Impact of training state on fasting-induced regulation of adipose tissue metabolism in humans
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Impact of training state on fasting-induced regulation of adipose tissue metabolism in humans. / Bertholdt, Lærke; Gudiksen, Anders; Stankiewicz, Tomasz; Villesen, Ida; Tybirk, Jonas; van Hall, Gerrit; Bangsbo, Jens; Plomgaard, Peter; Pilegaard, Henriette.
I: Journal of Applied Physiology, Bind 124, Nr. 3, 2018, s. 729-740.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Impact of training state on fasting-induced regulation of adipose tissue metabolism in humans
AU - Bertholdt, Lærke
AU - Gudiksen, Anders
AU - Stankiewicz, Tomasz
AU - Villesen, Ida
AU - Tybirk, Jonas
AU - van Hall, Gerrit
AU - Bangsbo, Jens
AU - Plomgaard, Peter
AU - Pilegaard, Henriette
N1 - CURIS 2018 NEXS 145
PY - 2018
Y1 - 2018
N2 - Recruitment of fatty acids from adipose tissue is essential during fasting. However, the molecular mechanisms behind fasting-induced metabolic regulation in human adipose tissue and the potential impact of training state in this are unknown. Therefore, the aim of the present study was to investigate 1) fasting-induced regulation of lipolysis and glyceroneogenesis in human adipose tissue as well as 2) the impact of training state on basal oxidative capacity and fasting-induced metabolic regulation in human adipose tissue. Untrained (VO2max < 45ml·min-1·kg-1) and trained subjects (VO2max > 55ml·min-1·kg-1) fasted for 36h and abdominal subcutaneous adipose tissue biopsies were obtained 2, 12, 24 and 36h after a standardized meal. Adipose tissue OXPHOS, phosphoenolpyruvate carboxykinase and pyruvate dehydrogenase (PDH) E1α protein as well as PDH kinase (PDK) 2, PDK4 and PDH phosphatase 2 mRNA content were higher in trained subjects than untrained subjects. In addition, trained subjects had higher adipose tissue hormone sensitive lipase Ser660 phosphorylation and adipose triglyceride lipase protein content as well as higher plasma free fatty acids concentration than untrained subjects during fasting. Moreover, adipose tissue PDH phosphorylation increased with fasting only in trained subjects. Taken together, trained subjects seem to possess higher basal adipose tissue oxidative capacity as well as higher capacity for regulation of lipolysis, glyceroneogenesis and substrate availability in adipose tissue than untrained subjects.
AB - Recruitment of fatty acids from adipose tissue is essential during fasting. However, the molecular mechanisms behind fasting-induced metabolic regulation in human adipose tissue and the potential impact of training state in this are unknown. Therefore, the aim of the present study was to investigate 1) fasting-induced regulation of lipolysis and glyceroneogenesis in human adipose tissue as well as 2) the impact of training state on basal oxidative capacity and fasting-induced metabolic regulation in human adipose tissue. Untrained (VO2max < 45ml·min-1·kg-1) and trained subjects (VO2max > 55ml·min-1·kg-1) fasted for 36h and abdominal subcutaneous adipose tissue biopsies were obtained 2, 12, 24 and 36h after a standardized meal. Adipose tissue OXPHOS, phosphoenolpyruvate carboxykinase and pyruvate dehydrogenase (PDH) E1α protein as well as PDH kinase (PDK) 2, PDK4 and PDH phosphatase 2 mRNA content were higher in trained subjects than untrained subjects. In addition, trained subjects had higher adipose tissue hormone sensitive lipase Ser660 phosphorylation and adipose triglyceride lipase protein content as well as higher plasma free fatty acids concentration than untrained subjects during fasting. Moreover, adipose tissue PDH phosphorylation increased with fasting only in trained subjects. Taken together, trained subjects seem to possess higher basal adipose tissue oxidative capacity as well as higher capacity for regulation of lipolysis, glyceroneogenesis and substrate availability in adipose tissue than untrained subjects.
KW - Fasting
KW - Exercise training
KW - Adipose tissue
KW - Lipolysis
KW - Glyceroneogenesis
U2 - 10.1152/japplphysiol.00664.2017
DO - 10.1152/japplphysiol.00664.2017
M3 - Journal article
C2 - 29191981
VL - 124
SP - 729
EP - 740
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 3
ER -
ID: 186525727