Investigation of free and conjugated seleno-amino acids in wheat bran by hydrophilic interaction liquid chromatography with tandem mass spectrometry

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Standard

Investigation of free and conjugated seleno-amino acids in wheat bran by hydrophilic interaction liquid chromatography with tandem mass spectrometry. / Montone, Carmela Maria; Antonelli, Michela; Capriotti, Anna Laura; Cavaliere, Chiara; La Barbera, Giorgia; Piovesana, Susy; Laganà, Aldo.

I: Journal of Separation Science, Bind 42, Nr. 10, 2019, s. 1938-1947.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Montone, CM, Antonelli, M, Capriotti, AL, Cavaliere, C, La Barbera, G, Piovesana, S & Laganà, A 2019, 'Investigation of free and conjugated seleno-amino acids in wheat bran by hydrophilic interaction liquid chromatography with tandem mass spectrometry', Journal of Separation Science, bind 42, nr. 10, s. 1938-1947. https://doi.org/10.1002/jssc.201900047

APA

Montone, C. M., Antonelli, M., Capriotti, A. L., Cavaliere, C., La Barbera, G., Piovesana, S., & Laganà, A. (2019). Investigation of free and conjugated seleno-amino acids in wheat bran by hydrophilic interaction liquid chromatography with tandem mass spectrometry. Journal of Separation Science, 42(10), 1938-1947. https://doi.org/10.1002/jssc.201900047

Vancouver

Montone CM, Antonelli M, Capriotti AL, Cavaliere C, La Barbera G, Piovesana S o.a. Investigation of free and conjugated seleno-amino acids in wheat bran by hydrophilic interaction liquid chromatography with tandem mass spectrometry. Journal of Separation Science. 2019;42(10):1938-1947. https://doi.org/10.1002/jssc.201900047

Author

Montone, Carmela Maria ; Antonelli, Michela ; Capriotti, Anna Laura ; Cavaliere, Chiara ; La Barbera, Giorgia ; Piovesana, Susy ; Laganà, Aldo. / Investigation of free and conjugated seleno-amino acids in wheat bran by hydrophilic interaction liquid chromatography with tandem mass spectrometry. I: Journal of Separation Science. 2019 ; Bind 42, Nr. 10. s. 1938-1947.

Bibtex

@article{11ca4d43b6014be5a1f6444baf819420,
title = "Investigation of free and conjugated seleno-amino acids in wheat bran by hydrophilic interaction liquid chromatography with tandem mass spectrometry",
abstract = "An analytical method for determining seleno-methionine, methyl-seleno-cysteine, and seleno-cystine in wheat bran was developed and validated. Four different extraction procedures were evaluated to simultaneously extract endogenous free and conjugated seleno-amino acids in wheat bran in order to select the best extraction protocol in terms of seleno amino acid quantitation. The extracted samples were subjected to a clean-up by a reversed phase/strong cation exchange solid-phase extraction and analyzed by chiral hydrophilic interaction liquid chromatography-tandem mass spectrometry. The optimized extraction protocol was employed to validate the methodology. Process efficiency ranged from 58 to 112% and trueness from 73 to 98%. Limit of detection and limit of quantification were lower than 1 ng/g. Four wheat bran samples were analyzed for both total Se and single seleno-amino acids determination. The results showed that Se- seleno-methyl-lselenocysteine was the major seleno-amino acid in wheat bran while seleno-methionine and seleno-cysteine were both minor species.",
keywords = "Enantioselective high-performance liquid chromatography, Mass spectrometry, Seleno-amino acids, Total selenium, Wheat bran",
author = "Montone, {Carmela Maria} and Michela Antonelli and Capriotti, {Anna Laura} and Chiara Cavaliere and {La Barbera}, Giorgia and Susy Piovesana and Aldo Lagan{\`a}",
note = "(Ekstern)",
year = "2019",
doi = "10.1002/jssc.201900047",
language = "English",
volume = "42",
pages = "1938--1947",
journal = "HRC & CC, Journal of High Resolution Chromatography and Chromatography Communications",
issn = "1615-9306",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "10",

}

RIS

TY - JOUR

T1 - Investigation of free and conjugated seleno-amino acids in wheat bran by hydrophilic interaction liquid chromatography with tandem mass spectrometry

AU - Montone, Carmela Maria

AU - Antonelli, Michela

AU - Capriotti, Anna Laura

AU - Cavaliere, Chiara

AU - La Barbera, Giorgia

AU - Piovesana, Susy

AU - Laganà, Aldo

N1 - (Ekstern)

PY - 2019

Y1 - 2019

N2 - An analytical method for determining seleno-methionine, methyl-seleno-cysteine, and seleno-cystine in wheat bran was developed and validated. Four different extraction procedures were evaluated to simultaneously extract endogenous free and conjugated seleno-amino acids in wheat bran in order to select the best extraction protocol in terms of seleno amino acid quantitation. The extracted samples were subjected to a clean-up by a reversed phase/strong cation exchange solid-phase extraction and analyzed by chiral hydrophilic interaction liquid chromatography-tandem mass spectrometry. The optimized extraction protocol was employed to validate the methodology. Process efficiency ranged from 58 to 112% and trueness from 73 to 98%. Limit of detection and limit of quantification were lower than 1 ng/g. Four wheat bran samples were analyzed for both total Se and single seleno-amino acids determination. The results showed that Se- seleno-methyl-lselenocysteine was the major seleno-amino acid in wheat bran while seleno-methionine and seleno-cysteine were both minor species.

AB - An analytical method for determining seleno-methionine, methyl-seleno-cysteine, and seleno-cystine in wheat bran was developed and validated. Four different extraction procedures were evaluated to simultaneously extract endogenous free and conjugated seleno-amino acids in wheat bran in order to select the best extraction protocol in terms of seleno amino acid quantitation. The extracted samples were subjected to a clean-up by a reversed phase/strong cation exchange solid-phase extraction and analyzed by chiral hydrophilic interaction liquid chromatography-tandem mass spectrometry. The optimized extraction protocol was employed to validate the methodology. Process efficiency ranged from 58 to 112% and trueness from 73 to 98%. Limit of detection and limit of quantification were lower than 1 ng/g. Four wheat bran samples were analyzed for both total Se and single seleno-amino acids determination. The results showed that Se- seleno-methyl-lselenocysteine was the major seleno-amino acid in wheat bran while seleno-methionine and seleno-cysteine were both minor species.

KW - Enantioselective high-performance liquid chromatography

KW - Mass spectrometry

KW - Seleno-amino acids

KW - Total selenium

KW - Wheat bran

UR - http://www.scopus.com/inward/record.url?scp=85064495322&partnerID=8YFLogxK

U2 - 10.1002/jssc.201900047

DO - 10.1002/jssc.201900047

M3 - Journal article

C2 - 30920149

AN - SCOPUS:85064495322

VL - 42

SP - 1938

EP - 1947

JO - HRC & CC, Journal of High Resolution Chromatography and Chromatography Communications

JF - HRC & CC, Journal of High Resolution Chromatography and Chromatography Communications

SN - 1615-9306

IS - 10

ER -

ID: 231310760