Myosin heavy chain composition of single fibres from m. biceps brachii of male body builders
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Myosin heavy chain composition of single fibres from m. biceps brachii of male body builders. / Klitgaard, H; Zhou, M.-Y.; Richter, Erik A.
I: Acta Physiologica Scandinavica, Bind 140, Nr. 2, 1990, s. 175-180.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Myosin heavy chain composition of single fibres from m. biceps brachii of male body builders
AU - Klitgaard, H
AU - Zhou, M.-Y.
AU - Richter, Erik A.
PY - 1990
Y1 - 1990
N2 - The myosin heavy chain (MHC) composition of single fibres from m. biceps brachii of young sedentary men (28 +/- 0.4 years, mean +/- SE, n = 4) and male body builders (25 +/- 2.0 years, n = 4) was analysed with a sensitive one-dimensional electrophoretic technique. Compared with sedentary men, the body builders had a higher proportion of fibres containing only MHC type IIa (36 +/- 4 vs 12 +/- 2%; P less than 0.05), but a lower proportion of fibres with a coexistence of MHC types IIa and IIb (16 +/- 3 vs 34 +/- 2%; P less than 0.05) and nearly no fibres containing only MHC type IIb (1 +/- 1 vs 12 +/- 1%; P less than 0.05). Myofibrillar ATPase histochemistry only demonstrated a trend towards a higher proportion of type IIa fibres (31 +/- 6 vs 25 +/- 6%) and a lower proportion of type IIb fibres (18 +/- 5 vs 26 +/- 6%) within the body builders. These results, therefore, suggest an altered expression of MHC isoforms within histochemical type II fibres of human skeletal muscle with body building. Furthermore, in human skeletal muscle differences in expression of MHC isoforms may not always be reflected in the traditional histochemical classification of types I, IIa, IIb and IIc fibres.
AB - The myosin heavy chain (MHC) composition of single fibres from m. biceps brachii of young sedentary men (28 +/- 0.4 years, mean +/- SE, n = 4) and male body builders (25 +/- 2.0 years, n = 4) was analysed with a sensitive one-dimensional electrophoretic technique. Compared with sedentary men, the body builders had a higher proportion of fibres containing only MHC type IIa (36 +/- 4 vs 12 +/- 2%; P less than 0.05), but a lower proportion of fibres with a coexistence of MHC types IIa and IIb (16 +/- 3 vs 34 +/- 2%; P less than 0.05) and nearly no fibres containing only MHC type IIb (1 +/- 1 vs 12 +/- 1%; P less than 0.05). Myofibrillar ATPase histochemistry only demonstrated a trend towards a higher proportion of type IIa fibres (31 +/- 6 vs 25 +/- 6%) and a lower proportion of type IIb fibres (18 +/- 5 vs 26 +/- 6%) within the body builders. These results, therefore, suggest an altered expression of MHC isoforms within histochemical type II fibres of human skeletal muscle with body building. Furthermore, in human skeletal muscle differences in expression of MHC isoforms may not always be reflected in the traditional histochemical classification of types I, IIa, IIb and IIc fibres.
KW - Adenosine Triphosphatases
KW - Adult
KW - Electrophoresis
KW - Exercise
KW - Histocytochemistry
KW - Humans
KW - Male
KW - Muscles
KW - Myofibrils
KW - Myosins
U2 - 10.1111/j.1748-1716.1990.tb08989.x
DO - 10.1111/j.1748-1716.1990.tb08989.x
M3 - Journal article
C2 - 2148462
VL - 140
SP - 175
EP - 180
JO - Acta Physiologica Scandinavica
JF - Acta Physiologica Scandinavica
SN - 0001-6772
IS - 2
ER -
ID: 154755725