Exercise-induced increase in glucose transport, GLUT-4, and VAMP-2 in plasma membrane from human muscle

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Standard

Exercise-induced increase in glucose transport, GLUT-4, and VAMP-2 in plasma membrane from human muscle. / Kristiansen, S; Hargreaves, Mark; Richter, Erik A.

I: American Journal of Physiology: Endocrinology and Metabolism, Bind 270, Nr. 1, 1996, s. E197-E201.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Kristiansen, S, Hargreaves, M & Richter, EA 1996, 'Exercise-induced increase in glucose transport, GLUT-4, and VAMP-2 in plasma membrane from human muscle', American Journal of Physiology: Endocrinology and Metabolism, bind 270, nr. 1, s. E197-E201.

APA

Kristiansen, S., Hargreaves, M., & Richter, E. A. (1996). Exercise-induced increase in glucose transport, GLUT-4, and VAMP-2 in plasma membrane from human muscle. American Journal of Physiology: Endocrinology and Metabolism, 270(1), E197-E201.

Vancouver

Kristiansen S, Hargreaves M, Richter EA. Exercise-induced increase in glucose transport, GLUT-4, and VAMP-2 in plasma membrane from human muscle. American Journal of Physiology: Endocrinology and Metabolism. 1996;270(1):E197-E201.

Author

Kristiansen, S ; Hargreaves, Mark ; Richter, Erik A. / Exercise-induced increase in glucose transport, GLUT-4, and VAMP-2 in plasma membrane from human muscle. I: American Journal of Physiology: Endocrinology and Metabolism. 1996 ; Bind 270, Nr. 1. s. E197-E201.

Bibtex

@article{a6bfa87ab0a34a47987aa1432a579d1f,
title = "Exercise-induced increase in glucose transport, GLUT-4, and VAMP-2 in plasma membrane from human muscle",
abstract = "A major effect of muscle contractions is an increase in sarcolemmal glucose transport. We have used a recently developed technique to produce sarcolemmal giant vesicles from human muscle biopsy samples obtained before and after exercise. Six men exercised for 10 min at 50% maximal O2 uptake (Vo2max) and then to fatigue at 100% Vo2max (5.7 +/- 0.2 min). Vesicle glucose transport at 5 mM increased from 3.3 +/- 0.6 pmol.microgram-1.min-1 at rest to 6.6 +/- 1.0 pmol.microgram-1.min-1 at fatigue (mean +/- SE, n = 6, P < 0.05). This increase in glucose transport was associated with a 1.6-fold increase in vesicle GLUT-4 protein content. Glucose transport normalized to GLUT-4 protein content also increased with exercise, suggesting increased intrinsic activity of GLUT-4. Furthermore, exercise resulted in a 1.4-fold increase in sarcolemmal vesicle-associated membrane protein (VAMP-2) content, suggesting that muscle contractions may induce trafficking of GLUT-4-containing vesicles via a mechanism similar to neurotransmitter release. Our results demonstrate for the first time exercise-induced translocation of GLUT-4 and VAMP-2 to the plasma membrane of human muscle and increased sarcolemmal glucose transport.",
keywords = "Adult, Biological Transport, Blotting, Western, Cell Membrane, Glucose, Glucose Transporter Type 4, Humans, Male, Membrane Proteins, Monosaccharide Transport Proteins, Muscle Proteins, Muscles, Physical Exertion, R-SNARE Proteins, Sarcolemma",
author = "S Kristiansen and Mark Hargreaves and Richter, {Erik A.}",
year = "1996",
language = "English",
volume = "270",
pages = "E197--E201",
journal = "American Journal of Physiology: Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "1",

}

RIS

TY - JOUR

T1 - Exercise-induced increase in glucose transport, GLUT-4, and VAMP-2 in plasma membrane from human muscle

AU - Kristiansen, S

AU - Hargreaves, Mark

AU - Richter, Erik A.

PY - 1996

Y1 - 1996

N2 - A major effect of muscle contractions is an increase in sarcolemmal glucose transport. We have used a recently developed technique to produce sarcolemmal giant vesicles from human muscle biopsy samples obtained before and after exercise. Six men exercised for 10 min at 50% maximal O2 uptake (Vo2max) and then to fatigue at 100% Vo2max (5.7 +/- 0.2 min). Vesicle glucose transport at 5 mM increased from 3.3 +/- 0.6 pmol.microgram-1.min-1 at rest to 6.6 +/- 1.0 pmol.microgram-1.min-1 at fatigue (mean +/- SE, n = 6, P < 0.05). This increase in glucose transport was associated with a 1.6-fold increase in vesicle GLUT-4 protein content. Glucose transport normalized to GLUT-4 protein content also increased with exercise, suggesting increased intrinsic activity of GLUT-4. Furthermore, exercise resulted in a 1.4-fold increase in sarcolemmal vesicle-associated membrane protein (VAMP-2) content, suggesting that muscle contractions may induce trafficking of GLUT-4-containing vesicles via a mechanism similar to neurotransmitter release. Our results demonstrate for the first time exercise-induced translocation of GLUT-4 and VAMP-2 to the plasma membrane of human muscle and increased sarcolemmal glucose transport.

AB - A major effect of muscle contractions is an increase in sarcolemmal glucose transport. We have used a recently developed technique to produce sarcolemmal giant vesicles from human muscle biopsy samples obtained before and after exercise. Six men exercised for 10 min at 50% maximal O2 uptake (Vo2max) and then to fatigue at 100% Vo2max (5.7 +/- 0.2 min). Vesicle glucose transport at 5 mM increased from 3.3 +/- 0.6 pmol.microgram-1.min-1 at rest to 6.6 +/- 1.0 pmol.microgram-1.min-1 at fatigue (mean +/- SE, n = 6, P < 0.05). This increase in glucose transport was associated with a 1.6-fold increase in vesicle GLUT-4 protein content. Glucose transport normalized to GLUT-4 protein content also increased with exercise, suggesting increased intrinsic activity of GLUT-4. Furthermore, exercise resulted in a 1.4-fold increase in sarcolemmal vesicle-associated membrane protein (VAMP-2) content, suggesting that muscle contractions may induce trafficking of GLUT-4-containing vesicles via a mechanism similar to neurotransmitter release. Our results demonstrate for the first time exercise-induced translocation of GLUT-4 and VAMP-2 to the plasma membrane of human muscle and increased sarcolemmal glucose transport.

KW - Adult

KW - Biological Transport

KW - Blotting, Western

KW - Cell Membrane

KW - Glucose

KW - Glucose Transporter Type 4

KW - Humans

KW - Male

KW - Membrane Proteins

KW - Monosaccharide Transport Proteins

KW - Muscle Proteins

KW - Muscles

KW - Physical Exertion

KW - R-SNARE Proteins

KW - Sarcolemma

M3 - Journal article

C2 - 8772493

VL - 270

SP - E197-E201

JO - American Journal of Physiology: Endocrinology and Metabolism

JF - American Journal of Physiology: Endocrinology and Metabolism

SN - 0193-1849

IS - 1

ER -

ID: 154750965