Role of cholesterol on the transfection barriers of cationic lipid/DNA complexes

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Role of cholesterol on the transfection barriers of cationic lipid/DNA complexes. / Pozzi, Daniela; Cardarelli, Francesco; Salomone, Fabrizio; Marchini, Cristina; Amenitsch, Heinz; La Barbera, Giorgia; Caracciolo, Giulio.

I: Applied Physics Letters, Bind 105, Nr. 7, 073701, 2014.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Pozzi, D, Cardarelli, F, Salomone, F, Marchini, C, Amenitsch, H, La Barbera, G & Caracciolo, G 2014, 'Role of cholesterol on the transfection barriers of cationic lipid/DNA complexes', Applied Physics Letters, bind 105, nr. 7, 073701. https://doi.org/10.1063/1.4892915

APA

Pozzi, D., Cardarelli, F., Salomone, F., Marchini, C., Amenitsch, H., La Barbera, G., & Caracciolo, G. (2014). Role of cholesterol on the transfection barriers of cationic lipid/DNA complexes. Applied Physics Letters, 105(7), [073701]. https://doi.org/10.1063/1.4892915

Vancouver

Pozzi D, Cardarelli F, Salomone F, Marchini C, Amenitsch H, La Barbera G o.a. Role of cholesterol on the transfection barriers of cationic lipid/DNA complexes. Applied Physics Letters. 2014;105(7). 073701. https://doi.org/10.1063/1.4892915

Author

Pozzi, Daniela ; Cardarelli, Francesco ; Salomone, Fabrizio ; Marchini, Cristina ; Amenitsch, Heinz ; La Barbera, Giorgia ; Caracciolo, Giulio. / Role of cholesterol on the transfection barriers of cationic lipid/DNA complexes. I: Applied Physics Letters. 2014 ; Bind 105, Nr. 7.

Bibtex

@article{e88f9ed1b5ba4478a2baa04c757bc089,
title = "Role of cholesterol on the transfection barriers of cationic lipid/DNA complexes",
abstract = "Most lipid formulations need cholesterol for efficient transfection, but the precise motivation remains unclear. Here, we have investigated the effect of cholesterol on the transfection efficiency (TE) of cationic liposomes made of 1,2-dioleoyl-3-trimethylammonium-propane and dioleoylphosphocholine in Chinese hamster ovary cells. The transfection mechanisms of cholesterol-containing lipoplexes have been investigated by TE, synchrotron small angle X-ray scattering, and laser scanning confocal microscopy experiments. We prove that cholesterol-containing lipoplexes enter the cells using different endocytosis pathways. Formulations with high cholesterol content efficiently escape from endosomes and exhibit a lamellar-nonlamellar phase transition in mixture with biomembrane mimicking lipid formulations. This might explain both the DNA release ability and the high transfection efficiency. These studies highlight the enrichment in cholesterol as a decisive factor for transfection and will contribute to the rational design of lipid nanocarriers with superior TE.",
author = "Daniela Pozzi and Francesco Cardarelli and Fabrizio Salomone and Cristina Marchini and Heinz Amenitsch and {La Barbera}, Giorgia and Giulio Caracciolo",
note = "(Ekstern)",
year = "2014",
doi = "10.1063/1.4892915",
language = "English",
volume = "105",
journal = "Applied Physics Letters",
issn = "0003-6951",
publisher = "American Institute of Physics",
number = "7",

}

RIS

TY - JOUR

T1 - Role of cholesterol on the transfection barriers of cationic lipid/DNA complexes

AU - Pozzi, Daniela

AU - Cardarelli, Francesco

AU - Salomone, Fabrizio

AU - Marchini, Cristina

AU - Amenitsch, Heinz

AU - La Barbera, Giorgia

AU - Caracciolo, Giulio

N1 - (Ekstern)

PY - 2014

Y1 - 2014

N2 - Most lipid formulations need cholesterol for efficient transfection, but the precise motivation remains unclear. Here, we have investigated the effect of cholesterol on the transfection efficiency (TE) of cationic liposomes made of 1,2-dioleoyl-3-trimethylammonium-propane and dioleoylphosphocholine in Chinese hamster ovary cells. The transfection mechanisms of cholesterol-containing lipoplexes have been investigated by TE, synchrotron small angle X-ray scattering, and laser scanning confocal microscopy experiments. We prove that cholesterol-containing lipoplexes enter the cells using different endocytosis pathways. Formulations with high cholesterol content efficiently escape from endosomes and exhibit a lamellar-nonlamellar phase transition in mixture with biomembrane mimicking lipid formulations. This might explain both the DNA release ability and the high transfection efficiency. These studies highlight the enrichment in cholesterol as a decisive factor for transfection and will contribute to the rational design of lipid nanocarriers with superior TE.

AB - Most lipid formulations need cholesterol for efficient transfection, but the precise motivation remains unclear. Here, we have investigated the effect of cholesterol on the transfection efficiency (TE) of cationic liposomes made of 1,2-dioleoyl-3-trimethylammonium-propane and dioleoylphosphocholine in Chinese hamster ovary cells. The transfection mechanisms of cholesterol-containing lipoplexes have been investigated by TE, synchrotron small angle X-ray scattering, and laser scanning confocal microscopy experiments. We prove that cholesterol-containing lipoplexes enter the cells using different endocytosis pathways. Formulations with high cholesterol content efficiently escape from endosomes and exhibit a lamellar-nonlamellar phase transition in mixture with biomembrane mimicking lipid formulations. This might explain both the DNA release ability and the high transfection efficiency. These studies highlight the enrichment in cholesterol as a decisive factor for transfection and will contribute to the rational design of lipid nanocarriers with superior TE.

UR - http://www.scopus.com/inward/record.url?scp=84929430382&partnerID=8YFLogxK

U2 - 10.1063/1.4892915

DO - 10.1063/1.4892915

M3 - Journal article

AN - SCOPUS:84929430382

VL - 105

JO - Applied Physics Letters

JF - Applied Physics Letters

SN - 0003-6951

IS - 7

M1 - 073701

ER -

ID: 231311193