Investigation of free and conjugated seleno-amino acids in wheat bran by hydrophilic interaction liquid chromatography with tandem mass spectrometry
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Investigation of free and conjugated seleno-amino acids in wheat bran by hydrophilic interaction liquid chromatography with tandem mass spectrometry. / Montone, Carmela Maria; Antonelli, Michela; Capriotti, Anna Laura; Cavaliere, Chiara; La Barbera, Giorgia; Piovesana, Susy; Laganà, Aldo.
I: Journal of Separation Science, Bind 42, Nr. 10, 2019, s. 1938-1947.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
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TY - JOUR
T1 - Investigation of free and conjugated seleno-amino acids in wheat bran by hydrophilic interaction liquid chromatography with tandem mass spectrometry
AU - Montone, Carmela Maria
AU - Antonelli, Michela
AU - Capriotti, Anna Laura
AU - Cavaliere, Chiara
AU - La Barbera, Giorgia
AU - Piovesana, Susy
AU - Laganà, Aldo
N1 - (Ekstern)
PY - 2019
Y1 - 2019
N2 - An analytical method for determining seleno-methionine, methyl-seleno-cysteine, and seleno-cystine in wheat bran was developed and validated. Four different extraction procedures were evaluated to simultaneously extract endogenous free and conjugated seleno-amino acids in wheat bran in order to select the best extraction protocol in terms of seleno amino acid quantitation. The extracted samples were subjected to a clean-up by a reversed phase/strong cation exchange solid-phase extraction and analyzed by chiral hydrophilic interaction liquid chromatography-tandem mass spectrometry. The optimized extraction protocol was employed to validate the methodology. Process efficiency ranged from 58 to 112% and trueness from 73 to 98%. Limit of detection and limit of quantification were lower than 1 ng/g. Four wheat bran samples were analyzed for both total Se and single seleno-amino acids determination. The results showed that Se- seleno-methyl-lselenocysteine was the major seleno-amino acid in wheat bran while seleno-methionine and seleno-cysteine were both minor species.
AB - An analytical method for determining seleno-methionine, methyl-seleno-cysteine, and seleno-cystine in wheat bran was developed and validated. Four different extraction procedures were evaluated to simultaneously extract endogenous free and conjugated seleno-amino acids in wheat bran in order to select the best extraction protocol in terms of seleno amino acid quantitation. The extracted samples were subjected to a clean-up by a reversed phase/strong cation exchange solid-phase extraction and analyzed by chiral hydrophilic interaction liquid chromatography-tandem mass spectrometry. The optimized extraction protocol was employed to validate the methodology. Process efficiency ranged from 58 to 112% and trueness from 73 to 98%. Limit of detection and limit of quantification were lower than 1 ng/g. Four wheat bran samples were analyzed for both total Se and single seleno-amino acids determination. The results showed that Se- seleno-methyl-lselenocysteine was the major seleno-amino acid in wheat bran while seleno-methionine and seleno-cysteine were both minor species.
KW - Enantioselective high-performance liquid chromatography
KW - Mass spectrometry
KW - Seleno-amino acids
KW - Total selenium
KW - Wheat bran
UR - http://www.scopus.com/inward/record.url?scp=85064495322&partnerID=8YFLogxK
U2 - 10.1002/jssc.201900047
DO - 10.1002/jssc.201900047
M3 - Journal article
C2 - 30920149
AN - SCOPUS:85064495322
VL - 42
SP - 1938
EP - 1947
JO - HRC & CC, Journal of High Resolution Chromatography and Chromatography Communications
JF - HRC & CC, Journal of High Resolution Chromatography and Chromatography Communications
SN - 1615-9306
IS - 10
ER -
ID: 231310760