Redox balance in human skeletal muscle-derived endothelial cells - Effect of exercise training

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Standard

Redox balance in human skeletal muscle-derived endothelial cells - Effect of exercise training. / Hansen, Camilla; Møller, Sophie; Ehlers, Thomas; Wickham, Kate Aiko; Bangsbo, Jens; Gliemann, Lasse; Hellsten, Ylva.

I: Free Radical Biology and Medicine, Bind 179, 2022, s. 144-155.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Hansen, C, Møller, S, Ehlers, T, Wickham, KA, Bangsbo, J, Gliemann, L & Hellsten, Y 2022, 'Redox balance in human skeletal muscle-derived endothelial cells - Effect of exercise training', Free Radical Biology and Medicine, bind 179, s. 144-155. https://doi.org/10.1016/j.freeradbiomed.2021.12.265

APA

Hansen, C., Møller, S., Ehlers, T., Wickham, K. A., Bangsbo, J., Gliemann, L., & Hellsten, Y. (2022). Redox balance in human skeletal muscle-derived endothelial cells - Effect of exercise training. Free Radical Biology and Medicine, 179, 144-155. https://doi.org/10.1016/j.freeradbiomed.2021.12.265

Vancouver

Hansen C, Møller S, Ehlers T, Wickham KA, Bangsbo J, Gliemann L o.a. Redox balance in human skeletal muscle-derived endothelial cells - Effect of exercise training. Free Radical Biology and Medicine. 2022;179:144-155. https://doi.org/10.1016/j.freeradbiomed.2021.12.265

Author

Hansen, Camilla ; Møller, Sophie ; Ehlers, Thomas ; Wickham, Kate Aiko ; Bangsbo, Jens ; Gliemann, Lasse ; Hellsten, Ylva. / Redox balance in human skeletal muscle-derived endothelial cells - Effect of exercise training. I: Free Radical Biology and Medicine. 2022 ; Bind 179. s. 144-155.

Bibtex

@article{8ff60b7ad7d043db83114c0ab5f66e28,
title = "Redox balance in human skeletal muscle-derived endothelial cells - Effect of exercise training",
abstract = "Aerobic training can improve vascular endothelial function in-vivo. The aim of this study was to elucidate the mechanisms underlying this improvement in isolated human microvascular endothelial cells. Sedentary males, aged 57 ± 6 years completed 8 weeks of intense aerobic training. Resting muscle biopsies were obtained from the thigh muscle and used for isolation of endothelial cells (pre n = 23, post n = 16). The cells were analyzed for mitochondrial respiration, H2O2 emission, glycolysis, protein levels of antioxidants, NADPH oxidase, endothelial nitric oxide (NO) synthase and prostacyclin synthase (PGI2S). In-vivo microvascular function, assessed by acetylcholine infusion and arterial blood pressure were also determined. Endothelial mitochondrial respiration and H2O2 formation were similar before and after training whereas the expression of superoxide dismutase and the expression of glutathione peroxidase were 2.4-fold (p = 0.012) and 2.3-fold (p = 0.006) higher, respectively, after training. In-vivo microvascular function was increased by 1.4-fold (p = 0.036) in parallel with a 2.1-fold increase in endothelial PGI2S expression (p = 0.041). Endothelial cell glycolysis was reduced after training, as indicated by a 65% lower basal production of lactate (p = 0.003) and a 30% lower expression of phosphofructokinase (p = 0.011). Subdivision of the participants according to blood pressure at base-line (n = 23), revealed a 2-fold higher (p = 0.049) rate of H2O2 production in endothelial cells from hypertensive participants. Our data show that exercise training increases skeletal muscle microvascular endothelial cell metabolism, antioxidant capacity and the capacity to form prostacyclin. Moreover, elevated blood pressure is associated with increased endothelial mitochondrial ROS formation.",
keywords = "Faculty of Science, Vascular function, Reactive oxygen species, Respirometry, Physical activity, Mitochondria",
author = "Camilla Hansen and Sophie M{\o}ller and Thomas Ehlers and Wickham, {Kate Aiko} and Jens Bangsbo and Lasse Gliemann and Ylva Hellsten",
note = "Copyright {\textcopyright} 2021. Published by Elsevier Inc.",
year = "2022",
doi = "10.1016/j.freeradbiomed.2021.12.265",
language = "English",
volume = "179",
pages = "144--155",
journal = "Free Radical Biology & Medicine",
issn = "0891-5849",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Redox balance in human skeletal muscle-derived endothelial cells - Effect of exercise training

AU - Hansen, Camilla

AU - Møller, Sophie

AU - Ehlers, Thomas

AU - Wickham, Kate Aiko

AU - Bangsbo, Jens

AU - Gliemann, Lasse

AU - Hellsten, Ylva

N1 - Copyright © 2021. Published by Elsevier Inc.

PY - 2022

Y1 - 2022

N2 - Aerobic training can improve vascular endothelial function in-vivo. The aim of this study was to elucidate the mechanisms underlying this improvement in isolated human microvascular endothelial cells. Sedentary males, aged 57 ± 6 years completed 8 weeks of intense aerobic training. Resting muscle biopsies were obtained from the thigh muscle and used for isolation of endothelial cells (pre n = 23, post n = 16). The cells were analyzed for mitochondrial respiration, H2O2 emission, glycolysis, protein levels of antioxidants, NADPH oxidase, endothelial nitric oxide (NO) synthase and prostacyclin synthase (PGI2S). In-vivo microvascular function, assessed by acetylcholine infusion and arterial blood pressure were also determined. Endothelial mitochondrial respiration and H2O2 formation were similar before and after training whereas the expression of superoxide dismutase and the expression of glutathione peroxidase were 2.4-fold (p = 0.012) and 2.3-fold (p = 0.006) higher, respectively, after training. In-vivo microvascular function was increased by 1.4-fold (p = 0.036) in parallel with a 2.1-fold increase in endothelial PGI2S expression (p = 0.041). Endothelial cell glycolysis was reduced after training, as indicated by a 65% lower basal production of lactate (p = 0.003) and a 30% lower expression of phosphofructokinase (p = 0.011). Subdivision of the participants according to blood pressure at base-line (n = 23), revealed a 2-fold higher (p = 0.049) rate of H2O2 production in endothelial cells from hypertensive participants. Our data show that exercise training increases skeletal muscle microvascular endothelial cell metabolism, antioxidant capacity and the capacity to form prostacyclin. Moreover, elevated blood pressure is associated with increased endothelial mitochondrial ROS formation.

AB - Aerobic training can improve vascular endothelial function in-vivo. The aim of this study was to elucidate the mechanisms underlying this improvement in isolated human microvascular endothelial cells. Sedentary males, aged 57 ± 6 years completed 8 weeks of intense aerobic training. Resting muscle biopsies were obtained from the thigh muscle and used for isolation of endothelial cells (pre n = 23, post n = 16). The cells were analyzed for mitochondrial respiration, H2O2 emission, glycolysis, protein levels of antioxidants, NADPH oxidase, endothelial nitric oxide (NO) synthase and prostacyclin synthase (PGI2S). In-vivo microvascular function, assessed by acetylcholine infusion and arterial blood pressure were also determined. Endothelial mitochondrial respiration and H2O2 formation were similar before and after training whereas the expression of superoxide dismutase and the expression of glutathione peroxidase were 2.4-fold (p = 0.012) and 2.3-fold (p = 0.006) higher, respectively, after training. In-vivo microvascular function was increased by 1.4-fold (p = 0.036) in parallel with a 2.1-fold increase in endothelial PGI2S expression (p = 0.041). Endothelial cell glycolysis was reduced after training, as indicated by a 65% lower basal production of lactate (p = 0.003) and a 30% lower expression of phosphofructokinase (p = 0.011). Subdivision of the participants according to blood pressure at base-line (n = 23), revealed a 2-fold higher (p = 0.049) rate of H2O2 production in endothelial cells from hypertensive participants. Our data show that exercise training increases skeletal muscle microvascular endothelial cell metabolism, antioxidant capacity and the capacity to form prostacyclin. Moreover, elevated blood pressure is associated with increased endothelial mitochondrial ROS formation.

KW - Faculty of Science

KW - Vascular function

KW - Reactive oxygen species

KW - Respirometry

KW - Physical activity

KW - Mitochondria

U2 - 10.1016/j.freeradbiomed.2021.12.265

DO - 10.1016/j.freeradbiomed.2021.12.265

M3 - Journal article

C2 - 34954023

VL - 179

SP - 144

EP - 155

JO - Free Radical Biology & Medicine

JF - Free Radical Biology & Medicine

SN - 0891-5849

ER -

ID: 288653072