Membrane proteome functional characterization of breast cancer-initiating cells subjected to bone morphogenetic protein signaling inhibition by dorsomorphin

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Standard

Membrane proteome functional characterization of breast cancer-initiating cells subjected to bone morphogenetic protein signaling inhibition by dorsomorphin. / Piovesana, Susy; Capriotti, Anna Laura; Colapicchioni, Valentina; Ferraris, Francesca; La Barbera, Giorgia; Ventura, Salvatore.

I: Medicinal Chemistry Research, Bind 25, Nr. 9, 2016, s. 1971-1979.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Piovesana, S, Capriotti, AL, Colapicchioni, V, Ferraris, F, La Barbera, G & Ventura, S 2016, 'Membrane proteome functional characterization of breast cancer-initiating cells subjected to bone morphogenetic protein signaling inhibition by dorsomorphin', Medicinal Chemistry Research, bind 25, nr. 9, s. 1971-1979. https://doi.org/10.1007/s00044-016-1657-0

APA

Piovesana, S., Capriotti, A. L., Colapicchioni, V., Ferraris, F., La Barbera, G., & Ventura, S. (2016). Membrane proteome functional characterization of breast cancer-initiating cells subjected to bone morphogenetic protein signaling inhibition by dorsomorphin. Medicinal Chemistry Research, 25(9), 1971-1979. https://doi.org/10.1007/s00044-016-1657-0

Vancouver

Piovesana S, Capriotti AL, Colapicchioni V, Ferraris F, La Barbera G, Ventura S. Membrane proteome functional characterization of breast cancer-initiating cells subjected to bone morphogenetic protein signaling inhibition by dorsomorphin. Medicinal Chemistry Research. 2016;25(9):1971-1979. https://doi.org/10.1007/s00044-016-1657-0

Author

Piovesana, Susy ; Capriotti, Anna Laura ; Colapicchioni, Valentina ; Ferraris, Francesca ; La Barbera, Giorgia ; Ventura, Salvatore. / Membrane proteome functional characterization of breast cancer-initiating cells subjected to bone morphogenetic protein signaling inhibition by dorsomorphin. I: Medicinal Chemistry Research. 2016 ; Bind 25, Nr. 9. s. 1971-1979.

Bibtex

@article{15e77b57ea19446f8c84951bed1b47ad,
title = "Membrane proteome functional characterization of breast cancer-initiating cells subjected to bone morphogenetic protein signaling inhibition by dorsomorphin",
abstract = "In this study, A17 cells, which are an invasive mesenchymal cell line with cancer stem cell properties, were exploited for the study of the role of bone morphogenetic protein pathways in cancer-initiating cells employing a proteomics-based approach. A17 cells were treated with the bone morphogenetic protein signaling inhibitor dorsomorphin for 3 days. After that, subcellular fractionation of cell samples was performed and the membrane fraction analyzed by shotgun proteomics. The extracted membrane proteins were enzymatically digested and the resulting peptide mixture was analyzed by nano liquid chromatography coupled to tandem mass spectrometry and relative label-free quantitation. Protein profiles of A17 membrane fractions before and after dorsomorphin treatment were compared, and further mined by Gene Ontology search. The protein profile of untreated A17 samples correlated with the mesenchymal phenotype, whereas changes were observed in dorsomorphin-treated samples, further supporting a mesenchymal to epithelial transition upon bone morphogenetic protein signaling pathway inhibition and the importance of this pathway in breast cancer cell malignancy.",
keywords = "Bone morphogenetic protein signaling inhibition, Breast cancer, Dorsomorphin, Mass spectrometry, Mesenchymal-epithelial transition, Proteomics, Spectral counting",
author = "Susy Piovesana and Capriotti, {Anna Laura} and Valentina Colapicchioni and Francesca Ferraris and {La Barbera}, Giorgia and Salvatore Ventura",
note = "(Ekstern)",
year = "2016",
doi = "10.1007/s00044-016-1657-0",
language = "English",
volume = "25",
pages = "1971--1979",
journal = "Medicinal Chemistry Research",
issn = "1054-2523",
publisher = "Springer Basel AG",
number = "9",

}

RIS

TY - JOUR

T1 - Membrane proteome functional characterization of breast cancer-initiating cells subjected to bone morphogenetic protein signaling inhibition by dorsomorphin

AU - Piovesana, Susy

AU - Capriotti, Anna Laura

AU - Colapicchioni, Valentina

AU - Ferraris, Francesca

AU - La Barbera, Giorgia

AU - Ventura, Salvatore

N1 - (Ekstern)

PY - 2016

Y1 - 2016

N2 - In this study, A17 cells, which are an invasive mesenchymal cell line with cancer stem cell properties, were exploited for the study of the role of bone morphogenetic protein pathways in cancer-initiating cells employing a proteomics-based approach. A17 cells were treated with the bone morphogenetic protein signaling inhibitor dorsomorphin for 3 days. After that, subcellular fractionation of cell samples was performed and the membrane fraction analyzed by shotgun proteomics. The extracted membrane proteins were enzymatically digested and the resulting peptide mixture was analyzed by nano liquid chromatography coupled to tandem mass spectrometry and relative label-free quantitation. Protein profiles of A17 membrane fractions before and after dorsomorphin treatment were compared, and further mined by Gene Ontology search. The protein profile of untreated A17 samples correlated with the mesenchymal phenotype, whereas changes were observed in dorsomorphin-treated samples, further supporting a mesenchymal to epithelial transition upon bone morphogenetic protein signaling pathway inhibition and the importance of this pathway in breast cancer cell malignancy.

AB - In this study, A17 cells, which are an invasive mesenchymal cell line with cancer stem cell properties, were exploited for the study of the role of bone morphogenetic protein pathways in cancer-initiating cells employing a proteomics-based approach. A17 cells were treated with the bone morphogenetic protein signaling inhibitor dorsomorphin for 3 days. After that, subcellular fractionation of cell samples was performed and the membrane fraction analyzed by shotgun proteomics. The extracted membrane proteins were enzymatically digested and the resulting peptide mixture was analyzed by nano liquid chromatography coupled to tandem mass spectrometry and relative label-free quantitation. Protein profiles of A17 membrane fractions before and after dorsomorphin treatment were compared, and further mined by Gene Ontology search. The protein profile of untreated A17 samples correlated with the mesenchymal phenotype, whereas changes were observed in dorsomorphin-treated samples, further supporting a mesenchymal to epithelial transition upon bone morphogenetic protein signaling pathway inhibition and the importance of this pathway in breast cancer cell malignancy.

KW - Bone morphogenetic protein signaling inhibition

KW - Breast cancer

KW - Dorsomorphin

KW - Mass spectrometry

KW - Mesenchymal-epithelial transition

KW - Proteomics

KW - Spectral counting

UR - http://www.scopus.com/inward/record.url?scp=84978745441&partnerID=8YFLogxK

U2 - 10.1007/s00044-016-1657-0

DO - 10.1007/s00044-016-1657-0

M3 - Journal article

AN - SCOPUS:84978745441

VL - 25

SP - 1971

EP - 1979

JO - Medicinal Chemistry Research

JF - Medicinal Chemistry Research

SN - 1054-2523

IS - 9

ER -

ID: 231312018