Sex hormone binding globulin phenotypes: their detection and distribution in healthy adults and in different clinical conditions

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Standard

Sex hormone binding globulin phenotypes : their detection and distribution in healthy adults and in different clinical conditions. / Cornelissea, Mette M; Bennetta, Paul E; Christiansen, Michael; Blaakaer, Jan; Gluud, Christian; Andersen, Jens Rikardt; Homann, Christian; Garred, Peter.

I: Clinica Chimica Acta, Bind 225, Nr. 2, 1994, s. 115-121.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Cornelissea, MM, Bennetta, PE, Christiansen, M, Blaakaer, J, Gluud, C, Andersen, JR, Homann, C & Garred, P 1994, 'Sex hormone binding globulin phenotypes: their detection and distribution in healthy adults and in different clinical conditions', Clinica Chimica Acta, bind 225, nr. 2, s. 115-121. https://doi.org/10.1016/0009-8981(94)90039-6

APA

Cornelissea, M. M., Bennetta, P. E., Christiansen, M., Blaakaer, J., Gluud, C., Andersen, J. R., Homann, C., & Garred, P. (1994). Sex hormone binding globulin phenotypes: their detection and distribution in healthy adults and in different clinical conditions. Clinica Chimica Acta, 225(2), 115-121. https://doi.org/10.1016/0009-8981(94)90039-6

Vancouver

Cornelissea MM, Bennetta PE, Christiansen M, Blaakaer J, Gluud C, Andersen JR o.a. Sex hormone binding globulin phenotypes: their detection and distribution in healthy adults and in different clinical conditions. Clinica Chimica Acta. 1994;225(2):115-121. https://doi.org/10.1016/0009-8981(94)90039-6

Author

Cornelissea, Mette M ; Bennetta, Paul E ; Christiansen, Michael ; Blaakaer, Jan ; Gluud, Christian ; Andersen, Jens Rikardt ; Homann, Christian ; Garred, Peter. / Sex hormone binding globulin phenotypes : their detection and distribution in healthy adults and in different clinical conditions. I: Clinica Chimica Acta. 1994 ; Bind 225, Nr. 2. s. 115-121.

Bibtex

@article{58775f1a2a544abaa81db2c1032d4bd5,
title = "Sex hormone binding globulin phenotypes: their detection and distribution in healthy adults and in different clinical conditions",
abstract = "Human sex hormone binding globulin (SHBG) is encoded by a normal and a variant allele. The resulting SHBG phenotypes (the homozygous normal SHBG, the heterozygous SHBG and the homozygous variant SHBG phenotype) can be distinguished by their electrophoretic patterns. We developed a novel detection system allowing us to distinguish between the different electrophoretic patterns in small amounts of plasma or serum (10 μ1). Small aliquots of Blue Sepharose were added to diluted sera or plasma samples for removal of albumin and the supernatants were subsequently applied to Western blotting. This method of detection was used to determine the distribution of SHBG phenotypes in healthy controls of both sexes and in five different pathological conditions characterized by changes in the SHBG level or endocrine disturbances (malignant and benign ovarian neoplasms, hirsutism, liver cirrhosis and alcoholism). The distribution of SHBG phenotypes was independent of such conditions and in agreement with the expected phenotype distribution of a bi-allelic gene in both healthy controls and patients (Hardy Weinberg law). An allele frequency of 0.13 was found for the SHBG variant allele based on the experimental values. Differences in SHBG phenotypes do not appear to have any clinical significance and no sex difference was found in the SHBG phenotype distribution.",
keywords = "Alcoholism, Carbohydrate heterogeneity, Electrophoresis, Hirsutism, Liver cirrhosis, Ovarian tumor, Sex hormone binding globulin phenotypes",
author = "Cornelissea, {Mette M} and Bennetta, {Paul E} and Michael Christiansen and Jan Blaakaer and Christian Gluud and Andersen, {Jens Rikardt} and Christian Homann and Peter Garred",
note = "(Ekstern)",
year = "1994",
doi = "10.1016/0009-8981(94)90039-6",
language = "English",
volume = "225",
pages = "115--121",
journal = "Clinica Chimica Acta",
issn = "0009-8981",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Sex hormone binding globulin phenotypes

T2 - their detection and distribution in healthy adults and in different clinical conditions

AU - Cornelissea, Mette M

AU - Bennetta, Paul E

AU - Christiansen, Michael

AU - Blaakaer, Jan

AU - Gluud, Christian

AU - Andersen, Jens Rikardt

AU - Homann, Christian

AU - Garred, Peter

N1 - (Ekstern)

PY - 1994

Y1 - 1994

N2 - Human sex hormone binding globulin (SHBG) is encoded by a normal and a variant allele. The resulting SHBG phenotypes (the homozygous normal SHBG, the heterozygous SHBG and the homozygous variant SHBG phenotype) can be distinguished by their electrophoretic patterns. We developed a novel detection system allowing us to distinguish between the different electrophoretic patterns in small amounts of plasma or serum (10 μ1). Small aliquots of Blue Sepharose were added to diluted sera or plasma samples for removal of albumin and the supernatants were subsequently applied to Western blotting. This method of detection was used to determine the distribution of SHBG phenotypes in healthy controls of both sexes and in five different pathological conditions characterized by changes in the SHBG level or endocrine disturbances (malignant and benign ovarian neoplasms, hirsutism, liver cirrhosis and alcoholism). The distribution of SHBG phenotypes was independent of such conditions and in agreement with the expected phenotype distribution of a bi-allelic gene in both healthy controls and patients (Hardy Weinberg law). An allele frequency of 0.13 was found for the SHBG variant allele based on the experimental values. Differences in SHBG phenotypes do not appear to have any clinical significance and no sex difference was found in the SHBG phenotype distribution.

AB - Human sex hormone binding globulin (SHBG) is encoded by a normal and a variant allele. The resulting SHBG phenotypes (the homozygous normal SHBG, the heterozygous SHBG and the homozygous variant SHBG phenotype) can be distinguished by their electrophoretic patterns. We developed a novel detection system allowing us to distinguish between the different electrophoretic patterns in small amounts of plasma or serum (10 μ1). Small aliquots of Blue Sepharose were added to diluted sera or plasma samples for removal of albumin and the supernatants were subsequently applied to Western blotting. This method of detection was used to determine the distribution of SHBG phenotypes in healthy controls of both sexes and in five different pathological conditions characterized by changes in the SHBG level or endocrine disturbances (malignant and benign ovarian neoplasms, hirsutism, liver cirrhosis and alcoholism). The distribution of SHBG phenotypes was independent of such conditions and in agreement with the expected phenotype distribution of a bi-allelic gene in both healthy controls and patients (Hardy Weinberg law). An allele frequency of 0.13 was found for the SHBG variant allele based on the experimental values. Differences in SHBG phenotypes do not appear to have any clinical significance and no sex difference was found in the SHBG phenotype distribution.

KW - Alcoholism

KW - Carbohydrate heterogeneity

KW - Electrophoresis

KW - Hirsutism

KW - Liver cirrhosis

KW - Ovarian tumor

KW - Sex hormone binding globulin phenotypes

UR - http://www.scopus.com/inward/record.url?scp=0028243833&partnerID=8YFLogxK

U2 - 10.1016/0009-8981(94)90039-6

DO - 10.1016/0009-8981(94)90039-6

M3 - Journal article

C2 - 8088001

AN - SCOPUS:0028243833

VL - 225

SP - 115

EP - 121

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

IS - 2

ER -

ID: 251989395