TY - JOUR

T1 - Influence of dynamic flow environment on nanoparticle-protein corona

T2 - From protein patterns to uptake in cancer cells

AU - Palchetti, Sara

AU - Pozzi, Daniela

AU - Capriotti, Anna Laura

AU - La Barbera, Giorgia

AU - Zenezini Chiozzi, Riccardo

AU - Digiacomo, Luca

AU - Peruzzi, Giovanna

AU - Caracciolo, Giulio

AU - Laganà, Aldo

N1 - (Ekstern)

PY - 2017

Y1 - 2017

N2 - The fast growing use of nanoparticles (NPs) in biotechnology and biomedicine raises concerns about human health and the environment. When introduced in physiological milieus, NPs adsorb biomolecules (especially proteins) forming the so-called protein corona (PC). As it is the PC that mostly interacts with biological systems, it represents a major element of the NPs’ biological identity with impact on nanotoxicology, nanosafety and targeted delivery of nanomedicines. To date, NP-protein interactions have been largely investigated in vitro, but this condition is far from mimicking the dynamic nature of physiological environments. Here we investigate the effect of shear stress on PC by exposing lipid NPs with different surface chemistry (either unmodified and PEGylated) to circulating fetal bovine serum (FBS). PC formed upon in vitro incubation was used as a reference. We demonstrate that PC is significantly influenced by exposure to dynamic flow and that changes in PC composition are dependent on both exposure time and NP's surface chemistry. Notably, alterations induced by dynamic flow affected cellular uptake of lipid NPs in both human cervical cancer (HeLa) and human breast adenocarcinoma (MCF7) cell lines.

AB - The fast growing use of nanoparticles (NPs) in biotechnology and biomedicine raises concerns about human health and the environment. When introduced in physiological milieus, NPs adsorb biomolecules (especially proteins) forming the so-called protein corona (PC). As it is the PC that mostly interacts with biological systems, it represents a major element of the NPs’ biological identity with impact on nanotoxicology, nanosafety and targeted delivery of nanomedicines. To date, NP-protein interactions have been largely investigated in vitro, but this condition is far from mimicking the dynamic nature of physiological environments. Here we investigate the effect of shear stress on PC by exposing lipid NPs with different surface chemistry (either unmodified and PEGylated) to circulating fetal bovine serum (FBS). PC formed upon in vitro incubation was used as a reference. We demonstrate that PC is significantly influenced by exposure to dynamic flow and that changes in PC composition are dependent on both exposure time and NP's surface chemistry. Notably, alterations induced by dynamic flow affected cellular uptake of lipid NPs in both human cervical cancer (HeLa) and human breast adenocarcinoma (MCF7) cell lines.

KW - Cancer cells

KW - Dynamic flow environment

KW - Nanoparticles

KW - Physiological environments

KW - Protein corona

UR - http://www.scopus.com/inward/record.url?scp=85014354605&partnerID=8YFLogxK

U2 - 10.1016/j.colsurfb.2017.02.037

DO - 10.1016/j.colsurfb.2017.02.037

M3 - Journal article

C2 - 28273493

AN - SCOPUS:85014354605

VL - 153

SP - 263

EP - 271

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

ER -