placed in the thigh muscle. TEM and CM analysis revealed two primary sites of localization of VEGF: in vesicles located in the subsarcolemmal regions and between the contractile elements within the muscle fibers; and in pericytes situated on the skeletal muscle capillaries. Quantitation of the subsarcolemmal density of VEGF vesicles, calculated on top of myonuclei, in the muscle fibers revealed a ∼50% increase (P
U2 - 10.1096/fj.12-224618
DO - 10.1096/fj.12-224618
M3 - Journal article
C2 - 23709615
VL - 27
SP - 3496
EP - 3504
JO - F A S E B Journal
JF - F A S E B Journal
SN - 0892-6638
IS - 9
ER -
ID: 45895621